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作 者 ：徐丽娟，张蜀宁*，郑于莉，张 宇，侯喜林

期 刊 ：西北植物学报 2013年 6期 页码：1239~1244

关键词：不结球白菜；秋水仙素；响应；形态学；动态变化；

Keywords:Non-heading Chinese Cabbage, colchicine, response, morphology, dynamic change,

摘 要 ：以二倍体不结球白菜‘依伶’为材料，用0.2%秋水仙素溶液处理子叶期生长点，研究不结球白菜在形态学上对秋水仙素诱导的响应及动态变化。结果表明：（1）‘依伶’对秋水仙素诱导的响应均以幼苗期前4片真叶中出现变异特征叶的形式表现，诱导处理15 d后出现了圆盘状、豆瓣状、棒状3种响应类型；处理30 d后，以上3种类型分别分化出长勺状、花状、圆锥状类型。（2）分化前后3种响应类型受秋水仙素抑制程度不同导致生长速率各不相同，生长速率的顺序分别为：圆盘状＞豆瓣状＞棒状，花状＞长勺状＞圆锥状，6种响应类型的特征叶逐渐萎蔫死亡，继续生长的其他.叶生长正常。（3）豆瓣状、圆盘状、长勺状、棒状、花状、圆锥状6种叶片形态响应类型于处理当代选株中四倍体加倍率依次分别为50.00%、41.67%、25.00%、16.67%、8.33%、0.00%。研究表明，早期筛选宜选用圆盘状、豆瓣状变异植株。

Abstract:With diploid non-heading chinese cabbage ‘Yiling’ as material,0.2% colchicines was dropped in growth point of cotyledon stage.We studied non-heading Chinese cabbage response on colchicine induced and dynamic change in morphology.The results showed that:(1)‘Yiling’ response on colchicine induced is that variation of leaf appear in the first four leaves of seedling stage.After 15 days of treatment,we observed three response types:disc character,bean character and coliform character.After 30 days of treatment,disc character,bean character and coliform character is developed macro-scoop character,flower character and conical character respectively.(2)The above six response types have different degrees of inhibition of seedling stage:disc character＞bean character＞coliform character,flower character＞macro-scoop character＞conical character;the variation characteristic leaf wilting and died gradually,other variation leaves grow normally.(3) disc character,bean character,coliform character,macro-scoop character,flower character and conical character,Rate of tetraploid of six responsed types above is 50.00%,41.67%,25.00%,16.67%,8.33% and 0.00%,repectively,disc character and bean character should be used for early screening.

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