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作 者 ：廖菊够1,康洪梅1,代瑾然1,姚 恒2,马文广2,陈穗云1*

期 刊 ：西北植物学报 2013年 1期 页码：11~16

关键词：迪勃纳氏烟草；大孢子发生；小孢子发生；雌配子体；雄配子体；

Keywords:Nicotiana debneyi, megasporogenesis, microsporogenesis, female gametophyte, male gametophyte,

摘 要 ：采用卡宝品红染色压片和石蜡切片法对迪勃纳氏烟草(Nicotiana debneyi)大小孢子发生和雌雄配子体发育过程进行了研究。结果发现:（1）迪勃纳氏烟草为两性花,其小孢子发生和雄配子体发育早于大孢子。（2）迪勃纳氏烟草小孢子发生和发育过程基本正常,减数分裂过程有少数细胞出现滞后染色体、染色体断片和染色体桥等异常现象；其胞质分裂为同时型,四分体为十字交叉型和正四面体型,成熟的花粉粒为2细胞型。（3）迪勃纳氏烟草为2室子房,中轴胎座,倒生胚珠,胚珠多数,胚囊发育为蓼型,大孢子发生和发育过程未观察到异常现象。

Abstract:The megasporogenesis,microsporogenesis and the development of female and male gametophyte of Nicotiana debneyi were studied by carbol fuchsin staining and paraffin sectioning.The results showed that:(1)N.debneyi is hermaphrodite flower and the microspore develops earlier than megaspore.(2)Microsporogenesis and development of male gametophyte of N.debneyi are normal but there are chromosome bridges,chromosome fragment,laggard chromosome in a few cells.The meiosis in microspore of N.debneyi is simultaneous cytokinesis and the tetards are decussate or tetrahedral types.The mature pollens belong to 2 cell type.(3)N.debneyi is ovary 2-locular,axile placenta,ovules numerous and polygonum type of mature embryo sac.Megasporogenesis and the development of female gametophyte of N.debneyi are normal.

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