作 者 ：胡炳义1;2;牛明功2;王启明1;李潮海1;刘怀攀1;2
期 刊 ：植物营养与肥料学报 2006年 12卷 6期 页码：881-886
Keywords:osmotic stress, polyamine forms, soybean (Glycine max L.),
摘 要 ：用高效液相色谱(HPLC)法对渗透胁迫下两个抗旱性不同的大豆品种(抗旱性弱的豫豆6号和抗旱性强的豫豆24号)幼苗叶片的三种不同形态多胺游离态(Free:f)、酸可溶性共价结合态(Acid.soluble.covalently.conjugated:AS-CC)和酸不溶性共价结合态(Acid.insoluble.covalently.conjugated:AISCC))的含量变化进行了研究。结果如下:三种游离态多胺:腐胺(fPut)、亚精胺(fSpd)和精胺(fSpm)均在渗透胁迫条件下上升,但是豫豆24号的fSpd和fSpm的上升幅度明显大于豫豆6号,而豫豆6号的fPut的升幅明显大于豫豆24号。甲基乙二醛-双(鸟嘌呤腙)(MGBG)处理豫豆24,明显抑制了渗透胁迫诱导的fSpd和fSpm的增加,并且加重了渗透胁迫的伤害;外源Spd处理豫豆6号明显促进了渗透胁迫诱导的fSpd和fSpm的增加,并且减缓了渗透胁迫的伤害。统计分析表明:在渗透胁迫条件下,大豆幼苗叶片的fSpd+fSpm/fPut的比值与相对干重增长率(RDWIR)呈显著正相关。渗透胁迫下,豫豆24叶片的AISCC-PAs含量的上升幅度明显大于豫豆6号。菲咯啉处理明显抑制了渗透胁迫所诱导AISCC-PAs的增加,同时也加重了渗透胁迫对幼苗的伤害。渗透胁迫也引起了幼苗叶片中ASCC-PAs含量的上升,但是在两个大豆品种之间的上升幅度没有差异。这些结果表明,渗透胁迫条件下,大豆幼苗叶片的fSpd、fSpm及AISCC-PAs含量的上升有利于增强大豆幼苗的抗胁迫能力。
Abstract:The changes in content and form of polyamines(PAs) in the leaves of two soybean (Glycine max L.) cultivar seedlings differing in drought tolerance were investigated with HPLC under osmotic stress.Yudou No.24 cv.(drought-tolerant) showed higher increases magnitude in free-spermidine(fSpd) and free-spermine(fSpm) in leaves than Yudou No.6 cv.(drought-sensitive) after treated with polyethylene glycol(PEG) 6,000 for 7 d,while Yudou No.6 cv.showed a higher increased magnitude in free putrescine(fPut).Thus Yudou No.24 cv.gave a higher ratio of(fSpd + fSpm) / fPut than Yudou No.6 cv.in leaves under osmotic stress.Exogenous treatment of seedlings with methylglyoxal-bis(guanylhydrazone)(MGBG),an S-adenosylmethionine decarboxylase(SAMDC,which catalyzes the Spd and Sam biosynthesis) inhibitor,resulted in a reduction of fSpd and fSpm in leaves and aggravation of PEG-induced injury to Yudou No.24 cv.seedlings,while exogenous Spd treatment resulted in an increase in fSpd + fSpm in leaves,furthermore retarded PEG-induced injury in Yudou No.6 cv.seedling.There was no statistically significant difference between the contents of acid soluble covalently conjugated PAs(ASCC-PAs) in leaves of both cultivars under PEG osmotic stress,suggesting that the level of ASCC-PAs in leaves had no relationship with the osmotic stress tolerance of wheat seedlings.Osmotic stress induced a significant increase in acid insoluble covalently conjugated polyamines(AISCC-PAs) level in seedling leaves of Yudou No.24 cv.,whereas it had a slightly influence on AISCC-PAs in Yudou No.6 cv.seedlings.Exogenous treatment of seedlings with phenanthrolin(o-Phen),an inhibitor of transglutaminase(TGase,which catalyzes AISCC-PAs biosynthesis),also aggravated PEG-induced injury to Yudou No.24 cv.seedlings,combined with a decrease in AISCC-PAs levels.The results suggested that fSpd,fSpm and PIS-Pas,which strengthened the osmotic stress tolerant capacity of soybean seedlings.
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