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Morphology and Pollination Compatibility of the Pollen and Stigma in Robinia neo mexicana var. luxurians

多花刺槐花粉与柱头形态及授粉亲和性研究



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,2016,36(5):0938-0944
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犕狅狉狆犺狅犾狅犵狔犪狀犱犘狅犾犻狀犪狋犻狅狀犆狅犿狆犪狋犻犫犻犾犻狋狔狅犳狋犺犲犘狅犾犲狀犪狀犱犛狋犻犵犿犪犻狀
犚狅犫犻狀犻犪狀犲狅犿犲狓犻犮犪狀犪狏犪狉.犾狌狓狌狉犻犪狀狊
JIANGLina1,XUNShouhua2,ZHANGYuanshuai3,MAOXiuhong2,
QIAOYuling2,DONGYuanfu3,SUNBaiyou3
(1ShandongAgriculturalUniversity,Taian,Shandong271018,China;2ShandongAcademyofForestry,Jinan250014,Chi
na;3DaqingshanForestFarm,Linyi,Shandong273402,China)
犃犫狊狋狉犪犮狋:Weusedscanningelectronmicroscopy(SEM)inthisexperimenttoobservethepolenandstigma
morphologyof犚狅犫犻狀犻犪狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀狊indifferentperiodsofdevelopment,andhadtaken
photosforanalysis.Besides,wepolinatedthepolensof犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀tothestigmasof
another犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀andhighqualitycloneof犚.狆狊犲狌犱狅犪犮犪犮犻犪(犚.狆狊犲狌犱狅犪犮犪犮犻犪‘Luci
152’),andweusedanilinebluestaintoobservethegerminationprocessofpolentubes犻狀狏犻狏狅.And
then,weanalyzedthepolinationcompatibilityofthisspecies.Theresultsshowthat:(1)Theaverage
sizeofthepolenis32.08μm×19.55μm (poleaxislength×equatorialaxeslength).Theshapeofthe
polenislongspheroidicitywiththreegerminalfurrow,alittlesquare.Atthefloweringmoment,thepol
lengrowstothemaximum,itsmicroscopybecomesful.Thisisthebesttimetocolectthepolenof犚.
狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀狊.(2)Thesurfaceofthestigmahasmucilageandbulges.Ithasmuchmuci
lageat12hbeforefloweringandthereceptivityisthebest.At48hafterflowering,thestigmabeginsto
break,thereceptivityisalreadyverybad,andthewholereceptiveperiodofthestigmaisaboutthreedays
from24hbeforefloweringto24hafterflowering.(3)Thepolenof犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀could
germinateinboth犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀and犚.狆狊犲狌犱狅犪犮犪犮犻犪‘Luci152’treatedasthefemale
parent,buttheformergerminateslaterandelongatesslower,whichilustratesthatthepolenof犚.狀犲狅
犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀hasaffinitywithboththestigmasof犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀and犚.
狆狊犲狌犱狅犪犮犪犮犻犪‘Luci152’,buttheaffinitywiththeformerisworse.
犓犲狔狑狅狉犱狊:犚狅犫犻狀犻犪狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀狊;polen;stigma;SEM;polinationcompatibility
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e1 456758abc9Table1 Thesizeandshapeofpolensof犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀狊
ƒ­
Time
ŠÈ
Repetition
ÉÊË
(P)
Poleaxislength/μm
ÌÍÊË
(E)
Equatorialaxeslength/μm
P×E P/E ‰ÎShape
Õì24h
24hbeforeflowering
1 31.03~34.82(34.46) 14.06~22.23(20.55) 34.46×20.55 1.68 ËÏЉLongspheroidicity
2 31.33~35.01(34.28) 14.43~24.75(21.46) 34.28×21.46 1.60 ÏЉSpheroidicity
3 26.04~28.46(27.22) 10.69~20.84(18.61) 27.22×18.61 1.46 ÏЉSpheroidicity
4 31.83~32.87(32.43) 12.04~19.82(17.15) 32.43×17.15 1.89 ËÏЉLongspheroidicity
ÇÈAverage 32.10 19.44 32.10×19.44 1.65 ËÏЉLongspheroidicity
Õì12h
12hbeforeflowering
1 25.61~30.06(28.07) 15.64~22.07(18.68) 28.07×18.68 1.50 ÏЉSpheroidicity
2 31.93~34.84(32.95) 13.61~21.80(19.73) 32.95×19.73 1.67 ËÏЉLongspheroidicity
3 33.06~36.28(35.20) 12.05~23.42(19.21) 35.20×19.21 1.83 ËÏЉLongspheroidicity
4 30.65~35.41(34.98) 12.85~24.01(20.37) 34.98×20.37 1.72 ËÏЉLongspheroidicity
ÇÈAverage 32.80 19.50 32.80×19.50 1.68 ËÏЉLongspheroidicity
Õփ
Floweringmoment
1 32.43~36.55(35.04) 13.42~22.88(19.93) 35.04×19.93 1.76 ËÏЉLongspheroidicity
2 31.05~35.86(33.16) 11.02~23.08(20.25) 33.16×20.25 1.64 ËÏЉLongspheroidicity
3 34.45~38.28(36.17) 12.04~22.96(21.33) 36.17×21.33 1.70 ËÏЉLongspheroidicity
4 28.63~31.06(30.46) 12.26~21.42(18.91) 30.46×18.91 1.61 ËÏЉLongspheroidicity
ÇÈAverage 33.71 20.11 33.71×20.11 1.76 ËÏЉLongspheroidicity
Õï12h
12hafterflowering
1 29.44~34.06(32.44) 14.83~24.53(20.66) 32.44×20.66 1.57 ÏЉSpheroidicity
2 31.48~36.22(35.23) 14.45~25.61(21.21) 35.23×21.21 1.66 ËÏЉLongspheroidicity
3 29.54~32.28(31.25) 10.09~19.12(17.64) 31.25×17.64 1.77 ËÏЉLongspheroidicity
4 27.14~34.26(31.81) 17.77~24.26(21.50) 31.81×21.50 1.48 ÏЉSpheroidicity
ÇÈAverage 32.68 20.25 32.68×20.25 1.61 ËÏЉLongspheroidicity
Õï24h
24hafterflowering
1 22.48~30.76(29.56) 11.84~19.57(16.58) 29.56×16.58 1.78 ËÏЉLongspheroidicity
2 28.33~32.59(30.71) 10.67~23.03(20.03) 30.71×20.03 1.53 ÏЉSpheroidicity
3 27.17~30.73(29.48) 13.63~21.26(18.33) 29.48×18.33 1.61 ËÏЉLongspheroidicity
4 24.83~28.38(26.71) 12.41~21.89(18.80) 26.71×18.80 1.42 ÏЉSpheroidicity
ÇÈAverage 29.12 18.44 29.12×18.44 1.58 ÏЉSpheroidicity
4ÇÈGrandaverage 32.08 19.55 32.08×19.55 1.64 ËÏЉLongspheroidicity
049 ! " # $ % &                   365
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Fig.1 Theshapeofpolensof犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀狊
#2 ~12€‚_ƒ„†Åރßf
Fig.2 Thedetailofpolensof犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀狊indifferentperiodsofdevelopment
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Fig.3 Themorphologyofstigmasof犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀狊indifferentperiodsofdevelopment
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Table2 Thenumberandlengthofpolentubesgermination犻狀狏犻狏狅of犚.狀犲狅犿犲狓犻犮犪狀犪var.犾狌狓狌狉犻犪狀狊afterpolinatinginvivo
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Numberofpolentubes/stripe
2h 6h 10h 24h 48h
•†ï€ƒæ†ª‚Ëÿ
Lengthofpolentubes/mm
2h 6h 10h 24h 48h
~12×~12
犚.狀犲狅犿犲狓犻犮犪狀犪var.
犾狌狓狌狉犻犪狀狊×犚.
狀犲狅犿犲狓犻犮犪狀犪var.
犾狌狓狌狉犻犪狀狊
A1 10.67 10.33 13.33 15.67 11.67 1.00~2.00 4.00~6.00 7.00~11.00 12.00~15.00 15.00~19.00
A2 10.00 14.50 11.00 16.50 12.50 1.00~3.00 4.00~6.00 7.00~11.00 11.00~15.00 14.00~18.00
A3 16.33 9.00 10.33 10.67 17.67 1.00~3.00 4.00~7.00 8.00~12.00 12.00~16.00 15.00~19.00
ÇÈ
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