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Growth and Physiological Characteristics of Abelmoschus esculentus Plantlets in vitro under Different Quality Lights

不同LEDs复合光质对秋葵组培苗生长和生理特性的影响



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,2016,36(5):0996-1003
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犌狉狅狑狋犺犪狀犱犘犺狔狊犻狅犾狅犵犻犮犪犾犆犺犪狉犪犮狋犲狉犻狊狋犻犮狊狅犳犃犫犲犾犿狅狊犮犺狌狊犲狊犮狌犾犲狀狋狌狊
犘犾犪狀狋犾犲狋狊犻狀狏犻狋狉狅狌狀犱犲狉犇犻犳犳犲狉犲狀狋犙狌犪犾犻狋狔犔犻犵犺狋狊
LIHuimin
(ColegeofLifeScience,AnhuiScienceandTechnologyUniversity,Fengyang,Anhui233100,China)
犃犫狊狋狉犪犮狋:Shootapicalbudcuttingsofokracultivar‘Qingfu’犻狀狏犻狋狉狅 (1.0cm)weretransplantedonMS
basalmediumsupplementedwith0.05g·L-1naphthaleneaceticacid(NAA)andculturedundereightdif
ferentqualitylights:fluorescentlamp(CK),fourmixtureblueplusredlightemittingdiodes(BR3∶1,
BR1∶1,BR1∶3,BR1∶4LEDs),blue,redplusyelow(BRY=1∶3∶1)LEDs,blue,redplusgreen(BRG=
1∶3∶1)LEDs,blue,redplusfarred(BRFR=1∶3∶1)LEDs.Theobjectiveofpresentstudywasto
determinetheeffectsofdifferentLEDslightsonthegrowthandphysiologicalcharacteristicsofokra
(犃犫犲犾犿狅狊犮犺狌狊犲狊犮狌犾犲狀狋狌狊)plantlets,andscreenforoptimalqualityofokraplantlets犻狀狏犻狋狉狅inordertopro
videtheoreticalguidanceandtechnicalsupportofokrarapidpropagation犻狀狏犻狋狉狅.Theresultsshowed
that.(1)freshmass,drymass,rootlength,stemlengthandstemwidthwerethegreatestinplantlets
culturedunderBRYLEDslight;(2)rootactivityshowedsignificantlyhigherinplantletsculturedunder
differentlightsthanthatofCK,andthehighestwasunderBRYLEDs;Photosyntheticpigmentswerethe
highestinplantletsculturedunderBRYLEDs,andwhichwerealsohigherthanthatofothertreatments;
(3)theactivitiesofSOD,PODandCATwerethegreatestinplantletsculturedunderBRYLEDs,andthe
lowestwasthatofCK;(4)theconcentrationsofsolublesugar,sucrose,solublecarbonandstarchwere
thegreatestinseedlingsunderBRYLEDstreatment,folowedbyBRG,andthelowestunderCK;The
concentrationsofsolubleprotein,freeaminoacidandsolublenitrogenwerethegreatestinseedlingsunder
BRYtreatment,folowedbyBRG,andthelowestunderCK.Thepresentresultsindicatedthatlargerand
healthyplantletsandgreaterbiomassofokrawereproducedinthepresenceofBRY (1∶3∶1)LEDs
light.BRYlightsignificantlypromotedtherootactivity,photosytheticpigmentcontent,antioxidanten
zymeactivities,carbonandnitrogenmetabolismandaccumulationofphotosyntheticproductofokraplant
lets,whichwasthepreferredlightqualityfortheokra犻狀狏犻狋狉狅culturesystem.
犓犲狔狑狅狉犱狊:mixturequality;okra;plantlet;physiologicalcharacteristics;犻狀狏犻狋狉狅
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Table1 MajortechniqueparametersoflightspectralenergydistributionunderLED
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Lighttreatment
€j{ê
Lightspectraldistribution
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Peakvalue
λp/nm
ìíî
Halfwavewidth
Δλ/nm
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Lightintensity
/μmol·m-2·s-1
CK €Fluorescent 380~760 — 45
BR3∶1 „/…Blue/red(3∶1) 660/460 10~15 45
BR1∶1 „/…Blue/red(1∶1) 660/460 10~15 45
BR1∶3 „/…Blue/red(1∶3) 660/460 10~15 45
BR1∶4 „/…Blue/red(1∶4) 660/460 10~15 45
BRY „/…/ˆRed/blue/yelow(1∶3∶1) 660/460/590 10~15 45
BRG „/…/‰Red/blue/green(1∶3∶1) 660/460/530 10~15 45
BRFR „/…/‹…Red/blue/farred(1∶3∶1) 660/460/735 10~15 45
Ä

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22.97%、15.70%、13.33%、6.73%Š6.61%(犘<
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Table2 Thegrowthof犃.犲狊犮狌犾犲狀狋狌狊plantlets犻狀狏犻狋狉狅underdifferentqualitylights
€¤H
Lighttreatment
¥”¦
Freshmass/g
§”¦
Drymass/g
¨–
Rootlength/cm
©–
Stemlength/cm
©ª
Stemwidth/cm
CK 1.65f 0.13e 7.93g 8.67f 1.66f
BR3∶1 2.15e 0.17d 9.53e 10.11e 1.77d
BR1∶1 2.27d 0.19c 10.09d 10.17e 1.77d
BR1∶3 2.41c 0.22bc 10.27c 10.36c 1.81c
BR1∶4 2.35c 0.21bc 10.23c 10.27d 1.79c
BRY 2.68a 0.25a 11.27a 11.26a 2.13a
BRG 2.54b 0.23b 10.67b 10.46b 1.85b
BRFR 2.18e 0.18cd 8.85f 9.22e 1.71e
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Fig.1 Therootactivityof犃.犲狊犮狌犾犲狀狋狌狊plantlets
犻狀狏犻狋狉狅underdifferentqualitylights
‹BR1∶1、BR1∶4Š BR3∶1¤Höâ´&|,è
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Fig.2 Thephotosyntheticpigmentcontentof犃.犲狊犮狌犾犲狀狋狌狊plantlets犻狀狏犻狋狉狅underdifferentqualitylights
9995            89::’“LEDs†‡€”‚XYiF•G–ŠGH—ÅPtu
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Fig.3 TheSOD,PODandCATactivitiesof
犃.犲狊犮狌犾犲狀狋狌狊plantlets犻狀狏犻狋狉狅under
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