Abstract:Bioassay of four Escherichia coli of experessing Bacillus thuringiensis δ-endotoxin gene against forest pests (Lymantria dispar, Apocheimacinerarius, Dendrolimus punctatus, Clostera anachoreta) showed: The four cloned strains had high toxicity against above-mentioned pests and could inhibit growth and development of the pests.