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作 者 ：吴建明1，李杨瑞1,2，杨丽涛2，方锋学1，李 强2

期 刊 ：西北植物学报 2012年 10期 页码：1977~1982

关键词：赤霉素；甘蔗；cDNA-AFLP；差异表达；

Keywords:gibberellin, sugarcane, cDNA-AFLP, differential expression,

摘 要 ：以‘新台糖22号’甘蔗为材料，在其伸长初期以200 mg/L的赤霉素进行叶面喷施处理，对照喷清水，分别提取对照和处理（0 、6 、12、24 h）的总RNA进行等量混合，获得2种不同处理的样品池，采用cDNA-AFLP技术对赤霉素诱导下甘蔗节间伸长的差异表达进行分析。结果显示：（1）通过169对引物组合的选择性扩增，共得到了约11 000条大小在50~700 bp的cDNA片段，获得186条差异条带。（2）经反向Northern杂交，于结果显示阳性的TDFs中选取56个片段进行克隆和序列分析，获得56条大小在80～500 bp的差异条带。（3）经BLAST分析，按功能可将获得的56条TDFs分为7类，分别为能量与代谢相关基因、未知功能蛋白、未知基因、植物抗性相关基因、细胞壁生物合成与修饰相关基因、信号传导相关基因和转录因子相关基因。该研究获得了一些与甘蔗节间伸长相关的差异基因片段，为进一步研究甘蔗节间伸长的分子机理奠定了基础。

Abstract:In this paper,we used cDNA-AFLP to isolate differentially expressed genes in sugarcane induced by GA₃.Sugarcane plants (Saccharum officinarum cv.ROC22) which were sprayed with the concentration of 200 mg·L^-1 gibberellin at early elongation stage as treatment,while sprayed with distilled water as control.(1)One hundred and eighty six bands of differential expression genes were obtained by 169 pairs of selective amplification primers.After reverse Northern blot analysis,56 positive TDFs were selected for sequencing.(3)BLAST analysis showed the genes related to energy and metabolism (6),unknown functional protein (17),plant resistance (1),cell wall biosynthesis and modification (1),signal transduction (2),transcription factor (1) and others (28).Our result provides reference for investigating the molecular mechanism of sugarcane stem elongation.

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