Abstract:Zhongliang93444 is highly resistant to three rusts of wheat. To identify and map the gene for stripe rust resistance, CYR30, CYR31 and CYR32 were selected to test Zhongliang93444, Mingxian169 and generations of Zhongling93444 crossed with Mingxian169. SSR technology was used to map the gene resistant to CYR32 based on F3-3 line. The results showed that the resistance in Zhongliang93444 to CYR30 and CYR31 were controlled by one dominant and one recessive gene, respectively. The resistance to CYR32, however, was controlled by two complementary dominant genes. The resistance of the family separation F3-3 of Zhongliang93444/Mingxian169 to CYR32 was controlled by one dominant gene which was temporarily designated as Yr93444. Eight SSR markers, Xgwm122, Xwmc702, Xwmc644, Xwmc794, Xgwm328, Xwmc455, Xgwm372, and Xwmc819, were linked to Yr93444, and the genetic distances were 38.1, 30.7, 22.9, 15.6, 10.0, 6.9, 3.5 and 2.8 cM, respectively. SSR markers, 21 nulli-tetrasomic and Chinese Spring lines located the gene on wheat chromosome 2AL. The pedigree analysis and molecular maker results indicated the origin of the gene was from Elytrigia intermedium. Yr93444 was detected in 89% of Zhongliang wheat cultivars by Xgwm372 and Xwmc819 as PCR primers, and was not detected in 86% of the 42 tested wheat cultivars from Huanghuai area with Xgwm372 and Xwmc819 as PCR primers. The results were useful for breeding resistance cultivars to stripe rust.