---

作 者 ：翟学杰,董凤祥,张日清,王贵禧,梁丽松

期 刊 ：林业科学研究 2009年 22卷 6期 页码：753-757

关键词：杂交榛；花粉；萌发；胼胝质；培养基；

Keywords:Corylus heterophylla Farnch.×C.avellana L., pollen, germination, callose, medium,

摘 要 ：采用固体培养基研究了不同浓度的蔗糖、硼和钙配比对杂交榛花粉萌发及花粉管生长的影响。结果表明:蔗糖、硼酸浓度间对花粉萌发有显著影响,钙浓度间对花粉萌发的促进作用不明显;蔗糖150 g·L^-1、硼酸100 mg·L^-1时最适宜花粉管伸长;蔗糖的缺乏会导致胼胝质在花粉管顶端积累,使其生长停滞,缺硼时胼胝质在花粉管内分布正常。杂交榛花粉适宜的培养基为:蔗糖150 g·L^-1+硼酸100 mg·L^-1+氯化钙100 mg·L^-1+琼脂10 g·L^-1。

Abstract:Solid medium was using to study the effect of sugar, boron, calcium on pollen germination and pollen tube growth of Corylus heterophylla Farnch.×C.avellana L.. The results showed that: different concentrations of sucrose and boric acid had significant impact on pollen germination, but different concentrations of calcium had no significant impact on pollen germination. It was most appropriate for tube elongation when sugar 150 g·L^-1 and boric acid 100 mg·L^-1. Lack of sucrose would lead to the accumulation of callose in top of pollen tube and hold back its growth. The distribution of callose was regular in pollen tube when the medium lacked boric. The appropriate medium for pollen germination of C.heterophylla×C.avellana was sugar 150 g·L^-1 + boric acid 100 mg·L^-1 + CaCl₂100 mg·L^-1 + agar 10 g ·L^-1.

---