Abstract:Based upon the plasmid pSVBV-E3 containing the full-length Strawberry vein banding virus (SVBV) genomes, infectious clone of SVBV was constructed. Fragments of 0.5-mer SVBV and 1.0-mer SVBV were obtained by digesting plasmid pSVBV-E3 with restriction enzymes, respectively, and then sequentially ligated to the plant expression vector pBINPLUS to generate the infectious clone pBIN-1.5SVBV. Plasmid pBIN-1.5SVBV was transformed into Agrobacterium tumefaciens, and then innoculated onto Fragaria vesca and 4 species of Nicotiana plants to validate its infectivity, respectively. The result showed that typical vein banding and yellowing symptoms appeared on F. vesca plants inoculated with SVBV infectious clone 8 weeks later, and cp gene of SVBV could be detected from symptomatic F. vesca plant by PCR, and the presence of SVBV genome was further confirmed by Southern blot. In contrast, no symptoms could be observed on 4 species of Nicotiana plants inoculated with SVBV infectious clone 8 weeks later, and cp gene of SVBV could not be detected by PCR, either. Taken together, successful establishment of infection of SVBV in F. vesca through agroinoculation of the infectious clone would provide a basis for further study the pathogenic mechanism of SVBV in F. vesca.