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作 者 ：贾笑英  张金文  王蒂

期 刊 ：西北植物学报 2006年 4期

关键词：glgC基因；克隆；原核表达；载体构建；

Keywords:glgC gene, cloning, prokaryotic expression, vector construction,

摘 要 ：以大肠杆菌BL21染色体DNA为模板,根据glgC基因的全序列设计了1对引物,在优化的PCR反应条件下扩增出了glgC基因片段,测序结果显示该片段大小为1296 bp,编码432个氨基酸残基。将该基因克隆到原核表达载体pET-28a-c( )中,重组载体pET-glgC转化至大肠杆菌BL21(DE3),经IPTG诱导后,SDS-PAGE电泳鉴定,得到了与理论推算的glgC基因表达产物分子质量(约53 kD)相符的特异蛋白条带。

Abstract:A pair of primers were designed by using genomic DNA of E.coli BL21 as the template and according to the whole sequence of glgC gene,then a glgC fragments were amplified with the pair of primers under optimized PCR conditions and the sequencing of the fra

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