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作 者 ：梁振普，宋小凤，张小霞，许锋，邵新峰

期 刊 ：植物病理学报 2012年 42卷 5期 页码：486-496

关键词：杨树黄叶病；蛋白质组；双向电泳；质谱；

Keywords:yellow-leaf disease of Populus, proteomics, two dimensional electrophoresis(2-DE), mass spectrometry,

摘 要 ：采用双向电泳和MALDI-TOF-TOF质谱联用技术，对近年来河南省的新生黄叶病害杨树进行了差异蛋白质组学研究。经图谱分析，在叶片、韧皮部和根部分别获得了98个（61个上调，37个下调）、90个（42个上调，48个下调）和41个（19个上调，22个下调）差异蛋白点。对其中差异表达1.5倍以上的61个蛋白点进行了质谱鉴定，58个蛋白质点鉴定成功，这些蛋白点可归类于26种蛋白质。这些蛋白包括参与光合作用和与抗性相关的蛋白。研究结果有助于揭示病害的发生机理，铁蛋白的鉴定结果对病因的确定具有重要参考价值。

Abstract:Two dimensional electrophoresis (2-DE) and MALDI-TOF-TOF mass spectrometry were used to analyze the proteomic changes of yellow-leaf diseased Populus in Henan Province. A total of 98 (61 up-regulated, 37 down-regulated), 90 (42 up-regulated, 48 down-regulated) and 41 (19 up-regulated, 22 down-regulated) differentially expressed protein spots were identified from leaf, phloem, root, respectively. 61 of these spots, with differential expression of 1.5 times or above, were further determined by MALDI-TOF-TOF and 58 sequences were finally obtained. These determined spots were classified into 26 proteins. These proteins were related to photosynthesis and resistance. The results, especially identification of ferritin, were of value for further investigation of the pathogenesis of yellow-leaf disease in Populus.

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