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作 者 ：扈 顺1,刘果厚1*,周国栋2,韩春荣3,岳秀贤4

期 刊 ：西北植物学报 2013年 7期 页码：1373~1377

关键词：四合木；扦插生根；淀粉粒；蛋白质；

Keywords:Tetraena mongolica Maxim., cutting rooting, starch grains, proteins,

摘 要 ：利用高碘酸-锡夫试剂（PAS）—萘酚黄S对染法对四合木扦插不定根发生发育过程中淀粉粒和蛋白质分布的动态变化进行观察分析,为进一步揭示四合木不定根发生、发育机理提供解剖学依据。结果表明:（1）扦插后,淀粉粒在茎的木栓层和维管形成层之间的薄壁细胞内积累,至愈伤组织发育初期达到高峰,随着愈伤组织的继续发育,淀粉粒逐渐减少;（2）蛋白质在茎的木栓层和维管形成层之间的薄壁细胞内逐渐减少,至愈伤组织形成后,细胞内含少量蛋白质;（3）淀粉粒和蛋白质主要分布在分裂旺盛的胚性细胞、不定根原基细胞、不定根细胞及其附近细胞内。研究认为,蛋白质和淀粉粒为四合木不定根的形成和发育提供了物质和能量。

Abstract:In order to further understand the mechanism of adventitious root formation and development of Tetraena mongolica Maxim.,we studied the dynamical changes of starch grain and protein accumulation by PAS (Periodic acid-Schiff)/S (Naphthol yellow S) in the origin and development of adventitious roots of T.mongolica Maxim..Results after cutting indicated that:（1）Starch grains were accumulation in the parenchyma cells between phellogen cambium and vascular cambium in stem.Starch grains achieved height value was reduced gradually with callus development continued in the callus development primary.(2)Proteins were reduced in the parenchyma cells between phellogen cambium and vascular cambium in stem.Parenchyma cells contains a little of proteins after callus formed.(3)Starch grains and proteins were mainly distributed in vigorous dividing cells,organ primodium,and adventitious roots cells and nearby cells.Protein and starch provided material and energy to formation and development of adventitious roots of T.mongolica Maxim..

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