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作 者 ：陈济琛;林新坚;韩闽毅;郑时利;刘中柱

期 刊 ：植物营养与肥料学报 1996年 2卷 1期 页码：74-78

关键词：脲酶；脲酶抑制剂；I36N；赭曲霉；

摘 要 ：利用一株赭曲霉代谢产物I₃₆N作为脲酶抑制剂研究对土壤脲酶活性的抑制作用,结果表明,I₃₆N对我省主要5种水稻田土壤脲酶活性具有明显的抑制作用。土壤脲酶活性高,I₃₆N抑制作用强,但抑制时间较短。抑制活性随I₃₆N浓度而增强。与化学抑制剂NBPT相比,虽然I₃₆N对土壤脲酶活性的抑制所需用量较大,但抑制时间延长。盆栽试验结果表明,施加I₃₆N后,水层尿素水解速率降低,(NH₄⁺+NH₃)—N高峰期比对照推迟2～4天,高峰值也较低。水层pH值变化规律与(NH₄⁺+NH₃)—N变化一致。

Abstract:The investigation results showed that the metabolic product I₃₆N from Aspergilius sp. has an obvious inhibition to urease activity of five main kinds of soil in our province. The rate of I₃₆N inhibition on soil urease varied with the activity of soil urease and its effect on higher activity of soil urease is better than its on lower, but was prolonged in the period of retarding urease activity. The inhibiting degree was increased with the increase of I₃₆N application amount and urea concentration used. The pot experiment indicated that I36N could postponed effectively the hydrolysis of urea, delayed high peak of (NH₄⁺+NH₃)-N from 2 to 4 days and the level of NH₃ concentration was lower than control, the change of the pH value was consilience with behaviour of (NH₄⁺+NH₃)-N in the pot water.

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