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,2016,36(6):1182-1189
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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K L:f4ghijklmnopq,]rstuv(0、50、100、150、200mmol·L-1)NaClwxyz,{4|:
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:(1)
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jklig¦§¨
(犘n)、©ªv(犌s)«¬®¯°±,²³§¨(犜r)«
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µ*uv
(>100mmol·L-1)NaClwx¶jklig/·«¬,ª¸犘n、犜r、
犌s«¬¹£º,»¼½CO2 uv(犆i)«¬¹<¾。(2)¿uv(50mmol·L-1)NaClwx¤j
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(犉v/犉m)、PSⅡ£ÍÎÐϨ(犉v′/犉m′)、%ÑÒ(狇P)、PSⅡ8Ó%Ϩ(ΦPSⅡ)ÔÃ
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犈犳犳犲犮狋狅犳犖犪犆犾犛狋狉犲狊狊狅狀狋犺犲犔犲犪犳犘犺狅狋狅狊狔狀狋犺犲狊犻狊犪狀犱犆犺犾狅狉狅狆犺狔犾
犉犾狌狅狉犲狊犮犲狀犮犲犘犪狉犪犿犲狋犲狉狊狅犳犇犪狆犺狀犲犵犻狉犪犾犱犻 犖犻狋狊犮犺犲
YANFang1,2,WANGQinli1,2,GUOYouyan2,ZHANGYajuan2,HOULiangyu2
(1KeyLaboratoryofHexiCorridorResourcesUtilizationofGansuUniversity,Zhangye,Gansu734000,China;2ResearchCen
terofVegetableEngineeringandTechnologyofHexiUniversity,Zhangye,Gansu734000,China)
犃犫狊狋狉犪犮狋:Withfourtrueleavesof犇犪狆犺狀犲犵犻狉犪犾犱犻犻Nitscheasthematerial,weinvestigatedtheeffectsof
variousNaClstress(50,100,150and200mmol·L-1)onphotosyntheticphysiologicalindexesasseedling
photosyntheticpigmentcontent,chlorophylfluorescenceparametersandgasexchangeparametersof犇.
犵犻狉犪犾犱犻犻Nitsche.basedonthepotsexperimentsinsandculturesystematicaly.Theresultshowedthat:
(1)Underaproperenvironmentalcondition(Control),thedailyvariationof犘nand犌sarebothilustrated
bydoublepeakcurvewhilethedailyvariationof犜risrepresentedbyasinglepeakone,thusthedailyvari
ationof犇.犵犻狉犪犾犱犻犻Nitsche.’sleavesischangedbyahigherconcentratedNaClstress(>100mmol·L-1)
thatcausesanoveraldeclinetothedailyvariationcurvesof犘n,犜r,aswelas犌s,andanoveralriseof
犆i’sdailyvariationcurve;(2)AlowerconcentratedNaClstress(50mmol·L-1)haslittleeffectsonthe
leafchlorophylcontentandtheratioof犇.犵犻狉犪犾犱犻犻Nitscheanditschlorophylcontentdeclinedsignifi
cantlybyahigherconcentratedNaClstress(>100mmol·L-1)whentheratiohadaslightdecrease.(3)
犉m,犉v/犉m,犉v′/犉m′,狇P,ΦPSⅡarealdeclinedsignificantlybyahigherconcentratedNaClstress(>100
mmol·L-1),while犉0andNPQarebothraisedsignificantly.Theresearchshowsthatwiththeincreasing
ofNaClconcentration,thereasonforleadingtothedecreaseofphotosyntheticratewasmainlycausedby
thenostomatalfactors;犇.犵犻狉犪犾犱犻犻NitscheisfairlyresistibleoflessNaClstressbutaworseNaClstress
maydamagetheactivityofphotosyntheticapparatus,therebysurgingphotoinhibitionandrestrictingpho
tosynthesis.
犓犲狔狑狅狉犱狊:犇犪狆犺狀犲犵犻狉犪犾犱犻犻;NaClstress;chlorophylfluorescence;photosynthesis
jkl(犇犪狆犺狀犲犵犻狉犪犾犱犻犻Nitsche)oklAk
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ýþLVÿ(
、
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ð3ñ,ï¸3ø。{41/2Hoagland~êòóö,
|:?@¥
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~êô4ghiKJ×LõêòV
à_NaCl«¬wxyz,uvýöo50、100、150、
200mmol·L-1,fsàNaCl÷ò1/2Hoagland
õêòóo¤¥
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ön4ÙøÃùýúûçü}Be
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{4üÿCIRAS2±þÿ$¤ïáy
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Â9z2015(7â18«8:00~18:00«
¬
,
ï)2hþÿ1*,þ̦§¨(犘n)、©
ªv
(犌s)、¼½CO2 uv(犆i)、²³§¨(犜r)«
。
þÿ+Ç
(7â18«)()Z[Æç
Ï,-Øig|v./101。
1.2.2 ?CDkl i
{423Ø45
Ûò×¾Á¾6
,
4ývÁþÿ×¾òL
645Ø663nmy7vÁ犃645Ø犃663,8Licht
enchaler9:;(mg·g-1)。pq6ïáyzýôst#ø%
Ñ3giÃ。
38116= D E,:NaClwx¤ÊË$cQjkligi
/·ÅÆ
1.2.3 ?CDEAij L>10:00þÿ?
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,
@A4BC:$
(Hand
yPEA,üÿ Hansatech9D)¤ttig«¬i
þÿ
。
þÿnEFÖigG-.30
min,¥-HþÜþÌÖ×(犉0),L犉0
Ü
4èIØJK
(3000μmol·m
-2·s-1)LÚ,É
ãÖMÃNQAOÑyzãPG,þÌÍÎ
Á
(犉m),µ;<û 犉v(犉v= 犉m- 犉0)。
犉v/犉mQPSⅡÍÎÃϨ,犉1ó4£R
K½Á
。
L^àó4tK4IØJK
S
,
É犙A yzTKÍÎãPG,ÌUÁ
犉m′ØVG犉s,Wi
;<9:
.£
:
PSⅡJXY.VeZϨ犉v′/犉m′= (犉m′
-犉0′)/犉m′
ÑÒ狇P= (犉m′- 犉s)/(犉m′
-犉0′)
PSⅡ8Ó%ϨΦPSⅡ= (犉m′-犉s)/犉m′
/1 2015(7â18[Z[ÆçÏ,-
Øig|v«
Fig.1 Diurnalfluctuationsofphotosyntheticalyactive
radiation(犘犃犚)andleaftemperatureinfulsunlight
onJuly18,2015inZhangye
Ù%ÑÒ犖犘犙=犉m/犉m′-1
1.3 jmno
Â9\34 Origin8.5]/,{4 SPSS
19.0«¬;ý^,;<ýyz·PÊÔÁ、_
`
,
µ«¬tu·ý^
。
2 Yý^
2.1 p456<=>qr?CDklHIJ
ÚÑ ¶ûfáâ#$igi
ØÀa
,
«»áâ#$¶
[16]。
1Ã1,jklmnigi
b[Chl
(a+b)]、i
a(Chla)Øi
b(Chlb)L
¿vÞwx
(50mmol·L-1)£ÔY¤¥(CK)ÂÃ
Äcd
(犘>0.05);i
LVvÞwx(100
~150mmol·L-1)KiÀ¤¥ÃÄ£º(犘<
0.05),ÇVvÞwxyz
½eçÃÄcd;i
L6vÞwx
(200mmol·L-1)KºôÍ¿
Á
,
ÃÄ¿z¤¥ØfÞwxyz
(犘<0.05)。
tK
,
i
aYi
bÀa(Chla/Chlb)Ü
Ýýi
Ȧ
。
NChlaØChlb
LýÞwx¡¢£st£ºbvÅÆ
,Chla/Chl
bgLV6vÞwxyz(150~200mmol·L-1)
ÃÄõz¤¥
(犘<0.05),»150Ø200mmol·
L-1uvyz½ÂÃÄcd;h@jklmnig
i
t¤LÞwx£i®Ãjklm
,
Ç
Chla/ChlbÁt¤ÊË,AÉL6vÞwx¡
¢£5Ht¤Vÿ
。
2.2 p456<=>qrAB0dijstH
IJ
2.2.1 uABvw(犘狀)st stuvÞwx
¤jklmn¦§¨
(犘n)«ÅƵÎ(/
x1 p45y<=>?CDklz{|Hst
Table1 Thechlorophylcontentanditsproportioninleavesof犇犪狆犺狀犲犵犻狉犪犾犱犻犻seedlingunderNaClstress/(mg·g-1)
Þuv
NaClconcentration
i
a
Chlorophyla
i
b
Chlorophylb
i
(a+b)
Chl(a+b)
i
a/i
b
Chla/Chlb
0(CK) 1.440±0.214a 0.410±0.025a 1.850±0.024a 3.510±0.027a
50 1.460±0.032a 0.437±0.041a 1.897±0.035a 3.341±0.036a
100 0.745±0.043b 0.223±0.045b 0.968±0.063c 3.340±0.055a
150 0.774±0.031b 0.263±0.052ab 1.037±0.071bc 2.940±0.046b
200 0.425±0.062c 0.157±0.048b 0.582±0.028d 2.710±0.056b
n
:
VþÿÁÔf>eoBv
,
\o3ñþÿÔÁ±_`;tlVstop1yz½cdüU0.05ÃÄÊ。
Note:Thevaluesinthetableweretestedbyfreshweight,andthedatawere3timesofthemeanvalue±standarderror,Differentletters
inthesamecolumnindicatesignificantdifferencesamongtreatmentsat0.05level.
4811 ! " # $ % & 36q
/2 stuvÞwx£jklig犘n、犌s、犆iØ犜r«¬
Fig.2 Diurnalchangesof犘n,犌s,犆iand犜rin犇.犵犻狉犪犾犱犻犻leavesunderdifferentNaClstresstreatments
2,A)。V,¤¥ig犘n«®¯°¬,ë
1áµ*°Á¤ÛL12:00,ë2ᵿ°Á¤Û
L16:00。jklig犘n ÁLýuvÞyz
£Ôstbv+£º
,
ÇL¿Þwxyz£Y¤
¥cdsÎ
,
»ÜÝÞuv«r¾*
,犘n £º
svfgfÎ
,
°Áº¿»¤ÛK½¾n
,
tå
150~200mmol·L-1NaClyzÉÌigu
vw¤¥ØV¿uvyz14:00¾nô12:00。
2.2.2 e}~(犌狊)st ./2,B01,L
ÂÞwx¡¢
(
¤¥
)
£
,
jklmn©ªv
(犌s)«lmY犘n t¸,ig犌s«
®¯°¬
,
Í*Á¤ÛL12:00,8:00Y18:00
ÁtcsÎ
。
¿uvÞwxyz犌s Y¤¥tÀ
jkxy5Yt.犘n zt{,/öå
NaCluvo50mmol·L-1K犌sÁY¤¥cds
Î
;
ÜÝÞwxbvàî
,
mn犌sÁ®£ºlm,
£ºsvÜÝÞuvßλßÎ
;
6vÞyz¡
¢£犌sÁ£ºsvÍÎ,?12:00üU°ÁÜ
|º¿
,
«¬5ÚQ¶
,
b®´°¬
。
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}¤
,
~£
(
¤¥
),
jkl¼½CO2 uv
(犆i)z10:00£ºôÍ¿Á,»L14:00üÍ*Á。
ýuvÞwxmnig犆ilmB°tt,
Ûo¿vØVvÞwx£犆iÁõsv*z¤¥,Ç
L6vÞwx£犆iÁ<¾svµÎ,¿Þwx
jklig犆iÁY¤¥µo¸;V¿vÞw
x犆iÁ°ÁØÍ¿Á¤ÛK½7µo
¸
,
»6vÞwx°ÁisÃ
。
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vÞwxÜjklmnig²³§¨
(犜r)O«
ÔÛo´°±lm
,
Í*Á¤ÛL12:00
~14:00;ýuvÞwx¡¢£jklig
犜rY¤¥tÀ¹®£ºlm,ÜÝÞuvß
ΣºsvßÎ
,
tf*ÞwxÛÍoÃ
;
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{
,
t
½cd5sÃ
,
ÇL6vÞwxyz
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D ¤stuvÞwx
£jklRSó4Yi
«¬tu
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(
2),ig犘nY犌sØ犜rÔ®
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(犘<0.01),»Yi
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(犘>0.05);犆iY犘n®
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Ô®ÃÄtu
(犘<0.05),Yi
5®
tuÇsÃÄ
。
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§¨Üèßà»ß*åwzi¼í·
ßÎ
,
»Ù犆iº¿,Çswd
tṳ犆if¿È§¨f*´。
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,
jklmnigó4èvº
¿ûåNUig©u¼½CO2 uv
ÅÆ
,
»Y0usÎ
。
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/·ÅÆ
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i
Lÿbv
[17]。犉0(Ö×)、
犉m(ÍÎ)ýöⅡ(PSⅡ)Y.Vey
z?OJXØ?OuKÊ
,
»犉0 ¾
*YPSⅡY.VeD1 íçu[18]。V,
jklmnNUstbvÞwxÜ
,
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,
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,
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z½cdsÃÄ
,
ÇýÞwxyzY¤¥½ÔüU
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(犘<0.05),ÞwxÉPSⅡY
.Veûí¯N
(
/3,A);tK,ÜÞwxu
v¾*
,
jklmnig犉m Á®£ºlm,tf
¿vÞwx£ºsvÍÎ
,
ýÞuvyzY¤¥
½cdÔüUÃÄÊ
(犘<0.05),PSⅡY
.VeMÃjL¿ÞwxKiÃk
(
/3,B)。
Ú
,犉v(û)oFm YF0 cÁ,Q
ãÖMÃN
(QA)ãPG[19];»犉v/犉0、犉v/
犉m ÈÔûYPSⅡÍÎÃϨ。/3,C、DÃ
1
,
ÜÝÞwxuv¾*
,
jklmnig犉v/犉0
Ø犉v/犉m ÁÔç0kõ,ýuvyzY¤¥tÀ
Ô®ÃÄ£ºlm
(犘<0.05)。jklmn
igPSⅡY.VeiäÞwx»NU,
Lí·ØãÖZϨÃk
,
ä#$
ÚQó4ïð
。
YtK
,
ýuv NaClwx¤jkligPS
Ⅱ8Ó%Ϩ(ΦPSⅡ)、JXPSⅡY.VeÐ
LÚZϨ
(犉v′/犉m′)、ÑÒ
(狇P)ØY7UÙÑÒ
(犖犘犙)Ô@çÃÄ·ÅÆ(/4)。V,犉v′/犉m′å
x2 <=>LABij\?CDaUHM
Table2 Correlationmatrixbetweenmainphotosyntheticparametersandchlorophylindexesof犇犪狆犺狀犲犵犻狉犪犾犱犻犻leaves
Parameter 犘n 犌s 犆i 犜r Chla Chlb Chl(a+b) Chla/Chlb
犘n 1.000 0.963 -0.954 0.887 0.834 0.827 0.844 0.369
犌s 1.000 -0.723 0.820 0.829 0.836 0.826 0.722
犆i 1.000 -0.894 -0.725 -0.794 -0.747 -0.508
犜r 1.000 0.890 0.870 0.894 0.835
Chla 1.000 0.978 0.999 0.796
Chlb 1.000 0.986 0.668
Chl(a+b) 1.000 0.775
Chla/Chlb 1.000
n
: 1ÃÄtu(犘<0.05); 1
ÃÄtu(犘<0.01)
Note:indicatesasignificantdependence(犘<0.05);indicateaverysignificantdependence(犘<0.01)
stop1yz½cdüU0.05ÃÄÊ;£t
/3 Þwx£jklig犉0、犉m、犉v/犉0 Ø犉v/犉m
Differentlettersindicatesignificantdifferencesamongtreatmentsat0.05level.Thesameasbelow.
Fig.3 ImpactsofNaClstresson犉0,犉m,犉v/犉0and犉v/犉mof犇.犵犻狉犪犾犱犻犻leaves
6811 ! " # $ % & 36q
/4 Þwx£jklig犉v′、犉m′、狇P、犖犘犙 ØΦPSⅡ
Fig.4 ImpactsofNaClstresson犉v′,犉m′,狇P,犖犘犙andΦPSⅡof犇.犵犻狉犪犾犱犻犻leaves
PSⅡçÏ%Ã,YJX PSⅡY.
VeãÖÐZϨ
;狇På¤ãÖMÃN
e犙A Gdv,ûfYM
à MÃj§¨
;犖犘犙åPSⅡÇ07¡
s4zMÃj»f7¢:U£
Ñý
,
Y¤·¤U
。
L
¿Þuv
(50mmol·L-1)wx¡¢£,jklig
犉v′/犉m′À¤¥ç0£º,ΦPSⅡ、狇P、犖犘犙Y¤¥
tÀÂÃÄ·cd
;
L100~200mmol·L-1uv
Þwx¡¢£
,
ig犉v′/犉m′、狇PØΦPSⅡÔÜÝÞ
uvßλÃÄ£º
,
» 犖犘犙ÈÜ
ÃÄ<¾。
û{
,
LV*uvÞwx¡¢£
,
jklmnig
PSⅡ·¤¶·Ù%¥úf7¢:U
Àaßà
,
fkõwx¡¢£·¤¤
¦§
。
3 ¨ ´
Þwx¤#$QQ©ÅÆëzåáÙ
𪫬
,
L#øQNUwx»Ql
QzY.·bV
,
/·Øi
/·
toÃ
[20]。
°Â9
,
ÜÝÞwxu
v¾*
,
jklmn¦ó4§¨
(犘n)
kõ
,
ÇLævÞwxµeçãº
,
»6vÞ
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