作 者 ：曹永慧1，周本智1，张汝民2，陈双林1
期 刊 ：西北植物学报 2012年 3期 页码：525~531
Keywords:Illicium lanceolatum, light condition, chlorophyll fluorescence, photosystem Ⅱ,
摘 要 ：以天目山披针叶茴香（Illicium lanceolatum）4年生栽培幼苗为对象，经不同光环境（自然全光照，50%光照和20%光照）处理后，采用雅欣理1611植物效率仪测试，并进行光系统Ⅱ（PSⅡ）快速叶绿素荧光诱导动力学分析(JIP-test)，以探讨其光适应机制，为高莽草酸含量植株高效栽培技术提供理论依据。结果显示：（1）随着遮光程度增加，披针叶茴香叶片叶绿素a（Chl a）、叶绿素b（Chl b）和总叶绿素含量（Chl（a+b））呈上升趋势，均与全光照存在显著差异；Chl a/Chl b值分别降低了22.92%和31.56%，均与全光照差异极显著。（2）随遮光程度增强，PSⅡ最大光能转换效率（Fv/Fm）降低，50%和20%光照处理的Fv/Fm值分别比全光照下降了1.34%和2.79%，且20%光照处理与全光照差异显著。（3）随遮光程度增强，50%光照和20%光照处理叶片单位面积光合机构含有的反应中心数目（RC/CSo）分别比全光照减少了2.94%和13.63%，单位反应中心以热能形式耗散的能量（DIo/RC）分别增加了2.2%和62.9%。研究表明，50%光照处理下披针叶茴香叶片用于光合电子传递的能量占吸收光能的比例变化不显著，而在20%光照处理则显著降低，即50%遮光环境有利于披针叶茴香提高光能利用效率，促进其生长和增加生物量积累。
Abstract:Illicium lanceolatum is a unique plant in traditional Chinese medicine.The shikimic acid,extracted from I.lanceolatum,has functions of anti-inflammatory,analgesic and inhibiting platelet aggregation,arterial and venous thrombosis and cerebral thrombosis.It can also function as an intermediate for anti-virus and anti-cancer drugs.For example,it is a vital element of Oseltamivirphosphate for anti-Avian Influenza.Because of this,I.lanceolatum and other species in this genus have been given an unprecedented attention and have potential for further development and utilization.The primary objective of the study was to examine the physiological and biochemical foundation of growth and biomass accumulation of I.lanceolatum seedlings and to provide theoretical guidance for regular cultivation.The chlorophyll fluorescence kinetic technique was used in this study.The kinetic parameters of fast chlorophyll fluorescence were measured for 4-year-old I.lanceolatum seedlings in Zhejiang Province of China,under different light shading treatments (0%,50% and 80% shading) using a plant efficiency analyzer (PEA) and JIP-test,to provide the scientific bases for developing a high efficiency artificial cultivation techniques of I.lanceolatum with high content of shikimic acid.The results showed that:(1)Chlorophyll a (Chl a),chlorophyll b (Chl b) and total chlorophyll content (Chl(a+b)) increased with the increasing of shading degree and were significantly different with the full light (P<0.05).The value of Chl a/Chl b in two shading treatments decreased by 22.92% and 31.56% and had significant differences with full sunlight (P<0.01).(2)The maximal photochemical efficiency of photosystem Ⅱ(Fv/Fm) decreased as the shading level increased.(3)Comparing with the control (100% light),the ratio of Fv/Fm for 50% and 80% shading treatments decreased by 1.34% and 2.79%,respectively.Comparing with the control,the density of reaction centers (RC/CS) decreased by 2.94% and 13.63% for 50% and 80% shading treatments,respectively and the energy dissipation per RC(DIo/RC) increased by 2.2% and 62.9%,respectively.ANOVA analysis showed that actual light use efficiency,the fraction of absorbed light in photochemistry (P),for I.lanceolatum leaf under 50% shading exhibited no significant difference from the control (P>0.05).However,for I.lanceolatum leaf under 80% shading,there was a significant decrease of the fraction of absorbed light in photochemistry (P<0.01).We concluded that I.lanceolatum seedlings under 50% shading could enhance the actual light use efficiency and help to improve biomass accumulation and content of shikimic acid of I.lanceolatum plants.