Abstract:In a greenhouse test conducted in 2007-2008, ten strain endophytic fungi isolated from cucumber seedlings have demonstrated good control efficacy to root-knot nematode Meloidogyne incognita. In order to elucidate their modes of action, split-root experiment design was used to investigate their ability to induce systemic resistance in cucumber against M.incognita. The roots of the same cucumber plant were divided into two isolated parts, one part was defined as challenging roots and another was defined as responding roots. With the inoculation of the Fusarium strains Fu234 and Fu654, Chaetomium sp. Ch1001 and Phyllosticta sp. Ph511 in the challenging roots, the nematodes penetration in the responding roots were significantly reduced, the reduction rate reached 42.4%, 35.7%, 38.4%, 23.6% in the first trail and 63.6%, 45.2%, 51.0%, 37.0% in the repeat trial, indicating that the four strains have induced resistance against invasion of M.incognita. With the inoculation of Fu234, Fu654, Ch1001 and Paecilomyces strain Pa972 in the challenging roots the ratio of eggs to females in the responding roots have significantly decreased, the reduction rate were 24.3%, 37.7%, 48.3%, 21.1% in the first trial and 21.6%, 39.8%, 30.0%, 46.2% in the second trial, indicating that these four strains endophytic fungi have induced resistance to nematode reproduction.