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作 者 ：曹江玲，贺明阳，朱自果，王跃进*

期 刊 ：西北植物学报 2012年 5期 页码：859~865

关键词：中国野生毛葡萄；芪合成酶；白藜芦醇；表达分析；

Keywords:Chinese wild Vitis quinquangularis, stilbene synthase, resveratrol, expression analysis,

摘 要 ：采用RT-PCR技术克隆中国野生毛葡萄‘丹凤-2’芪合成酶基因，命名为VqDSTS1，并进行序列及表达模式分析。结果表明:VqDSTS1基因cDNA编码区全长为1 179 bp，GenBank登录号为JQ342086,编码392个氨基酸；氨基酸序列分析表明，VqDSTS1含有芪合成酶基因家簇的特征识别序列‘IPNSAGAIAGN’和‘GVLFGFGPGLT’；序列比对显示,VqDSTS1与其他葡萄种质的芪合成酶氨基酸序列一致性在95.2%~98.7%之间；半定量RT-PCR分析表明，VqDSTS1受白粉病诱导表达，呈双峰模式。为进一步研究中国野生毛葡萄‘丹凤-2’芪合成酶基因家族的表达及功能分析提供了基础。

Abstract:VqDSTS1,was isolated by reverse transcription-polymerase chain reaction (RT-PCR) from Chinese wild Vitis quinquangularis accession ‘Danfeng-2’,and the sequence and expression model then was analyzed.The results suggested the full length cDNA sequence of VqDSTS1 was 1 179 bp,and the accession number of VqDSTS1 was JQ342086,and encoded 392 amino acid residues.Detailed analysis of the amino acid sequence revealed that VqDSTS1 contained “IPNSAGAIAGN” and “GVLFGFGPGLT” which could be found in all stilbene synthase gene family.With alignment of amino acid sequences,VqDSTS1 shared highly identity between 95.2%~98.7% with the stilbene synthase of other Vitis.From the analysis of semi-quantitative RT-PCR,it was found that the VqDSTS1 expression is introduced by powdery mildew with twin-peak model.This work would make a foundation to investigate the function of stilbene synthase gene family from Chinese wild Vitis quinquangularis accession ‘Danfeng-2’.

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