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作 者 ：张 一,储德裕,金国庆,周志春,秦国峰

期 刊 ：林业科学研究 2009年 22卷 6期 页码：772-778

关键词：马尾松；ISSR；遗传多样性；杂交育种；

Keywords:Pinus massoniana, ISSR, genetic diversity, crossing breeding,

摘 要 ：利用12个ISSR标记分析马尾松1代育种群体中78个不同产地来源的优良亲本遗传变异,发现基于ISSR标记扩增的多态位点百分率、Nei‘s基因多样性指数和Shannon信息多样性指数均较高,表明马尾松1代育种亲本的整体遗传多样性水平较高。78个亲本之间遗传距离变化范围为0.115 - 0.776,平均遗传距离为0.371。亲本之间的ISSR遗传距离与亲本产地的纬向地理距离关系密切,纬度相差较大的亲本间ISSR遗传距离相对较大。利用部分参试亲本进行测交系交配并分析其中的优良杂交组合,发现大部分优良组合父本、母本产地的纬向地理距离较远,同时亲本间ISSR遗传距离也较大。根据本研究结果,应优先选择来自纬度差异较大的不同种源区、同时ISSR遗传距离也较大的优树作为高世代育种亲本,不仅能有效维持高世代育种群体较高的遗传多样性,也利于创制更多强优势杂交组合,提高杂种优势利用程度。

Abstract:By using 12 selected ISSR (inter-simple sequence repeat)markers, 78 first generation parents from different provenances of Pinus massoniana were assessed to detect their genetic differences. It was found that the percentage of population polymorphic loci, the Neis genetic diversity and the Shannons information diversity index were high, which indicated that the genetic variation of the first generation breeding population was at high level.The genetic distances ranged from 0.115 to 0.776 with an average genetic distance of 0.371. The genetic distance between parents with large latitudinal distance was greater than that with small latitudinal distance. The geographical latitudinal distance between parents of most superior cross combinations was large, and the genetic distance of most parents were greater than 0.430, indicating the parents with both large latitudinal distance and great genetic distance had the largest potential to exhibiting heterosis. It was proposed that elite trees with both large genetic distance and large geographical latitudinal distance should be preferentially considered in the selection of parents and construction of advanced generation breeding population, to maintain high level of genetic variability and improve the genetic gain of breeding.

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