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Effect of NaCl Stress on the Leaf Photosynthesis and Chlorophyll Fluorescence Parameters of Daphne giraldii Nitsche

NaCl胁迫对祁连山野生黄瑞香叶片光合叶绿素荧光特性的影响



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,2016,36(6):1182-1189
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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犌s«—˜¬­¹£º,»¼½CO2 uv(犆i)«—˜¬­¹<¾。(2)¿uv(50mmol·L-1)NaClwx¤j
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犉犾狌狅狉犲狊犮犲狀犮犲犘犪狉犪犿犲狋犲狉狊狅犳犇犪狆犺狀犲犵犻狉犪犾犱犻 犖犻狋狊犮犺犲
YANFang1,2,WANGQinli1,2,GUOYouyan2,ZHANGYajuan2,HOULiangyu2
(1KeyLaboratoryofHexiCorridorResourcesUtilizationofGansuUniversity,Zhangye,Gansu734000,China;2ResearchCen
terofVegetableEngineeringandTechnologyofHexiUniversity,Zhangye,Gansu734000,China)
犃犫狊狋狉犪犮狋:Withfourtrueleavesof犇犪狆犺狀犲犵犻狉犪犾犱犻犻Nitscheasthematerial,weinvestigatedtheeffectsof
variousNaClstress(50,100,150and200mmol·L-1)onphotosyntheticphysiologicalindexesasseedling
photosyntheticpigmentcontent,chlorophylfluorescenceparametersandgasexchangeparametersof犇.
犵犻狉犪犾犱犻犻Nitsche.basedonthepotsexperimentsinsandculturesystematicaly.Theresultshowedthat:
(1)Underaproperenvironmentalcondition(Control),thedailyvariationof犘nand犌sarebothilustrated
bydoublepeakcurvewhilethedailyvariationof犜risrepresentedbyasinglepeakone,thusthedailyvari
ationof犇.犵犻狉犪犾犱犻犻Nitsche.’sleavesischangedbyahigherconcentratedNaClstress(>100mmol·L-1)
thatcausesanoveraldeclinetothedailyvariationcurvesof犘n,犜r,aswelas犌s,andanoveralriseof
犆i’sdailyvariationcurve;(2)AlowerconcentratedNaClstress(50mmol·L-1)haslittleeffectsonthe
leafchlorophylcontentandtheratioof犇.犵犻狉犪犾犱犻犻Nitscheanditschlorophylcontentdeclinedsignifi
cantlybyahigherconcentratedNaClstress(>100mmol·L-1)whentheratiohadaslightdecrease.(3)
犉m,犉v/犉m,犉v′/犉m′,狇P,ΦPSⅡarealdeclinedsignificantlybyahigherconcentratedNaClstress(>100
mmol·L-1),while犉0andNPQarebothraisedsignificantly.Theresearchshowsthatwiththeincreasing
ofNaClconcentration,thereasonforleadingtothedecreaseofphotosyntheticratewasmainlycausedby
thenostomatalfactors;犇.犵犻狉犪犾犱犻犻NitscheisfairlyresistibleoflessNaClstressbutaworseNaClstress
maydamagetheactivityofphotosyntheticapparatus,therebysurgingphotoinhibitionandrestrictingpho
tosynthesis.
犓犲狔狑狅狉犱狊:犇犪狆犺狀犲犵犻狉犪犾犱犻犻;NaClstress;chlorophylfluorescence;photosynthesis
  jkl(犇犪狆犺狀犲犵犻狉犪犾犱犻犻Nitsche)oklAk
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1.2.3 ?CDEAij L>10:00þÿ?Š“
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min,¥-HþŠÜþÌÖ׉Š(犉0),L犉0 …Ü
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ãÖMÃNQAOÑyz’ãPG,þÌÍΉ
ŠÁ
(犉m),µ;<û—‰Š 犉v(犉v= 犉m- 犉0)。
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†K½–‰ŠÁ

L^àó4Š–tK4IØJK
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-犉0′)/犉m′
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-犉0′)
PSⅡ8ӊ˜%ϨΦPSⅡ= (犉m′-犉s)/犉m′
/1 2015(7â18[Z[Ɗ“çÏ,-
Øig|v«—˜
Fig.1 Diurnalfluctuationsofphotosyntheticalyactive
radiation(犘犃犚)andleaftemperatureinfulsunlight
onJuly18,2015inZhangye
  ي˜%ÑÒŒ犖犘犙=犉m/犉m′-1
1.3 jmno
Â9Œ\34 Origin8.5]/,{4 SPSS
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(a+b)]、i…†a(Chla)Øi…†b(Chlb)‡ˆL
¿vÞwx
(50mmol·L-1)£ÔY¤¥(CK)ÂÃ
Äcd
(犘>0.05);i…†‡ˆLVvÞwx(100
~150mmol·L-1)KiÀ¤¥ÃÄ£º(犘<
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(200mmol·L-1)KºôÍ¿
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(犘<0.05)。
tK

i…†aYi…†b–Àa(Chla/Chlb)Ü
Ýýi…†‡ˆ–—˜»¶—

NChlaØChlb
LýÞwx¡¢£st£ºbv–ÅÆ
,Chla/Chl
bgLV6vÞwxyz(150~200mmol·L-1)
ÃÄõz¤¥
(犘<0.05),»150Ø200mmol·
L-1uvyz½ÂÃÄcd;h@jklmnig
i…†t¤‡ˆLÞwx£i®œÃjklm

Ç
Chla/ChlbÁ—˜t¤ÊË,AÉL6vÞwx¡
¢£5ŒH—t¤Vÿ

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IJ
2.2.1 uABvw(犘狀)st stuvÞwx
¤jklmn¦Š“§¨
(犘n)«—˜ÅƵÎ(/
x1 p45y<=>?CDklz{|Hst
Table1 Thechlorophylcontentanditsproportioninleavesof犇犪狆犺狀犲犵犻狉犪犾犱犻犻seedlingunderNaClstress/(mg·g-1)
Þuv
NaClconcentration
i…†a
Chlorophyla
i…†b
Chlorophylb
i…†
(a+b)
Chl(a+b)
i…†a/i…†b
Chla/Chlb
0(CK) 1.440±0.214a 0.410±0.025a 1.850±0.024a 3.510±0.027a
50 1.460±0.032a 0.437±0.041a 1.897±0.035a 3.341±0.036a
100 0.745±0.043b 0.223±0.045b 0.968±0.063c 3.340±0.055a
150 0.774±0.031b 0.263±0.052ab 1.037±0.071bc 2.940±0.046b
200 0.425±0.062c 0.157±0.048b 0.582±0.028d 2.710±0.056b
n

›VþÿÁÔf>eˆoBv

Œ\o3ñþÿÔÁ±•_`;tlVstop›1yz½cdüU0.05ÃĖÊ。
Note:Thevaluesinthetableweretestedbyfreshweight,andthedatawere3timesofthemeanvalue±standarderror,Differentletters
inthesamecolumnindicatesignificantdifferencesamongtreatmentsat0.05level.
4811 ! " # $ % &                   36q
/2 stuvÞwx£jklig犘n、犌s、犆iØ犜r–«—˜¬­
Fig.2 Diurnalchangesof犘n,犌s,犆iand犜rin犇.犵犻狉犪犾犱犻犻leavesunderdifferentNaClstresstreatments
2,A)。„V,¤¥ig犘n–«—˜®¯°¬­,ë
1áµ*°Á¤ÛL12:00,ë2ᵿ–°Á¤Û
L16:00。jklig–犘n ÁLýuv–Þyz
£Ôstbv+£º

DŽL¿Þwxyz£Y¤
¥cdsÎ

»ÜÝÞuv–«†r¾*
,犘n £º
–svfgfÎ

°Áº¿»¤ÛK½¾n

t„å
150~200mmol·L-1NaClyzÉÌig–Š“u
vw¤¥ØV¿uvyz–14:00¾nô12:00。
2.2.2 e}~(犌狊)st ./2,B01,L
ÂÞwx¡¢

¤¥

£

jklmn–Ž©ªv
(犌s)«—˜lmY犘n t†¸,„ig–犌s«—˜
®¯°¬­

Í*Á¤ÛL12:00,8:00Y18:00
–ÁtcsÎ

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jk–xy5Yt.–犘n —˜‡zt{,/öå
NaCluvo50mmol·L-1K–犌sÁY¤¥cds
Î

ÜÝÞwxbv–àî

mn犌sÁ®£ºlm,
£ºsvÜÝÞuv–ßλßÎ

6vÞyz¡
¢£犌sÁ£ºsvÍÎ,„?12:00üU°Á܆
|º¿

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),
jkl–¼½CO2 uv
(犆i)z10:00£ºôÍ¿Á,»L14:00üÍ*Á。
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Ûo¿vØVvÞwx£犆iÁõsv*z¤¥,Ç
L6vÞwx£犆iÁ<¾svµÎ,¿Þwx–
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x犆iÁ–°ÁØÍ¿Á¤Û–K½7—˜µo†
¸

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(犜r)O«
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Í*Á¤ÛL12:00
~14:00€;ýuvÞwx¡¢£jklig–
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Σºsv‚ƒßÎ

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{

t„…½cd5sœÃ

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ig犜rÁ–«—˜lzÊˍ°Ás.¤¥œÃ。
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£jklRSŠ“ó4‹ŒYi…†”•«¬tu
ý^™š

›2)›œ,ig犘nY犌sØ犜rÔ®…Ã
ĝtu
(犘<0.01),»Yi…†”•tusÃÄ
(犘>0.05);犆iY犘n®…ÃĆtu,»Y犌s、犜r
Ô®ÃĆtu
(犘<0.05),Yi…†”•5®†
tuÇsÃÄ

‡d†tuˆœjklmn¦Š“
§¨ÜŠèßà»ß*åwz„i‰Š¼Š“í·
ßΖ™š

»Ù犆iº¿–™š,ÇsŒw‡d†
tuŸÌ¤犆if¿ÈŠ“§¨f*–™´。ˆœ
LÞwx¡¢£

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¿ûŒåNU„igŽ©–u‹¼½CO2 uv
–ÅÆ

»YŠ“0†–‡ˆusÎ

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2.3 p456<=>qr?CDEAijHIJ
i…†‰Š‹ŒŒL†ÿbv•Ñ†l6S–Ž·b
[17]。犉0(Ö׉Š)、
犉m(ÍΉŠ)ýö”Š€Ⅱ(PSⅡ)Y.Vey
z?OJXØ?Ou‹K–‰Š–Ê

»犉0 –¾
*YPSⅡY.Ve–D1 ‘íçu[18]。„V,
jklmnNUstbvÞwxÜ

„igF0 Ôç
0ßà

tf6vÞwxßàsvÍÎ

»VvÞy
z½cdsÃÄ

ÇýÞwxyzY¤¥½ÔüU
ÃÄcd–Ê
(犘<0.05),‡ˆœÞwxÉPSⅡY
.Veû’‘í¯N“

/3,A);tK,ÜÞwxu
v–¾*

jklmnig犉m Á®£ºlm,tf
¿vÞwx£ºsvÍÎ

ýÞuvyzY¤¥
½cdÔüUƒÃĖÊ
(犘<0.05),›œPSⅡY
.Ve–MÔjŒ•L¿ÞwxKiœÃk–

/3,B)。
—Ú
,犉v(û—‰Š)oFm YF0 –cÁ,Q›
ãÖMÃN
(QA)–’ãPG[19];»犉v/犉0、犉v/
犉m ÈÔûYŒPSⅡÍΈÃϨ。/3,C、DÃ
1

ÜÝÞwxuv–¾*

jklmnig犉v/犉0
Ø犉v/犉m ÁÔç0kõ,ýuvyzY¤¥tÀ
Ô®ÃÄ£ºlm
(犘<0.05)。‡ˆœjklmn
igPSⅡY.VeiäÞwx»NUƒ˜™,„š
Lí·Øã֊ŒZ‘ϨœÃk–

ä›#$
•ÚQƒŠ“ó4–Šïð

Y›tK

ýuv NaClwx¤jkligPS
Ⅱ8ӊ˜%Ϩ(ΦPSⅡ)、JX–PSⅡY.VeÐ
œLڌZ˜Ï¨
(犉v′/犉m′)、Š˜‰ŠÑÒŒ
(狇P)ØYŒ7U—˜–ÙŠ˜‰ŠÑÒŒ
(犖犘犙)Ô@çÃÄ·ÅÆ(/4)。„V,犉v′/犉m′å
x2 <=>†LABij\?CDaUH‡Mˆ‰
Table2 Correlationmatrixbetweenmainphotosyntheticparametersandchlorophylindexesof犇犪狆犺狀犲犵犻狉犪犾犱犻犻leaves
‹ŒParameter 犘n 犌s 犆i 犜r Chla Chlb Chl(a+b) Chla/Chlb
犘n 1.000 0.963 -0.954 0.887 0.834 0.827 0.844 0.369
犌s 1.000 -0.723 0.820 0.829 0.836 0.826 0.722
犆i 1.000 -0.894 -0.725 -0.794 -0.747 -0.508
犜r 1.000 0.890 0.870 0.894 0.835
Chla 1.000 0.978 0.999 0.796
Chlb 1.000 0.986 0.668
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