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期 刊 ：植物保护学报 2005年

关键词：转cry1Ab基因水稻；花粉；中华草蛉；产卵；寿命；

Keywords:Transgenic cry1Ab rice, pollen, Chrysoperla sinica Tjeder, reproduction, longevity,

摘 要 ：用ELISA法测定了转Bt基因水稻克螟稻1号(KMD1)和克螟稻2号(KMD2)花粉中Cry1Ab含量。将转Bt基因水稻和其亲本对照秀水11(XS11)花粉加入到草蛉人工干粉饲料中,评价了对中华草蛉Chrysoperla sinica Tjeder成虫产卵和寿命等参数的影响。结果显示,转Bt基因水稻"克螟稻"花粉中表达Cry1Ab杀虫蛋白,且表达量较高。分别用含5 % KMD1、KMD2和XS11花粉的人工饲料饲喂中华草蛉初羽化成虫,发现两类Bt水稻花粉对该虫产卵前期、产卵量、产卵天数和成虫寿命等参数均无显著影响。人工饲料中水稻花粉的比例上升至20%和80%后,KMD1、KMD2和XS11花粉饲料处理中的草蛉均出现产卵前期延长、产卵量减少和产卵天数缩短的趋势,但在同一花粉比例下这些参数在3个花粉饲料处理间均无显著差异。结果表明,Bt水稻花粉中Cry1Ab杀虫蛋白对该草蛉成虫产卵和寿命等参数无显著的负面影响。

Abstract:The concentrations of Cry1Ab insecticidal protein expressed in pollens of transgenic Bt rice KMD1 and KMD2 from a commercial Chinese japonica rice variety Xiushui 11 (XS11), contain a synthetic cry1Ab gene from the bacterium Bacillus thuringiensis (Bt) Berliner, were detected by enzyme immunosorbent assay (ELISA) with the Quantiplate^TM kit for Bt Cry1Ab protein. The results showed that the pollens of KMD1 and KMD2 can express Cry1Ab protein highly. Newly emerged Chrysoperla sinica Tjeder adults were provided with three different artificial diets (dry yeast powder-sugar mixture) which contained 5% pollens of KMD1, KMD2 and the control rice line XS11, respectively, and their preovipositional period, fecundity, ovipositional days and longevity were observed. The results showed that none of these parameters differed significantly among the three pollen treatments, even if the proportion of pollen in the artificial diets increased to 20% and 80%. This indicated that the insecticidal protein Cry1Ab in pollens of KMD1 and KMD2 had no significant negative effects on the reproduction and adult longevity of C.sinica.

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