Abstract:The complete coat protein gene of Scallion mosaic virus from wild scallion in Hangzhou City, Zhejiang Province was amplified by PCR with specific primers and then inserted into pSBET. The recombinant plasmid of pSB-ScaMV-CP was transformed to BL21plys S, in which the aimed protein was overexpressed after induced by IPTG and then purified by SDS-PAGE. The titre of antiserum obtained in the mice was 1∶1 024 assayed by indirect ELISA. Based on Western blot analysis, the polycloned antiserum obtained reacted strongly and specifically to its homologous protein. It also reacted less strongly to the prokaryotically expressed CPs of Turnip mosaic virus, Narcissus yellow stripe virus, Zucchini yellow mosaic virus, and Dasheen mosaic virus, but not to other 15 potyviruses.