作 者 ：周珊;虞方伯;郭明;单胜道;邸玉翠;徐少娟;李松华;郑圣丰
期 刊 ：林业科学 2011年 47卷 9期 页码：114-118
关键词：竹炭 ；固定化 ；紫外诱变 ；假单胞菌 ；间甲酚 ；
Keywords:bamboo-carbon , immobilization , UV mutation , Pseudomonas sp. , m-cresol ,
摘 要 ：
用紫外光对假单胞菌株进行诱变,以竹炭为载体,将紫外诱变假单胞菌固定在竹炭上,用竹炭固定化紫外诱变假单胞菌处理间甲酚水样。考察竹炭固定化紫外诱变假单胞菌投加量和水样pH值对间甲酚去除的影响以及进水浓度随反应时间的变化关系,研究竹炭固定化紫外诱变假单胞菌去除间甲酚的反应动力学。结果表明: 相对于原菌株,菌株经紫外诱变后,生长周期缩短了6 h。经紫外照射120 s的假单胞菌可以在竹炭表面及内部孔隙形成明显菌胶团,诱变菌在竹炭上所成的生物量明显较未经诱变菌增加。竹炭固定化紫外诱变假单胞菌能有效地去除水样中间甲酚。竹炭固定化紫外诱变假单胞菌投加量和水样pH值影响到间甲酚的去除效果,pH值在4~6时,间甲酚的去除效果较好。20 g竹炭固定化紫外诱变假单胞菌处理100 mL初始浓度50,100,120,150,180 mg·L-1 间甲酚模拟水样42 h,去除率依次为90.9%,76.4%,72.9%,64.6%和49.7%。竹炭固定化紫外诱变假单胞菌对间甲酚的去除能较好地符合零级反应方程。
Abstract:
Pseudomonas sp. was mutated by ultraviolet (UV). Then the UV mutated Pseudomonas sp. was immobilized on bamboo-carbon to investigate its effect in the removal of m -cresol. The effects of various parameters, such as amount of immobilized mutant Pseudomonas sp. and pH, on m -cresol removal were examined. Meanwhile, the m -cresol removal kinetics and the relationship between concentration and reaction time were obtained. The results show that compared with wild strain, the growth cycle of the strain which is treated by UV mutation is shortened by 6 h. Pseudomonas irradiated by UV for 120 s can form obvious zoogloea on the bamboo-carbon surface area and inner pore, and the biomass generated by the mutant on the bamboo-carbon is significantly larger than that by the wild strain. Parameters such as immobilized biomass and pH affect the removal of m -cresol. The removal effect is better with pH at the range of 4 to 6. When 20 g of bamboo-carbon immobilized UV mutated Pseudomonas sp. is used to treat 100 mL of simulated m -cresol water sample, with its initial concentration being 50, 100, 120, 150, 180 mg·L-1 , removal effect is respectively 90.9%,76.4%,72.9%,64.6% and 49.7% after 42 h and the removal of m -cresol follows zero-order kinetics.
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