Abstract:The internal structure of mature Tuber indicum ascocarps were observed by means of scanning electron microscope. The result further confirmed that, the white veins covered on the cross section of mature T. indicum ascocarps were composed of sterile paraphysis, while the dark veins which were to be the gleba were the fertile hyphostroma intertwined and surrounded by paraphysis, these white and dark veins constituted the maze veins on the cross section of T. indicum ascocarps; the developing uniform asci surrounded by plenty of intertwined ascogenous hypha and paraphysis could be able to be observed in the gleba, forming a thickly dotted tiny honeycomb like structure; the ascospores distributed free in asci, densely ornamented with stings on the epispore of fully mature spores, slightly having a crook on the tip of sting. The size of ascospores in an ascus was related to the number of ascospores contained in this ascus, the more ascospores contained in an ascus, the smaller the ascospores would be. The internal anatomy structure observation of T. indicum ascocarps by means of scanning electron microscope in great detail can provide reliable and visualized accordance for classifying of Tuber and understanding the internal characteristics of Tuber ascocarps systematically, as well as forming the foundation for the psychological study of Tuber ascocarps.