Abstract:The tender stems of Malus komarovii were used as explant and the suitable medium compositions were screened through uniform design experiments. The results showed that tissue culture of M. komarovii required different kinds of culture medium in different phases. SH+TDZ 2.45 mg·L-1+NAA 0.06 mg·L-1 for shoots regeneration immediately at base of tender stem, the rate of induction was 98.8%; 1/4 SH+KT 0.35 mg·L-1+NAA 0.05 mg·L-1 for rooting, the rate of rooting was more than 97%; 1/8 SH+ABA 2.05 mg·L-1+KT 0.50 mg·L-1 for germplasm preservation in vitro, the rate of dormancy was 95.5%. These plant materials could be maintained for 39 months by the methods of promoting dormancy and low nutrients at normal temperature.