作 者 ：王巧兰1;2;吴礼树1;赵竹青1;赵林萍3;Derrick Oosterhuis4
期 刊 ：植物营养与肥料学报 2005年 11卷 5期 页码：665-669
Keywords:cotton, nitrogen status, estimate, SPAD meter, Cardy meter,
摘 要 ：在田间和温室条件下分别用传统的硝化测氮法与叶绿素仪(SPAD.meter)和Cardy.NO3-nitrate.meter两种便携式氮素测定仪对棉花花铃期氮素营养状况进行了评估和跟踪。结果表明,传统的硝化测氮法测得的叶片全N和叶柄NO3--N含量和叶绿素仪(SPAD.meter)及Cardy.NO3-nitrate.meter的读数都反应出大田生长的棉株叶片和叶柄的含氮量因施氮水平、棉桃载荷和后期施用叶面氮肥而不同。在较高施氮水平下,单株棉桃数较少(即低载荷)以及叶面施用氮肥的棉株叶片和叶柄的含氮量明显高于其它处理,且叶绿素仪(SPAD.meter)和Cardy.NO3-nitrate.meter的读数与叶片的全N百分数和叶柄NO3--N含量存在显著的正相关,其相关系数在大田条件下平均为0.70;温室条件下平均为0.90。
Abstract:Field and greenhouse experiments were conducted at the Arkansas Agricultural Research and Extension Center in Fayetteville with the objective of comparing conventional leaf-blade and petiole N combustion methods with SPAD meter ( Minolta Chlorophyll Meter ) and Cardy meter (Horiba, Ltd., Kyoto, Japan) to assess and monitor the nitrogen status at bloom stage of cotton. The layout of field trial was a split-split-plot design with six replications. The main plots consisted of soil fertilizer N levels, high N (HN) and low N (LN), the sub plots were high (HB) and low (LB) boll load, and the sub-sub-plots consisted of foliar and no-foliar N applications. Tissue samples were collected with a week(7 days) interval starting from a week after first flower and keeping for five weeks. Correlation analyses was used to compare chlorophyll meter readings, measured with the SPAD meter with the leaf-blade N concentration, measured using the traditional laboratory analytical method as well as NO3--N concentrations of cotton petiole sap, measured by the Cardy meter, and traditional laboratory analytical method respectively. This study showed that chlorophyll meter readings measured with the SPAD meter and Cardy meter reading were significantly correlated with leaf-blade N concentration and petiole NO3--N. The reading of these approaches indicated N content in leaf-blade and leaf stem varying along with the change of N addressing rate, boll load and foliar fertilizer applied at anaphase stage. At high N addressing rate, N content in leaf blade and leaf stem of the cotton with low ball load or foliar fertilizer application was significantly higher than others. The results also showed that the hand-held chlorophyll meter and Cardy meter might be as reliable as leaf-blade N and petiole NO3--N for the purpose of supplemental N fertilizer requirements predication of cotton. They were promising valuable tools to monitor the in-season N status of irrigated cotton in Arkansas. Further field comparisons of these methods under different environmental conditions, i.e. drought, should be carried out in the future.
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