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期 刊 ：植物保护学报 2007年

关键词：茶毛虫核型多角体病毒；苏云金杆菌；混剂；增效作用；

Keywords:Euproctis pseudoconspersa nuclear polyhedrosis virus, Bacillus thuringiensis, mixture, synergism effect,

摘 要 ：用生物测定的方法,研究了茶毛虫核型多角体病毒(EpNPV)与苏云金杆菌(Bt)配制而成的茶毛虫核型多角体病毒Bt混剂(EpNPV-Bt)的增效作用、速效作用、拒食作用和兼治作用。结果表明,在16~28℃温度范围内,EpNPV-Bt对茶毛虫的作用在整个死亡周期表现为相加作用,协同毒力指数主要分布在－20％~20％之间, 其中16、19、28℃的死亡前期表现为增效作用,协同毒力指数大于20％,22℃的死亡中期表现为拮抗作用,协同毒力指数小于－20％。 EpNPV-Bt对茶毛虫LT₃₀为5.6天,比EpNPV减少3.8天,幼虫的死亡速度提高了1.8倍。EpNPV-Bt对茶毛虫的拒食效果明显,取食量比EpNPV减少66.2％。EpNPV-Bt对茶刺蛾、用克尺蠖和茶尺蠖等茶树害虫具有兼治作用,其中对茶刺蛾的死亡率达85.8%。

Abstract:Bioassay were conducted to study the synergism, immediate functioning, anti-feeding effect and dual effect of EpNPV-Bt, the mixture of Euproctis pseudoconspersa Strand nuclear polyhedrosis virus (EpNPV) and Bacillus thuringiensis (Bt). Under the temperature ranged from 16℃ to 28℃, the results showed that the EpNPV-Bt against the larvae of Euproctis pseudoconspersa Strand possessed joint action at the whole death period, the dynamic of synergic toxicity was mostly distributed between －20% to 20%. Hereinto at the death initial period of the 16℃, 19℃ and 28℃ treatments, the dynamic of synergic toxicity was more than 20% which showed synergism. At the death middle period of 22℃ treatment, the dynamic of synergic toxicity was less than -20%, which showed antagonism. Compared with EpNPV, the immediate functioning and anti-feeding effect of the EpNPV-Bt was evident. The LT₃₀ (30% lethal time) of the EpNPV-Bt against the larvae of Euproctis pseudoconspersa Strand was lessened from 9.4d to 5.6d, and the food intake was decreased to 66.2%. It also showed a good dual effect of the EpNPV-Bt to control the larvae of Iragoides fasciata (Moore), Jankowskia athleta Oberthur and Ectropis oblique Warren which are the main tea pests. The mortality of the EpNPV-Bt against the larvae of Iragoides fasciata (Moore) was up to 85.8%.

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