免费文献传递   相关文献

Effects of Different pH on Growth and Ergot Alkaloids Concentrations of Symbiont of Epichlo gansuensis Achnatherum inebrians Seedling

不同pH对醉马草内生真菌共生体幼苗生长和产碱的影响



全 文 :书!"#:10004025(2016)04071506               犱狅犻:10.7606/j.issn.10004025.2016.04.0715
  %&(:20151125;)*&%+(:20160309
  ,-./:(“973”)*(2014CB138702);(+,-%./(31372366);0123456789:(IRT13019)
  0123:;<=(1992-),>,?@ABCDE,CDFGHIJKELMNEO。Email:wanzhw14@lzu.edu.cn
  PQRS:TUV,WB,0X,CDFGHIJKELMNEOYJZ#[\%。Email:chunjie@lzu.edu.cn 45狆犎6789:;<=>;? @A;BCDEFGH !"# , %&

()

* +,,-.

]^_% J`ab-c%d

J`abEefg(hijkl

]^730020)
I J:mCDnopqrpH(3、5、7、9s11)t\uvwJKELMNEOxyz{e|}、~€、‚ƒ„
…Y†‡ˆ‰s†‡4Š‹ŒzŽ

…NEOxyE‘s’Šz“”pH•–。—˜™:(1)vwJKE
LMNEOxyš›

œ‘

žsEŒŸ ?pH7¡(¢£“_)¤¥›¦§¨4©ªŠt\,«¬4©E‘ |}?­Šƒt\® ¤¥›¦­ªt。(2)xy~€‹Œ¯?pH7z°ƒ•–®(¢£“›)¤¥›¦ pH3、pH5spH11t\,¬­Šƒt\®~€‹Œ¯¤¥›¦­ªt\,±xy‚ƒ„‹Œ?qrpHt \²³´µ¤¥¶· 。(3)?¸r¡³K,vwJKELMNEOxy†‡ˆ‰s†‡4Š‹Œ ¹º»pH¼½ ›«¾¿ ; ?t\¡³H15d¡,†‡ˆ‰?pH9~11t\®ÀÁŒ“Â,†‡4Š‹Œ?pH11t\®¢£“ _¼ , ªƒ•–ÃÄvwJKELMNEOxy’Š 。 CD7Å , vwJKELMNEOxy?°ƒº»•–® E‘“Æ , ¬?Šƒ•–®E‘Ǫƒ•–®E‘ÈÉ , «OK†‡ˆ‰s†‡4ŠzÀÁ?­Šƒ•–®¢£ ʼ , ËvwJKELMNEOÌͦΊ`zÏз 。 KLM : vwJ ; KELM ; †‡ˆ‰ ; †‡4Š ;pH NOPQ
:Q946.88;S452   !RSTU:A
犈犳犳犲犮狋狊狅犳犇犻犳犳犲狉犲狀狋狆犎狅狀犌狉狅狑狋犺犪狀犱犈狉犵狅狋犃犾犽犪犾狅犻犱狊犆狅狀犮犲狀狋狉犪狋犻狅狀狊狅犳
犛狔犿犫犻狅狀狋狅犳犈狆犻犮犺犾狅犵犪狀狊狌犲狀狊犻狊犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊犛犲犲犱犾犻狀犵
WANZhiwen,FENGJiangrong,CHENZhenjiang,CAOYing,LIChunjie
(KeyLaboratoryofGrasslandFarmingSystems,ColegeofPastoralAgricultureScienceandTechnology,LanzhouUniversity,
Lanzhou730020,China)
犃犫狊狋狉犪犮狋:AstudywasconductedtoinvestigatetheeffectsofdifferentpH(3,5,7,9,11)onvariationsof
growthandergotalkaloidsconcentrationsinsymbiontof犈狆犻犮犺犾狅犵犪狀狊狌犲狀狊犻狊犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seed
ling.Theresultsshowedthat:1)theplantheight,rootlengthandbiomassof犃.犻狀犲犫狉犻犪狀狊hadpeakvalue
underpH7,andhadsignificantdifferences(犘<0.05)thantheotherfourpHtreatments,andthefour
kindsofgrowthindexunderstrongalkalinetreatmentweresignificantly(犘<0.05)higherthanthatunder
acidtreatment.2)ChlorophylcontenthadmaximumunderpH7,whichhadsignificant(犘<0.05)differ
enceshigherthanthatofpH=3,5andpH11,andunderstrongalkalinetreatmentweresignificantly(犘<
0.05)morethanacidtreatment.SolublesugarunderfivepHtreatmentsdifferencewasnotsignificant
(犘>0.05).3)ThecontentsofergineunderpH9-11hadapeakvalueat15d,whichhadsignificant(犘<
0.05)differencewithothertreatments.ThecontentsofergonovineunderpH11hadmaximumat15d,
whichweresignificantly(犘<0.05)enhancedbyincreasingpH.Tosumup,Symbioticseedlingsgrowbest
underneutralconditionsandunderalkalineconditionsgrowwelmorethanthatunderacidicconditions.However,
theaccumulationofergineandergonovinehadpeakvaluesunderstrongalkalineconditions.Comprehensivegrowth
andergotalklioldproduction,thesymbiontscanbeusedinthecultivationofsalinealkalilandgreening.
犓犲狔狑狅狉犱狊:犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊;endophyte;ergine;ergonovine;pH
  vwJ(犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊)ÑIÒ-ÓÓ

(犃犮犺狀犪狋犺犲狉狌犿)ÂÕEJÒ#,?KÖ×、4 Ø 、 !Ù 、 ÚÛ 、 ÜÝŸÞ ( ß )  µà , Ñ°"F á,JâãäzåƒæJ²ç [1]。 IJKELMÑ |E‘?#šOKèéêë2ì_2Eíîï , « qðñã#¤òó2ôõzçZLM
[2],
ö÷µ
¾­øãùúƒ

û›üýþÿƒŸ!i

vwJ
"犈狆犻犮犺犾狅ÔKELM#R’E†‡4Šs†
‡ˆ‰

2%EŠ&()Ìͦ*+,,&-
.j…ð/0î123Œ45
[3],
†‡4Š6
7Rͦ89:

u;>’<_=2÷µhä>
?
[4],
@A÷µhäzBÍC7D¼

EçFF

v
wJKELMNEOG’Ez†‡ˆ‰s†‡4Š
u(H
[5]
sIJJK÷µçLzæƒ
[6],
Ë§R
HEaBC7MÍ 。 2%EŠz‹Œ"E
@8sNE@8 [7] µOPzQf 。 ¹RSbzTU78 , VWª·?°XF` ßYH¤ , ãäà?‘Z…Xz[、 ][\` ßY!X^ 、 _W` [8], §°Sbab 、 ·csaB z_ŒðÍÑ¿TVWª·zãäâ@ 。 VWª· deVWµæ/Ôf8íg¾¿ 、 ch45sVW —i¶j , 荎VWkEzíl

mno€zp
·"qr

µstzuê"v

kŒo€zµ?ƒ
…YVWwxnzyhŸ
[9]。
?°z"{â

|"{â

!"K}`ßY~Û`ß

VWΊ·ê
H€Eeº»sabx78zhä@€
[10]。
pHu#E‘71YHhä,ö‚…67Ž #
,¯
³7PƒŽVW、 ·%sE
%„ƒ…熍Ž#E‘71 [11], ã䍎#
{eó‡

tˆ‰s’Œ 。 Š&‹ , “”u\Mv wJ%8Œ7zªŠŽÑpH6~9,KELM? ª€ (pH4~5)®…¤¥û›%87‘,? ªƒ’°ƒ (pH4~7)•–®…û›xy“h, ?°ƒ’­Š (pH7~11)€®…û›%8z” ‘Y”œ‘ ; ?ú»º»® , •“–€s΀  y¾¿vwJKELMNEO†‡4Šs†‡ˆ ‰z‹Œ [12], ±ÑQ¦ªŠ€®vwJKELM NEO†‡EŠzÀÁzCD*—˜™&‹

Қ
k›?qrpHt\®novwJKELMNEO
xyzE‘s’Šœ

Pƒ›?ž¸Ÿ ¡¢§†
‡4Šs†‡ˆ‰z‹Œ¶·

…vwJKEL
MNEOxyE‘sEŠÀÁz“”pH•–。 1 £¤sF¥ 1.1 VWXY škGÍzvwJ%8¦2014Õ9¦§¨+ ÜÝÝX (100°33.139′E,37°13.823′N),2015Õ4 ¦Pƒ©¡ , ¹sª«1šKELM犈狆犻犮犺犾狅 犵犪狀狊狌犲狀狊犻狊¬­zvwJ`z%8ͦҚk。 1.2 XYZ[\]^ ®ª™F¯° , ±²³´zvwJ%8µ%¦ ¶µ300g·¸z¹º»¼½(0¾15cm,¿¾10 cm,À12cm)°,?]^_%Á°ÂßÃyÄl[Å îï12hÅÆ,Äg(20±1)℃,ÅÆ­g120 μmol·m -2·s-1]KÐnxy。ÇîLŒÈÉ200 mLHoaglandmnž。ÊvwJxyE‘£5î Ë< , …1mol·L-1NaOH ‚žs1mol·L-1 H2SO4 ‚žÌÄpHÍH3、5、7、9s11zªŠ žt, «100mLt\xy,ÍÎÏPHS3CÐO pH9(pH¢ŒŽ2~14,` ÛÐO-%ÑÒµÓ ÔÕ ) ¡k‚žpH¼。Ç©t\5Öh×,ÇØ3 d…ÙçÖt\,?t\<3、6、9、12s15dÍ« vwJKELMNEOxy¡¢§†‡ˆ‰s†‡ 4Š‹Œ 。 t\15d<,Í¢LvwJKELM NEOxy?qrpH t\®zš›、œ‘、ž Ú 、 EŒ

~€s‚ƒ„

1.3 _`aSbcd
1.3.1 efaS_` ۚvwJKELMNE
Oxyzš›sœ‘¨Í¢Œ¥

ۚxyžÚ
¨Í9Ú¥

ۚxyEŒz¢L¨Í܌¥ 。 1.3.2 ghijk vwJKELMNEOxy ~€z¢L¨ÍÝÄÞûߟ¥ 。 Ë4àvwJ xy~áâê2mmãäzåá,Ü«0.1gæç ¿è10mLû«ž(ºé∶êë=1∶3)?lÄì 핖®Þî24h,ï³ïðÚÖ。«`ñž? 652nmò‘t¢LóżA652,èô•®Ôõ¢L ~€z‹Œ (mg·g-1):犆=犃652×10/(34.5× 犿),犿HÜæŒ[13]。 1.3.3 lmnojk ‚ƒ„‹Œz¢L¨Í öëߟ¥ 。 Ü«öë200mg¦100mL÷øª °ù‚v , ÅÌêöëšú 。 Ü«~áàæ0.1g rèšû° , ¿üýb5mL,þ0¦ÿb°û«1 h,û«žƒ!’25mL"Œ#,Íüýb×%&
(èL"’)g

ó«Ê¢ž0.2mL,¿èšû
617 ! " #  % & 36* °, +¿üýb2.3mL,ï,,¹<-šû./U¿ èöëšú6.5mL,01ï,,2šû3`45’ lÄ , …6¿öëšúsüýbz78ûRuÆ , ? 620nmò‘t,ôÂiÂ9¥¢LÊ¢û°û«ž ‚ƒ„‹Œ [14]。 }9:; :狔=0.111狓+0.0138, 犚2=0.988。 1.3.4 pqrsCpqtEjk ËÊ¢vwJ xy?-20℃z<=<>5h,è?4>“?s° -60℃“?24h<,«=U¦C@°CAêBC。 Ü«50mgæ綦1.5mLzEppendorffDû ° ; ¿è1mLû«ž(CHCl3∶MeOH∶NH4OH =75∶25∶2),EFG,,lÄì핖®ƒH。< -15℃、10000r·min-1•–®fD5min,¦P IJ°ì핖®+,K7“? 。 Ç©fDû¿è 0.75mLMeOH∶CCl4(1∶2)s0.25mL25mmol ·L-1L¸ª,EFG,<,¦fDs(Beckman, Germany)°?21℃、12000r·min-1•–®fD 5min,ó«`ñž0.25mL-0.22μmM¾zµ s¸ƒ!Nƒ!’1.5mLOŸŸ #,ÊÍ[15]。 Í Agilent1100series›?ž¸Ÿ fg (Agilent,USA),EclipseXDBC18¸(250mm×
4.6mm,5μm)Ÿ P,3l¸HA(0.1mol·L
-1
NH4OAC)sB(ºQ),ôÆ0~5minAžz÷g
R95%~85%、5~10minA žz÷gR85%~
80%、10~15minAžz÷gR80%~90%、15~
20minAžz÷gR90%~95%zSg&T;¡¢
ò‘
(Ex)312nm,7Uò‘(Em)427nm[11],3U
1.0mL·min-1,…æŒ20μL,ˆ‡ˆ‰s†‡
4Š}æÍVq’0.375、0.75、1.5s3mg·
kg-1÷g,W«¸r÷gSgzX%}æGu,…
ó}¥Y0¸Q}96;FZ

PƒŸ ÚŠSR
[\–
(ChemStationforLCRev.A.10.01,USA)
]¢…Zè¢LʼFÀ

œŠ¸QFZsæçV
qz^Ú

9_æç°X%EŠz÷g 。 1.4 uv]^ ¨ÍSPSS22.0g9`\–…Ùja¤¥ƒ ` , ÍDuncan¥…ÙÂhßÇ。 2 —˜"` 2.1 狆犎6789:;<=>;?@A;BFGH b™1c,¹RpHz½›,vwJKELM NEOxyš› 、 œ‘ 、 žsEŒ de¾¿<
®4zfg

è ?°ƒ•–
(pH7)¡ ¢£“_
¼

¬¤¥›¦§¨4©ªŠt\(犘<0.05)。x
yš›sœ‘?pH5spH9t\®™¤¥ja
«öh ¤¥›¦pH3spH11t\;pH11t
\zš›i¤¥›¦pH3t\,±Xt\³œ‘
™¤¥ja
(犘>0.05);xyžÚsEŒ?pH 5t\® ¤¥›¦pH3、9、11t\,«pH9、11 t\i_¤¥›¦pH3t\。j,vwJKE LMNEOxy?°ƒº»®E‘“Æ , kªkŠ º»® (pH5spH9)Ö²,?¾­zªŠº»® (pH3、pH11)lj。 2.2 狆犎6789:;<=>;?@AghiC lmnojkFGH   b™2c,¹Rt\pHz½›,vwJKE LMNEOxy~€‹Œde¾¿<®4zf g , è?pH7¡¢£“_¼,¬¤¥›¦pH3、5 s11t\;xy~€‹Œ?pH5、9t\®i¤ ¥›¦pH3、11t\,«pH11t\i¤¥›¦ pH3t\。vwJKELMNEOxy‚ƒ„ ‹Œ?qrpH t\²³´µ¤¥¶·。j,v wJKELMNEOxy~€‹Œuº»pH
w1 45狆犎]^x789@Ayz、{B、P|C
;}kF~
Table1 Theplantheight,rootlength,tileramountand
biomassof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seedlingsunder
differentpHtreatment
pH
š›
Plantheight
/cm
œ‘
Rootlength
/cm
ۚžÚ
Tileramount
perplant
ۚEŒ Biomass perplant/g 3 32.87±0.39d 19.19±0.11c 3.05±0.05d 0.16±0.01d 5 36.59±0.18b 21.90±0.22b 3.70±0.12b 0.27±0.01b 7 38.65±0.81a 23.12±0.33a 4.20±0.12a 0.30±0.01a 9 37.15±0.10b 21.46±0.16b 3.30±0.12cd 0.21±0.01c 11 35.14±0.57c 20.02±0.71c 3.40±0.10bc 0.20±0.01c   m:™°ÚŠH{ ¼±}9n(n=5);roqrpq™òt\³? 0.05b{r?¤¥ƒja(犘<0.05);®r。 Note:Dataarepresentasmean±SE(n=5);Thedifferentnormalletters withinthesamecolumnmeansignificantdifferenceatthe0.05level;Thesame asbelow. w2 45狆犎]^x789@AghiC lmnojkF~ Table2 Thecontentsofchlorophylandsolublesugarin 犃.犻狀犲犫狉犻犪狀狊seedlingsunderdifferentpHtreatment pH ~€ Chlorophyl /(mg·g-1) ‚ƒ„ Solublesugar /(mg·g-1) 3 2.12±0.22d 0.02±0.001a 5 3.25±0.09b 0.02±0.001a 7 3.82±0.06a 0.02±0.001a 9 3.39±0.05ab 0.03±0.001a 11 2.71±0.28c 0.02±0.001a 7174ï ;<=,Ÿ:qrpHuvwJKELMNEOxyE‘s’ŠzŽ w3 45狆犎]^x789@ApqrsCpqtEjkF~ Table3 Theergineandergnovinecontentsin犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seedlingsunderdifferentpHtreatment †‡E$Š Ergotalkaloids pH t\¡³ Treatmenttime/d 3 6 9 12 15 †‡ˆ‰‹Œ Erginecontent /(mg·kg-1) 3 14.83±0.27cD 19.84±0.44cB 28.84±0.84cA 16.85±1.01dC 7.76±0.19dE 5 15.50±0.27cD 23.83±.050bBC 36.21±0.38bA 26.09±0.91cB 23.16±1.62cC 7 16.09±0.51bcE 24.14±0.36bD 38.30±0.58abC 54.22±1.75bB 62.84±2.40bA 9 17.14±0.57bD 26.05±0.77bC 38.60±0.75abB 61.53±2.78aA 68.54±5.81abA 11 19.89±0.53aE 29.69±1.33aD 40.64±1.17aC 64.57±2.93aB 73.46±2.03aA †‡4Š‹Œ Ergnovinecontent /(mg·kg-1) 3 16.69±0.41cC 21.58±0.51dC 67.32±3.00cB 79.31±3.93dA 70.97±2.40dB 5 18.45±0.47cC 26.39±1.51cdC 89.72±4.04bA 93.63±3.36cA 71.49±2.60dB 7 20.86±0.65bE 32.50±0.54cD 96.11±3.72bC 113.11±4.63bB 172.96±3.67cA 9 22.59±0.68bD 44.85±3.13bC 122.14±2.19aB 129.37±3.46aB 205.40±3.26bA 11 29.67±0.84aD 57.66±3.68aC 129.92±4.07aB 140.09±3.35aB 233.29±8.66aA   m:™°ÚŠH{ ¼±}9n(n=5),ro°zqrstpq™òrït\³ja¤¥(犘<0.05),«rÙqr_tpq™òt\¡³ ²³ja¤¥ (犘<0.05)。 Note:Dataarepresentasmean±SE(n=5);Thedifferentnormalletterswithinthesamecolumnmeansignificantdifferenceamongtreat mentsatthe0.05level,whiledifferentcapitalletterswithinthesamerowmeansignificantdifferenceamongstagesatthe0.05level. Ìuv , è"§E‘$̄w¸xu 。 2.3 狆犎6789:;<=>;?@Apq;} EjkFGH 2.3.1 pqrsjk b™3c,¹Rt\¡³ zy‘ , vwJKELMNEOxy†‡ˆ‰‹Œ ?pH3、5ªƒt\•–®de¾¿<45zf g , è ?t\9d¡¢£“_¼,¬¤¥›¦§z t\¡³ , A¡Íßt\3d¡¤¥½›94.5% s133.6%;«?pH7、9s11t\•–®,xy† ‡ˆ‰‹Œd{|`½zfg ,  ?15d¢£“_ ¼ ,¯ _¤¥›¦§zt\¡³ , A¡Íßt3d¡¤¥½›290.6%、299.9%s269.3%。r¡, ?¸rt\¡³K , xy†‡ˆ‰‹Œ¹Rt\ž pHz½›«¾¿;¦t\15d¡,ªƒt\(pH3、 5)¤¥5¦§¨°ƒ、Šƒt\(pH7、9s11),« pH11t\i¤¥›¦pH7t\(犘<0.05)。 2.3.2 pqtEjk b™3}c,¹Rt\¡ ³z¾¿ , vwJKELMNEOxy†‡4Š‹ Œ?pH3、5ªƒt\®¯de¾¿<45zf g ,  ?t\12d¢£“_¼,A¡ÍÑt\3d ¡z4.75^s5.07^;«?pH7、9s11t® , †‡4Š‹Œ¹Rt\¡³z¾¿d{|`½ zfg , è ?t\15d¢£“_¼,A¡ÍÑ t\3d¡z8.29、9.09s7.86^。r¡,?¸r t\¡³K , xy†‡4Š‹Œ¹Rt\žpH z ½›«¤¾¿ , ¬t\³zja¹t\¡³y‘ /U¿_ ; ?t\15d¡,ªƒt\(pH3、5)¯ ¤ ¥5¦§z°ƒ 、 Šƒt(pH7、9s11),¬° ƒ 、 Šƒt\³¯r?¤¥ƒja (犘<0.05)。 …`—˜~ , ‘¡³zªt\ÃƇˆ‰ s†‡4ŠzÀÁ , «Ç‘¡³zŠƒ•–t\€ …NEOxyOK†‡ˆ‰s†‡4Šzuê 。 3  ‚ 3.1 狆犎\789:;<=>;?@A;BFK€ pHэŽ#$E‘sÀÁÖEˆ‰$tzh 亻@8 [16]。 qr#$E‘Gƒ“”pH Ž µGjÍ , •s†E‘z“”pH H6~7,„¼? pH6~8ŽKE‘“Æ,pH5~6u…JzE‘ “µM 。 µ†#$uªŠgä‡ßLj‰ , •Šs ‹Œ¼yE‘¦ªƒVW [17], «Žs¼‘’ @“”«•–E‘?°ƒ’kŠƒVW [18], «µ† #$z”̍ŽÇ( , ߕw—˜?pH4~8Ž K ™ÝE‘ , ?pHH5.6~6.0ŽKE‘“ É [19]。 çFF ,pH‚…67Rͦ#$,•ƒ› zpH?çLZg`ÃÄ#$œšzE‘,…« Ž§ubsnzó› ,¯ ŽœKz ATPzuêUg,…«de#$OKz$tyŒž ÌqŸ [20]。 •?­ªƒ (pH<5.5)¬Al³sg› zVW° 45#$uCazó›è¡¢œKCa z™Ý£¤sÄ , …«Ž" CaµQzE\… Z [21]。 EçFF ,pH ¯PƒŽWZmno€ zµ?ƒ¥³7Ž#$zE‘71 , ¦•?pH6 ~7ŽK§zµ?ƒ“_[22],¨ 、©sªŸkŒ o€?­ª•–®‚vƒ­ ,  u#$’Eæ« 。 š› 、 œ‘ 、 žsE$ŒÑ¬Œ#$E‘71 zhä{e|} , ÒCD™ , ƒ5샛zpH  qM¦vwJKELMNEOxyzE‘ , ?pH H7z°ƒ•–®NEOxyzš›、œ‘、žs E$Œ ¢£“_ 。 ­O¥® , vwJKELMN 817 ! " # $ % & 36* EOxy…`4©{e|}?pH11­Št\®  ¤›¦pH3­ªt\,~¸u¦­ª€ «¯§u­Š€÷µl­z°±ƒ 。 ÅuRÍÑ Ÿ#$“.ÒzE\Ee„w ,¯ Ñ#$E‘G ƒ$tsyŒzh䥲 。 ~€‹Œz³67 Ž#$ÅuRÍz­k , #$±£ú»€¡ , W %E\ƒZ,±£Ž , R«67ì³7Ž£ #$~€z‹Œ [23]。 ‚ƒ„H´µ£¤$t , Ñ#$ùúRÍ°ç©häz´µ£¤@8 ,¯ Ñ #$O°häzy²s¶² 。 µ%SCD7Å , 5 pHy45·~€‹Œ,›pHu~€‹Œ™ ¤¥Ž ,pH6t\®¸¹‚ƒ„‹Œ“›,pH ƒ›ìƒ5u~၂ƒ„‹Œ,µ¤¥45R Í [24]; /º¼~€‹Œ?Šƒ•–®Çªƒ•– ®› , ¬E‘lÆ [23]。 ÒCD™ , ?pH7z°ƒ •–® , vwJKELMNEOxy~€‹Œ¢ £“_ , «?­Šƒ•–®~€z‹Œ¤›¦ ­ªƒt, $Ñ@H#$œ°r?Rqrz pHß,§°~O.tzpH »?8.0ãä, ڼµ?“”zpH ®½µ“›zE$íƒ,… w.#$™ÝzE‘71 。 ÒCD°vwJKEL MNEOxy‚ƒ„‹Œ?pH9kŠƒ•–® ¾›¦§¨t, ±?W©t\³jaq¤ 。 3.2 狆犎\789:;<=>;?@Apq;} EjkFK€   †‡ŠHÛ¿ÀÁZEŠ[7],ц‡ªzÂ
E , ÑçZ…¾¿ñã#u``
、`
®IJl
ùƒzLMæ€ [25]。 †‡ˆ‰s†‡4ŠÑv wJKELMNEO’Ez2%häz†‡Š[26], X%EŠzr?…ðNEO#šÃ±JKz ¨I [27], u§{êwÄRÍ 。 Ž†‡4Šs†‡ ˆ‰‹Œz@€µE@8sNE@8 , E@
8•vwJz.@Å

KELMz.@ÅsÆÄX
Suêz¼Ÿ

NE@8µÄg 、 ÅÆ 、 b 、 VW •–sWZmntzžÇŸ
[2829]。
TUV
[15]
C
D7Å

¹RvwJE1ïzy‘

†‡ˆ‰s†‡
4Š‹Œde¾¿<®4zfg

¬qrz#šÒ
È?qrzE1ïG‹2%†‡ŠŒµGja,?
Éïxy~Ê°EŠ‹Œ›¦~á , «ËËxy sêš~á°EŠz‹Œ›¦~Ê

ÌÍÎ
[30]
CD™

ÏЛgz¾¿µM¦†‡4Šs†‡
ˆ‰zÀÁ

«Ç›zÏÐё»𧋌=Å
Ò¾‘

Ó¿çLŒzó²ŸÔª

Õcs§c 
û›X%†‡ŠzÀÁ [31], ¬“–€s΀ µM¦¾¿vwJKELMNEO†‡ˆ‰s† ‡4Šz‹Œ [12,32]。 îÖן [33] CD™ , 5Ā ð°zØÙKELMNEO†‡ŠzÀÁ¤¥ ¾¿ , ~ú»•–uNEO†‡Šz’E÷µç Lz€…RÍ 。 ҚkÚÖnopvwJKELM NEO?qrpH t\®z’Šœ,CD—˜™  , Šƒ•–µM¦†‡ˆ‰s†‡4ŠzÀÁ , « ªƒ•–ÃÄvwJKELMNEOxy’Š 。
çFFyÑ@HX%†‡ŠHÛÅzÔ.†‡ Š , l”Ü?Šƒ•–®uê , EçFFyÝuê ƒZ°z¼µÞ_zQf 。 ?†‡EŠzuêƒ
Z°ß"Þ¼

§°àáVê.ŸÔªu¼
(dimethylalytrytophansynthase,DMATS)&.
Ñ⩆‡EŠzuêƒZ°zÓU¼ [34], Š ƒ•–yð§íƒl›«µM¦†‡4Šs†‡ ˆ‰zÀÁ , ±pH uvwJKELMNEOxy ’Šz÷ORÍs\}µÊ…ç†CD ҚkuvwJKELMNEOxyzE‘s †‡EŠÀÁz“”pH •–…ÙpCD,7Å
?pH7z°ƒ•–®xyE‘“Æ,¬xyùŠ
ƒ­¦ùªƒ

«xy†‡ˆ‰s†‡4ŠÀÁz
“”•–HpH9~11z­Šƒº»。ãunäx
yE‘s’ŠŒœ

ËvwJKELMNEO
?Ί`ß_FÀ%#…wxbVs·º»

‚!R

[1] å<æ.°J`häµæ#[M]."ç:°ab=èé, 1997:166176. [2] SIEGELM R,LATCHGCM,JOHNSO MC.Fungalendo phytesofgrasses[J].犃狀狀狌犪犾犚犲狏犻犲狑狅犳 犘犺狔狋狅狆犪狋犺狅犾狅犵狔, 1987,25:293315. [3] YOUNGCA,SCHARDLCL,PANACCIONEDG,犲狋犪犾.Ge netics,genomicsandevolutionofergotalkaloiddiversity[J]. 犜狅狓犻狀狊,2015,7(4):12731302. [4] MCCOLLOUGHSF,PIPERE,LOHNSONZB,犲狋犪犾.Effect ofthefescuealkaloidsonperipheralbloodflow,serumprolac tin,andrectalterperaturesinsteers[J].犑狅狌狉狀犪犾狅犳犃狀犻犿犪犾 犛犮犻犲狀犮犲,1994,72(Suppl.2):31(Abstract). [5] SCHARDLCL,PANACCIONEDG,TUDZYNSKIP.Ergot alkaloidsbiologyandmolecularbiology[J].犜犺犲犃犾犽犪犾狅犻犱狊: 犆犺犲犿犻狊狋狉狔犪狀犱犅犻狅犾狅犵狔,2006,63:4586. [6] ZHANGXX,LICHJ,NANZHB,犲狋犪犾.犖犲狅狋狔狆犺狅犱犻狌犿en dophyteincreases 犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊 (drunken horse grass)resistancetoherbivoresandseedpredators[J].犠犲犲犱 犚犲狊犲犪狉犮犺,2012,52(1):7078. [7] TITTLEMIERSA,DRULD,ROSCOEM,犲狋犪犾.Occurrence ofergotandergotalkaloidsinwesternCanadianwheatand othercereals[J].犑狅狌狉狀犪犾狅犳犃犵狉犻犮狌犾狋狌狉犪犾犪狀犱犉狅狅犱犆犺犲犿犻狊 狋狉狔,2015,63(29):66446650. [8] ¦áç,êëì,¸ Zí,Ÿ.VWª·î«YïðcñCD… 8 [J].Ee%ò<,2014,33(11):31373143. YUTY,SUNXS,SHICR,犲狋犪犾.Advancesinsoilacidifica 9174ï ;<=,Ÿ:qrpHuvwJKELMNEOxyE‘s’ŠzŽ tionhazardsandcontroltechniques[J].犆犺犻狀犲狊犲犑狅狌狉狀犪犾狅犳犈 犮狅犾狅犵狔,2014,33(11):31373143. [9] óôõ,ö{{,÷ ø,Ÿ.VWª·uùú’ŒzŽYç %°ªƒßÇ [J].RCD,2014,28(6):585588.
YUANZG,CHENPP,TANGK,犲狋犪犾.Effectsofsoilacidifi
cationonyieldoflatericeanddifferencesinacidresistancea
mongvarieties[J].犆狉狅狆犚犲狊犲犪狉犮犺,2014,28(6):585588.
[10] Ìûü,ýûþ,ÿuÍ,Ÿ.GuΊu¼‘’yïœf
„wzŽ
[J].°Eeab%&,2013,21(3):340346.
ZHANGXL,LIUXJ,QIMX,犲狋犪犾.Impactofsaltandalka
listressongrowthofalfalfainseedingstage[J].犆犺犻狀犲狊犲
犑狅狌狉狀犪犾狅犳犈犮狅犃犵狉犻犮狌犾狋狌狉犲,2013,21(3):340346.
[11] !"#.VWªŠƒ"#zE‘[J].KÖ×ab-c, 2003,6(33):4142. ZHAOJX.StudiesonthesoilpHandplantgrowth[J].犐狀狀犲狉 犕狅狀犵狅犾犻犪犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲犪狀犱 犜犲犮犺狀狅犾狅犵狔,2003,6 (33):4142. [12] ZHANGXX,LICJ,NANZB.Effectsofsaltanddrought stressonalkaloidproductioninendophyteinfecteddrunken horsegrass(犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊)[J].犅犻狅犮犺犲犿犻犮犪犾犛狔狊 狋犲犿犪狋犻犮狊犪狀犱犈犮狅犾狅犵狔,2011,39(4):471476. [13] ARNONDI.Copperenzymesinisolatedchloroplasts.Poly phenoloxidasein犅犲狋犪狏狌犾犵犪狉犻狊[J].犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔,1949, 24(1):115. [14] TuE.#E\E·jkâ\"cñ[M]."ç:›Ÿ01
=èé
,2000:134278.
[15] TUV.vwJKELMNEOE%"Ee%„ƒzCD [D].]^:]^_%,2005. [16] ÷ ø,%=,î=4,Ÿ.VWpHu#E‘71Žz CD…8 [J].RCD,2013,27(2):207212.
TANGK,ZHU W W,ZHOU W X,犲狋犪犾.Researchprogress
oneffectsofsoilpHonplantgrowthanddevelopment[J].
犆狉狅狆犚犲狊犲犪狉犮犺,2013,27(2):207212.
[17] ö&,(û),!%­,Ÿ.*Ò#”̪ƒVWs\z CD…8 [J].VW,2015,47(2):252258. CHENRF,DONGXY,ZHAOXQ,犲狋犪犾.Advancesina daptivemechanismstoacidsoilsinwoodyplantswithanem phasisonlespedeza(犔犲狊狆犲犱犲狕犪犫犻犮狅犾狅狉)andoiltea(犆犪犿犲犾 犾犻犪狅犾犲犻犳犲狉犪)[J].犛狅犻犾,2015,47(2):252258. [18] + ,,w-.,T /.¼‘’mnêYãäE
ƒ
[J].Jb-%,2003,20(10):3940.
WANGX,MAYX,LIJ.Alfalfanutrientcompositionand
mainbiologicalcharacteristics[J].犘狉犪狋犪犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲,
2003,20(10):3940.
[19] ö-o,ö01..@ÅspH uw—˜TæyE‘zŽ
[J].%8,2015,34(7):2025.
CHENK Y,CHEN Y Y.EfectsofgenotypeandpHonthe
growthofvirusfreepotatoplantlets[J].犛犲犲犱,2015,34(7):2025.
[20] !02,Ì=3,Ÿ.›pHu#E‘71zŽY§8E %CD…8
[J].°Eeab%&,2008,16(3):783787.
ZHAOYK,ZHANGWS,犲狋犪犾.Researchprogressinphysiology
andmolecularbiologyofplantresponsestohighpH[J].犆犺犻狀犲狊犲
犑狅狌狉狀犪犾狅犳犈犮狅犃犵狉犻犮狌犾狋狌狉犲,2008,16(3):783787.
[21] 456,74z,89U,Ÿ.”u#ùúyhzŽY§ Ee%:; [J].ÌÍEe%&,2005,16(5):971976. JIANGTH,ZHANXH,XUYCH,犲狋犪犾.Rolesofcalcium instresstoleranceofplantsanditsecologicalsignificance[J]. 犆犺犻狀犲狊犲犑狅狌狉狀犪犾狅犳犃狆狆犾犻犲犱犈犮狅犾狅犵狔,2005,16(5):971976. [22] Ì<,= ò,Ì×/.a>VW§€Ni²?­CD…8 [J].1Xab_%%&,2004,19(4):453456. ZHANGNM,HONGB,ZHANGYJ.Studyprogressofphos phorusnonpointsourcepolutioninfarmland[J].犑狅狌狉狀犪犾狅犳 犢狌狀狀犪狀犃犵狉犻犮狌犾狋狌狉犪犾犝狀犻狏犲狉狊犻狋狔,2004,19(4):453456. [23] @-A,BC-,DE,Ÿ.mnžpH u3%FÒ#E‘ s~€GÅzŽ [J].!"#%&,2009,29(2):338343.
WUYY,XINGDK,犲狋犪犾.EffectsofpHongrowthcharac
tersandchlorophylfluorescenceofthreevineplants[J].犃犮狋犪
犅狅狋犪狀犻犮犪犅狅狉犲犪犾犻犗犮犮犻犱犲狀狋犪犾犻犪犛犻狀犻犮犪,2009,29(2):338343.
[24] wêH,=¥b.pHu·%8Œ7sxyE‘ˆ‰zŽ
[J].R%&,1998,24(4):509512. MACC,HONGFS.EffectofpHonseedgerminationand seedlingsgrowthandmetabolisminrape[J].犃犮狋犪犃犵狉狅狀狅犿犻 犮犪犛犻狀犻犮犪,1998,24(4):509512. [25] BEAULIEU W T,PANACCIONEDG,HAZEKAMPCS, 犲狋犪犾.Differentialalocationofseedborneergotalkaloidsdur ingearlyontogenyofmorningglories(犆狅狀狏狅犾狏狌犾犪犮犲犪犲)[J]. 犑狅狌狉狀犪犾狅犳犆犺犲犿犻犮犪犾犈犮狅犾狅犵狔,2013,39(7):919930. [26] MILESCO,LANEGA,diMENNAME,犲狋犪犾.Highlevels ofergonovineandlysergicacidamideintoxic犃犮犺狀犪狋犺犲狉狌犿 犻狀犲犫狉犻犪狀狊accompanyinfectionbyan犃犮狉犲犿狅狀犻狌犿likeendo phyticfungus[J].犑狅狌狉狀犪犾狅犳犃犵狉犻犮狌犾狋狌狉犪犾犪狀犱犉狅狅犱犆犺犲犿犻狊 狋狉狔,1996,44(5):12851290. [27] ZHANGXX,LICHJ,NANZHB,犲狋犪犾.犖犲狅狋狔狆犺狅犱犻狌犿犲狀 犱狅狆犺狔狋犲increases犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊 (drunkenhorse grass)resistancetoherbivoresandseedpredators[J].犠犲犲犱 犚犲狊犲犪狉犮犺,2012,52(1):7078. [28] ›IJ,X<}.IJKELMEŠzCD…8[J].Ee%
&
,2007,27(6):25322546.
GAOJH,NANZB.Areviewofbioprotectivealkaloidsof
grassfungalendopytesymbioses[J].犃犮狋犪犈犮狅犾狅犵犻犮犪犛犻狀犻犮犪,
2007,27(6):25322546.
[29] ˆK),_ L,TUV,Ÿ.†‡EŠ?vwJKELM NEO°z7³à [J].Jb%&,2010,19(6):215221. DAILY,HUANGX,LICJ,犲狋犪犾.Spatialvariationofergotal kaloidsindrunkenhorsegrassinfectedby犖犲狅狋狔狆犺狅犱犻狌犿犵犪狀 狊狌犲狀狊犲[J].犃犮狋犪犘狉犪狋犪犮狌犾狋狌狉犪犲犛犻狀犻犮犪,2010,19(6):215221. [30] ZHANGXX,LICHJ,NANZHB.Effectsofcuttingfre quencyandheightonalkaloidproductioninendophyteinfec teddrunkenhorsegrass(犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊)[J].犛犮犻犲狀犮犲 犆犺犻狀犪犔犻犳犲犛犮犻犲狀犮犲狊,2011,54(6):567571. [31] MU#,TëN,Ÿ.ŸÔªuvwJKELMNEO†‡E Š‹ŒzŽ
[J].Jb-%,2014,31(5):844849.
HUCX,LIXZ,犲狋犪犾.Efectsoftryptophanonergotalkaloidscon
centrationsof犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊犖犲狅狋狔狆犺狅犱犻狌犿犵犪狀狊狌犲狀狊犲as
sociation[J].犘狉犪狋犪犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲,2014,31(5):844849.
[32] + O,ÌÍÎ,!ûþ,Ÿ.º»MYbPªuvwJxyE‘
YEŠ‹ŒzŽ [J].Jb-%,2014,31(11):21132118. WANGP,ZHANGXX,ZHAOXJ,犲狋犪犾.Effectsofethphon andsalicylicacidongrowthandergotalkaloidsconcentrations of犃犮犺狀犪狋犺犲狉狌犿犻狀犲犫狉犻犪狀狊seedlings[J].犘狉犪狋犪犮狌犾狋狌狉犪犾犛犮犻 犲狀犮犲,2014,31(11):21132118. [33] ZHOULY,LICHJ,ZHANGXX,犲狋犪犾.Effectsofcold shocked犈狆犻犮犺犾狅infected犉犲狊狋狌犮犪狊犻狀犲狀狊犻狊onergotalkaloid accumulation[J].犉狌狀犵犪犾犈犮狅犾狅犵狔,2015,14:99104. [34] GERHARDSN,NEUBAUERL,犲狋犪犾.Biosyntheticpathwaysof ergotalkaloids[J].犜狅狓犻狀狊,2014,6(12):32813295. ( !" : #%

027 ! " # $ % & 36*

  鄂ICP备06018747号 Copyright @ 2017
  植物通 All Rights Reserved
  Email:23198511@qq.com
回顶部
51La