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作 者 ：王 荣1，韩旭思2，高 飞2*，周宜君2，姜双林1

期 刊 ：西北植物学报 2012年 3期 页码：465~470

关键词：盐芥；蛋白质组；干旱胁迫；生物质谱；

Keywords:Thellungiella halophila, proteome, drought stress, mass spectrometry,

摘 要 ：盐芥是新兴起的植物非生物逆境研究模式植物，研究盐芥叶片蛋白质组对于干旱胁迫的响应，以推进对植物干旱耐受机制的认识。该研究应用双向电泳技术分析了干旱胁迫对于盐芥叶片蛋白质组的影响，结果共鉴定了63个干旱胁迫差异表达蛋白，包括丰度上调的31个，新出现的蛋白点14个，丰度下调的15个，消失的蛋白点3个。应用生物质谱分析技术确定了包括硫氧还蛋白，铁蛋白-1和凝集素在内的9个干旱胁迫响应蛋白的身份，对这些干旱胁迫响应蛋白的功能分类分析表明，盐芥的耐旱机制可能涉及自由基清除能力的增强、能量代谢的调整以及光合作用的维持。

Abstract:Drought is one of the most severe environmental factors that may impair growth and development of plant and decrease crop productivity.Thellungiella halophila,an Arabidopsis-related halophyte is an emerging model used for plant abiotic stress tolerance research.The proteome investigation of the response of drought stress in T.halophila leaf will advance the understanding on how plant adapt to drought stress.In this study,a proteomic study based on two-dimensional gel electrophoresis is performed in order to analyze the drought stress response of T.halophila.Totally,63 differentially displayed spots are determined,including 31 up-regulated,14 newly-appeared,15 down-regulated and 3 disappeared protein spots.Nine differentially displayed spots,including thioredoxin,ferritin-1 and lectin are identified by mass spectrometry analysis.Function classification analysis of the nine identified proteins reveals that the drought tolerance of T.halophila is achieved,at least partly,by enhancing ROS scavenging,adjusting energy metabolism and maintaining photosynthesis function.

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