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作 者 ：李 珍，王东浩，姚 伟，陈玉芹，王喆之*

期 刊 ：西北植物学报 2013年 7期 页码：1285~1291

关键词：环阿屯醇合酶；基因克隆；丹参；表达分析；

Keywords:cycloartenol synthase, gene clone, Salvia miltiorrhiza, expression analysis,

摘 要 ：以丹参cDNA为模板，克隆了丹参环阿屯醇合酶(cycloartenol synthase,CAS)基因的cDNA序列(SmCAS)，对其序列进行生物信息学分析，并采用实时荧光定量PCR方法研究了该基因在丹参不同器官及不同胁迫处理下的表达模式。结果显示:该基因全长2 346 bp，包含2 271 bp开放阅读框，编码756个氨基酸。预测其编码蛋白分子量为86.16 kD，具有氧化鲨烯环化酶超家族典型的DCTAE结构域和QW结构域。该基因推测的氨基酸序列与人参、田七、积雪草、甘草、拟南芥的相似性分别为83%、84%、83%、81%和80%。SmCAS基因在丹参根、茎、叶、花中均有表达，在花中表达量最高;而且SmCAS基因能够响应ABA、低温和干旱的诱导。

Abstract:A full length cDNA of cycloartenol synthase gene (SmCAS) was cloned from Salvia miltiorrhiza Bunge.We analyzed the sequence and structure characteristics of its encoded protein,and its expression patterns in the different organism and under different stress by quantitative RT-PCR.The full length cDNA is 2 346 bp,which includes 2 271 bp ORF and encodes 756 amino acid residues with a putative molecular mass of 86.16 kD.SmCAS has a typical conserved DCTAE motif and QW motif of oxidosqualene cyclase.Bioinformatics analysis showed that SmCAS had 83%,84%,83%,81% and 80% similarity with P.notoginseng,P.ginseng,C.asiatica,G.glabra and Arabidopsis thaliana,respectively.SmCAS expressed significantly in different tissues and had the highest transcript profile in flowers.It could be positive induced by ABA,lower temperature and drought,while its expression was inhibited by MeJA.

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