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期 刊 ：植物保护学报 2006年

关键词：丙环唑；斜纹夜蛾；细胞毒性；

Keywords:Propiconazole, Spodoptera litura, cytotoxicity,

摘 要 ：为从现有农药中寻找杀虫剂先导化合物,研究了11种农药对斜纹夜蛾细胞的毒杀活性。结果表明,杀菌剂丙环唑的活性最高,处理后24 h和48 h,丙环唑对斜纹夜蛾细胞的LC₅₀值分别为23.89 μg/mL和13.62 μg/mL。在活体试验中,以0.5 μg/头剂量的丙环唑注射斜纹夜蛾4龄幼虫,处理后24 h和48 h,试虫血淋巴中血细胞数分别下降了19.67%、21.38%,血淋巴含量分别下降了20.42%、23.87%。以0.5 μg/头和1.0 μg/头剂量的丙环唑注射处理后,斜纹夜蛾幼虫体重显著降低。在注射处理后48 h和72 h,丙环唑对斜纹夜蛾4龄幼虫的LD₅₀值分别为0.91 μg/头和0.63 μg/头。丙环唑对斜纹夜蛾细胞和斜纹夜蛾幼虫均具有较好的毒杀活性,显示出杀菌剂丙环唑衍生物控制害虫的可能性。

Abstract:In order to search lead compound of insecticide, cytotoxicity of 11 kinds of pesticides against Spodoptera litura cells were studied. The results showed that the cytotoxicity of propiconazole was the highest. The LC₅₀ values of propiconazole against S.litura cell were 23.89 μg/mL and 13.62 μg/mL after treated for 24 h and 48 h respectively. After 0.5 μg/larva propiconazole had been injected into the 4th instar larvae of S.litura for 24 h and 48 h, the reduced rates of blood corpuscle of larvae were 19.67% and 21.38%, and the reduced rates of blood content were 20.42% and 23.87% respectively. Furthermore, 0.5 μg/larva and 1.0 μg/larva propiconazole could depress the larvae weight of S.litura obviously. When treated with propiconazole for 48 h and 72 h, the LD₅₀ values of propiconazole against 4th instar larvae of S. litura were 0.91 μg/larva and 0.63 μg/larva respectively. It is firstly proved that propiconazole has a high toxicity against S. litura cell and S. litura larva in laboratory, which showed the possibility for propiconazole analog controlling insect pests.

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