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期 刊 ：植物保护学报 2008年

关键词：内生真菌；紫杉木霉；代谢产物；理化性质；

Keywords:endophytic fungi, Trichoderma taxi, metabolite, physical and chemical property,

摘 要 ：内生真菌紫杉木霉ZJUF0986菌株产生的活性代谢产物Ⅰ对植物病原真菌具有广谱抑菌活性。通过紫外吸收、薄层色谱、捷克八溶剂系统纸色谱、pH纸色谱及不同pH和温度处理手段,研究了该活性代谢产物Ⅰ的部分理化性质。结果表明,活性代谢产物Ⅰ的最大紫外吸收峰为199nm;以石油醚∶ 乙酸乙酯=95∶ 5为展开剂薄层层析时,样品斑点的Rf值为0.58;捷克八溶剂系统纸色谱和pH纸色谱图谱与已知各类抗生素纸图谱相比较,活性代谢产物Ⅰ是一类低极性、弱酸性、非水溶性的抗生素;活性代谢产物Ⅰ在酸性至弱碱性和低于100℃的条件下相当稳定,在pH＞10和121℃下活性物质不稳定。

Abstract:Bioactive metabolite I produced by endophytic fungus Trichoderma taxi ZJUF0986 displays broad antifungal activity to many phytopathogenic fungi. The partial physical and chemical properties of the bioactive metabolite I was analyzed by ultraviolet absorbability, thin-layer chromatography, paper chromatography pH and Doskochilova system, and treatment with different pH and temperature levels. Results showed that the maximum UV absorb wavelength was 199nm. The Rf value of the metabolite I was 0.58 when thin-layer chromatography in petroleum ether∶ ethyl acetate (95∶ 5) as mobile phase. The comparison of paper chromatography pH and Doskochilova system between the metabolite I and various known antibiotics indicated that the metabolite I was a low polar, weakly acerbic and hydrophobic antibiotic. And the metabolite I maintained stability at acidic to alkalescent and low than 100℃ conditions, it becomes unstable at pH>10 or 121℃.

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