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作 者 ：张学军1,2,张永兵1,2,张 龑3,王登明1,2,伊鸿平1,2*

期 刊 ：西北植物学报 2013年 2期 页码：261~265

关键词：甜瓜；蔓枯病；抗病基因；ISSR分子标记；

Keywords:Cucumis melo L., gummy stem blight, disease resistance gene, ISSR marker,

摘 要 ：以甜瓜抗病资源PI511890和感病自交系‘白皮脆’及其F₁、BC₁P₁、BC₁P₂、F₂群体为材料,苗期接种蔓枯病菌（Didymella bryoniae）进行遗传分析。结果表明:（1）对甜瓜苗期蔓枯病菌接种鉴定结果显示,抗源PI511890的抗病基因Gsb-3由显性单基因控制。（2）利用集团分离分析及ISSR分析技术,引物ISSR-100扩增出的多态性条带与Gsb-3表现连锁关系,该多态性片段大小为900 bp。（3）统计ISSR-100在182个F₂单株上的多态性,并用JoinMap 4.0软件分析结果显示,ISSR-100与Gsb-3遗传连锁距离为8.3 cM,定名为ISSR-100₉₀₀。研究认为,ISSR-100₉₀₀可作为甜瓜抗蔓枯病分子育种的备选分子标记。

Abstract:Segregating population consisting of F₁,BC₁P₁,BC₁P₂ and F₂ were generated by crossing the resistant inbred line PI511890 with the susceptible inbred line‘Baipicui’.The resistance of segregating population and parental lines were evaluated by inoculating spore isolates of gummy stem blight at seeding stage.The results indicated that:(1)The resistance to gummy stem blight in PI511890 is controlled by a single dominant gene.(2)Using bulked segregation analysis,an unique 900 bp fragment was amplified in resistant materials with the primer ISSR-100.It was confirmed that the marker ISSR-100₉₀₀ was linked to the gene Gsb-3.(3)The polymorphic patterns of ISSR-100 in the 182 individuals of F₂ were scored and then calculated by the software of JoinMap 4.0.The results showed that the genetic distance between ISSR-100 and Gsb-3 is 8.3 cM.This ISSR band indicated that the marker could be useful for marker assisted selection in melon breeding for gummy stem blight resistance.

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