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期 刊 ：植物保护学报 2010年

关键词：小麦-柔软滨麦草易位系；抗条锈性；遗传分析；SSR标记；

Keywords:wheat-translocation line (Leymus mollis), stripe rust resistance, genetic analysis, SSR marker,

摘 要 ：为揭示小麦-柔软滨麦草易位系M853-4的抗条锈性遗传机制,以易位系M853-4和感病品种铭贤169为亲本制备F₂、F₃代种子,采用人工接种的方法于温室中接种小麦条锈菌生理小种Su-11,用于测定M853-4及其杂交后代的苗期抗条锈性。结果表明,M853-4对Su-11的抗病性由1对显性和1对隐性基因控制。筛选由1对显性基因控制的F₃代分离家系作为SSR标记群体,从320对引物中共找到了4个位于4A染色体上的与该显性基因(暂命名为YrLm2)紧密连锁的微卫星标记Xgwm44、Xwmc650、Barc170和Xwmc718,标记到YrLm2的遗传距离分别为15.0、5.0、3.9和3.1cm,并将YrLm2定位于4A染色体的长臂上,标记结果可用于小麦分子辅助育种。

Abstract:To reveal mechanism of seedling resistance to stripe rust in wheat translocation line M853-4 derived from Leymus mollis (Trin.) Hara, F₂, F₃ seeds derived from the cross of the wheat translocation line M853-4 and a susceptible variety Mingxian 169, and seedling resistance of M853-4 and it’s progenies to stripe rust was analyzed by artificial inoculation with races of the pathogen Su-11 in the greenhouse. The results suggested that M853-4 had one dominant gene and one recessive gene conferred resistance to Su-11. Using one segregation line from F₃ family which had one dominant gene to Su-11 as SSR mapping population, four SSR markers, Xgwm44, Xwmc650, Barc170 and Xwmc718, from 320 pairs of SSR primer, on chromosome 4AL were found linked to the resistance gene (temporarily designated as YrLm2), the genetic distances of four markers to YrLm2 were 15.0, 5.0, 3.9, and 3.1cM, respectively. The dominant gene YrLm2 was located in the long leg of 4A chromosome, the results of markers can be used in wheat molecular-assisted breeding.

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