Abstract:A strain of actinomycetes E2, which was isolated from the rhiszosphere of diseased pepper, was primarily identified as Micromonospora sp. based on morphological and cultural traits. A study was carried out on the inhibiting effects of the fermented broth and extracts of E2’ s mycelia.The results showed that E2 could inhibit the growth of various fungi and bacteria and the inhibition was stable.The control effect of E2 to Phytophthora blight of pepper seedling could reach 75% -85%.By means of antibiotic special identification technique including the paper chromatography of eight kings of solvent system and characteristic chemical reaction, E2 was also indicated to produce polyene-like substances.