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作 者 ：韩 超1，徐晓立2

期 刊 ：西北植物学报 2016年 8期 页码：1594~1599

关键词：桉树；根系；石蜡切片；不定根发育；

Keywords:Eucalyptus, root, paraffin section, adventitious roots development,

摘 要 ：以巨尾桉‘GL9’、尾巨桉‘DH32 29’和尾边桉‘XF35’3种桉树无性系组培生根苗为材料，采用常规石蜡切片技术，对石蜡切片制作过程中的固定环节进行了优化，观察了桉树不定根的发育过程。结果表明：（1）采用FAA固定液固定材料，可获得染色清晰，组织完整的桉树根系切片。（2）‘GL9’和‘DH32 29’在生根诱导8 d后生出不定根，生根类型为皮部生根；‘XF35’在生根诱导12 d后生出不定根，生根类型为愈伤组织生根；‘GL9’不定根的根尖和根基处均有细胞旺盛分裂，‘DH32 29’不定根只在根尖有细胞旺盛分裂，‘XF35’不定根则只在其根基处有大量旺盛分裂的细胞。

Abstract:This paper optimized the fixing procedure of the routine paraffin section technology and observed the process of adventitious rooting development of three culture rooting seedlings of Eucalyptus clones ‘DH32 29’, ‘GL9’ and ‘XF35’. The results showed that: (1) FAA fixing solution was better with clear staining and tissue integrity root slice on Eucalyptus. (2) Adventitious roots of ‘DH32 29’ and ‘GL9’ originated after 8 days being induced and belongs to cortices rooting type. Adventitious roots of ‘XF35’ originated after 12 days being inducted and belongs to callus rooting type. Active division cells are in root tip and root foundation of ‘GL9’ adventitious root. Active division cells are only in root tip of ‘DH32 29’ adventitious root. Active division cells are only in root foundation of ‘XF35’ adventitious root.

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