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作 者 ：宋丽丽，贺热情，黄星群，谢敏敏，赵小英*，刘选明

期 刊 ：西北植物学报 2012年 4期 页码：645~650

关键词：甘蓝型油菜；BnCYP78A8；Genome walking；序列分析；

Keywords:Brassica napus, BnCYP78A8, Genome walking, sequence analysis,

摘 要 ：采用Genome walking方法，首次克隆到甘蓝型油菜BnCYP78A8的基因组序列，根据基因特异性引物克隆到其编码序列。基因组序列长1 679 bp，有1个内含子和2个外显子。编码序列长1 605 bp，编码534个氨基酸。序列比对分析表明，其氨基酸序列与拟南芥细胞色素P450单加氧酶基因（AtCYP78A8）的相似度高达88%。生物信息学分析显示，该蛋白含有1个细胞色素P450特有的亚铁血红素配合基结合位点和1个跨膜结构域。实时荧光定量PCR分析结果表明，BnCYP78A8在甘蓝型油菜的各个器官组织均有表达，根中表达量最高，表明该基因可能参与根的生长发育。

Abstract:A total of 1 679 bp genomic DNA of BnCYP78A8 was first cloned from Brassica napus using Genomic walking method and then the coding sequence was cloned using special primers.The genomic DNA sequence is 1 679 bp,containing one intron and two exons.The coding sequence is 1 605 bp，coding for 534 amino acids.Sequence alignment of amino acid showed that BnCYP78A8 shared high identity （88%) with AtCYP78A8 in Arabidopsis thaliana.Bioinformatics analysis indicated that BnCYP78A8 protein contain one cytochrome P450 cysteine heme-iron ligand signature and one transmembrane domain.The real-time PCR results demonstrated that BnCYP78A8 expressed in all indicated tissues of Brassica napus,and its expression in root was higher than that in other tissues,suggesting that BnCYP78A8 might be involved in root growth.

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