以新疆巩留县和新源县2个居群下的7个新疆野苹果群体当年生枝条为材料,应用EIS法对各群体抗寒性进行测定,并与电导率法进行对比分析。结果表明: 巩留和新源2个居群内群体间的半致死温度差异不显著,2个居群间的差异显著,巩留居群的半致死温度显著低于新源居群,证明巩留居群的抗寒性高于新源居群。电阻抗图谱法单-DCE模型中6个参数(τ,ψ,r,r1,re,ri)中,r1,re,τ 3个参数可以得出供试样品的半致死温度,其中r1,re所得的半致死温度与电导率法相关系数达到极显著水平,说明参数r1,re适用于新疆野苹果的抗寒性测定。电阻抗图谱法(EIS)与电导率法的抗寒性测定结果基本一致,但省时、不需温育,具有更大优越性。
Malus sieversii is an important germplasm resource, and valuable for genetic improvement and conservation of genetic diversity of apples. In this paper, one-year-old seedlings from 7 populations of Malus sieversii in Gongliu and Xinyuan of Xinjiang were used. Cold hardiness of the seedlings was determined by means of the EIS method in comparison with EL method. The results with different cold hardiness determination methods indicated that there was no obvious difference in the semilethal temperature within populations of Gongliu and Xinyuan. In contrast, there was significant difference in the semilethal temperature between the two populations. The semilethal temperature of Gongliu populations was significantly lower than that of Xinyuan populations. Thus, the cold resistance of Gongliu populations was higher than that of Xinyuan populations. Among the six parameters (τ,ψ,r,r1,re and ri) in the Single DEC model of electrical impedance spectroscopy, was obtained by three parameters (r1,re and τ) was able to be used t determine semilethal temperature of the tested samples. The semilethal temperature obtained by r1 and re was extremely significantly correlated with the conductivity method, suggesting that r1 and re could be used to determine the M. sieversii cold resistance. The cold hardiness estimated by the EIS method was basically similar to that, assessed by means of the traditional EL method. The EIS method is superior over the EL owing to its time saving and no incubation.
全 文 :!!"
" !
!
#
# $ % %
$
!
%
& ( )
&()*+(, &(-.,) &(*(,)
./01!"!*/1!
,234!# $ % %
ÈÜ|N±_jR;?a"õqz
!
3
5
E
%
5
FGH
%
5
3
5
I
#
5
JKE
6
5
LMN
!
"%1
ZgDE)&)F
5
Gw
$"%$$%$ #1
ZgDE)äÍ)F
5
Gw
$"%$$%$ 61
ÈÜ&()F
ß&MNlmþ
5
ÝÞÉß
>6$$$$$ !1
\X&()lmFß&MNí,yG&lmþ
5
gÖ
%$$$9%#
!
5
"
!5
6ÈÜÎEnÈÝ
#
â_1?
"
âÈÜ|N_jj$Méo¥
!
^ñ
)(&
7_jR
;{¼iw
!
yagi{¼7Êqz
&
_
ÎEnÈÝ
#
â_Ô_j?hÔ/0*+-
!#
â_?*+
!
ÎE_?hÔ/0ÈÝ_
!
t_ÎE_?R;ýÈÝ_
&
a
"õ%
B^)
^¤7
âB
"
!
!
"
!%!%
%
!%
D
!%
J
#
!%
%
!%
D
!
!
6
âB56:ï¬Þ?hÔ/0
!
¢%
%
!%
D
þ:?hÔ/0yagiÂ/à=÷H`C
!
?_B
%
%
!%
D
ñÈÜ|N?R;iw
&
a
"õ
")(
yagi?R;iw«&³h
!
ÐQ
%
-Ï/¹
!
û<®EÌX;
&
#$%
5
ÈÜ|N
$
a"õ
$
R;
$
%qÖa¡9ai
"%
%
#$
Ðýai
"%
D
#
&()*
! m9!81">555
+,-./
!,555
+01*
!%$$% ?"!>>"#$%%#$! ?$$#$ ?$"
¦§¨#
#$%$ ?$! ?%!$
©ª¨#
#$%% ?$6 ?$6&
«¬®
&fÑ;¼(lÒ
(
ÈÜfº£ãÓÔÕÑ[¬;G¹lm
) "#$$"$!$%7#
¿À
!¯SM¥ÄÅÆÇ
&
/..#..()5 ,&.%S-,0()#..&"!"#$%%&()%&N-%=,-2O&<=2-%(&).
7@ !2#$%,($-2T;<#0-)$#1<#$%,&.$&<@
APGEFkNE
%
5MGEFYJEQPDEF
%
5APGEFiGEF
#
5iN/AP/EFsNE
6
5AGEFINEFN/
!
"%1@$+&,&$:#$%&(%)! 3,%.*0+(0%1+42.5&%.()$:B&9&.5E1$C.2, $"%$$%$ #1@$+&,&$:B$%(.*0+(0%&! 3,%.*0+(0%1+42.5&%.()$:B&9&.
E1$C.2, $"%$$%$ 61?2(.(0(&$:#$%&(;*$+$,)! D.2J.12, 3*1C&A)$:#$%&(%)-*.&2*&54%0AQ.>6$$$$$
!1?2(.(0(&$:#$%&(;*$+$,)! ;25.%$2A&2(12C !%$(&*(.$2! @3#5E&.J.2, %$$$9%#
/7.%,-$%5G1+0.&5&%.JQGE J<2/3HGEHFD3<20GQ<3DQ/N3OD! GER UG0NGV0DT/3FDEDHJOJ<23/UD
KJESNGE /TKJEsJGEFLD3DNQDR4/0R PG3RJEDQQ/THPDQDDR0JEFQLGQRDHD3
HPGH/TKJESNGE 2/2N0GHJ/EQ4,!
!
"
!%!%
%
!%
D
GER %
J
# JE HPD&JEF0DB)J<2DRGEODQ2DOH3/QO/2S! LGQ/VHGJEDR VSHP3DD2G3G
!%
D
GER
!
# LGQGV0DH/VDNQDR HRDHD3
GER %
D
LGQDWH3D
GER %
D
O/N0R VDNQDR H/RDHD3
B)"%
%
# $ )WH3GOD0N0G33DQJQHGEOD"%
D
#
55
ÈÜ|N
"G1+0.&5&%.#
!·Ö×Ø
!
ùÓÃ?ú¿Ý
!
¡OXqÖÈÜ?ÙÚ
nÛ<êN
% $$$ b% "$$ <
?ê
!
ùXê$
FGF&?£ã`ÞÑ
"
&Üç
!#$$$#&
|N
û<R;>
%
a
%
Ý
%
²ç̹;±
!
¥O
XÑMnØÞ¹÷E?ß;>?à
n«?¿Ý
&
7ÈÜ|N±_j{¼R;?
qz
!
r_-._j?R*+
!
56©Må
5!
!
# µ
5
!ç
ÈÜ|N±_jR;?a"õqz
;<ÈÜ|N±_j?[¬;
!
56;<¢
^;
!
¥ÈÜ|NR¹¿Ý?ÄQnG¥
á1«x
!
¥¢G&
%
OPn()lm÷
3ÿ
&
ñ"R;iw?<
ag
"D0DOH3/0SHD0DG]GFD! )-#%
7»lâi
"LP/0D^
20GEHT3DD\D HDQHJEF! ZgC+#%
F Ú 9 ã t
" OP0/3/2PS0 T0N/3DQODEOD! C#%
* q %
"RJTD3DEHJG0HPD3
"D0DOH3JOG0J<2DRGEODQ2DOH3/QO/2S!)(&
^ña
"õ
")(
7Màá{¼lm
!
db
%9
¹
Ö#
!
ö¡"M)lmå?^ñk1-
îäe
!
Æ¥³iw"R;?
!
*$!¡
D
%
&näÍ-.?^ñê¡äE
"
憇
!
#$$8#&
Ð7É&"{¼
)(&
?lmæç
&
XÔ7
)(&
lmêèéÓ
!
ñ¢7ÈÜ
|NR;å?lm®:ÜgF³g
&
&lm
«ñag
")-#
na"õ
")(
âî7ÈÜ
|N{¼R;iw
!
qznÊü_jR;
?*+
!
ö7Êqz%
B^)
^¤7
â
)(&
B
"
êë !
%
êë?qÖà "
%
ai
%
n
%
%
%
Ðýai
%
D
%
ÐÔai
%
J
#
ìiR;?5
ó;
!
yagi{¼7Ê
!
t_
)(&
¡iw-
.ÈÜ|NR;?5¼;
!
RSiwR;?
&
%5
y
FEFG
xgyz
#$$"
$
%%
%\¾qr¡ÈÜÙÚêNÎE
íx3îÄw
!
â±_j
!
ÈÝ5ïð|N
vWG&~kNÄw
6
â±_j
!
«¬ê?W
ÚF
%&
Ñâ_j\è=Ä
#$
ȉj
!
ã÷»
½í
6$ b8$ MIê
!
Û_ñ
!
g(¤Ý
a,
&
¡â_jÔÄw?%»K«dj$ML
éo
%$ b#$
âò^
×\,ý$ó/B
&
m
FGU
{|}~O@OU
a
!
:-7RFGV,(5()&"%>#U !"#$%%&()%&<&<=2-%(&).
Hq
*/4
_j
g/2N0GJH/EQ
/0
-/EFJHNRD
r0
-GHJRNRD
,0HJHNRDr<
%
ÎE
i/EF0JN"i-%# ># !`7a8719p) !6 %`#a718p* % !%9
#
ÎE
i/EF0JN"i-## ># !`7a%6p) !6 %`!a61%p* % 6#"
6
ÎE
i/EF0JN"i-6# ># 8`%a6"1#p) !6 %`8a!!1!p* % 66>
!
ÎE
i/EF0JN"i-!# ># !`9a#6p) !6 %`8a#$19p* % %>9
8
ÈÝ
KJESNGE"KM%# >6 6`8a"1%p) !6 #`#a6>1%p* % 6"$
7
ÈÝ
KJESNGE"KM## >6 6`7a8#1!p) !6 #`#a6!1#p* % !!>
"
ÈÝ
KJESNGE"KM6# >6 6`8a8%1>p) !6 #`#a8%1>p* % 6#"
FEDG!T1
|}~E
a[ÄÒ4ÐôMa0*
!
a0*ÄÒ4
Ðô?<þ·õàán5ÄQ?öf;!GH
&
4Ð_û<-.a)f;
" &
&(1+4!%99##$
l÷n4Ðúû!
Ú
4ÐôÂjaø
&
4Сöâ,6Öâ,?
Y®8
!
ÚvMúônl÷ô<5óù"4Ð
7â , Q S T ? º q
" &HD2/E]NQ&(1+4! %99$$
-DV/3FED&(1+4!%99##!
?Y
!
56ña!lm"
j7â,1ù?A^
"i0D3N$$ /0D
j58a[
",#
ÄÒ"`ú
%
_S
!
ÄÒ
4Ðýûn4ÐÔ?ÊËü
,
`in`ú
f;
"i0D3N$#&
X`ú?Yf;nMf
;5ñçþa¡{¼
&
qziw-.
,
`i
1aÿ?
!
tܥ
)(&
"YGOREG0R!
%9>"$
憇
!#$$8#&
%1#1%5)(&
()*+*,-./01
5
ÈÜ|N
ÀÓ?
)(&
Í<³âý
!
ñ%
B^)
^¤
"
"
%#&
%
B^)
^¤¾þÖ
%$
âB
!
qr¥%qÖ
a¡9a
"3DQJQHGEODQJE HPDQJEF0D^B)=!=
%
#%
Ðýa
"DWH3GOD0N0G33DQJQHGEOD! =
D
#%
ÐÔ
a
" JEH3GOD0N0G33DQJQHGEOD! =
J
#%
ê ë
"3D0GWGHJ/E HJ
#%
ê ë ? q Ö à
"RJQH3JVNHJ/E O/DTJOJDEH/T
!
!
"
#%
%qÖa¡9a
i
"Q2DOJTJO3DQJQHGEODJE HPDQJEF0DB)! %!%
%
#%
Ð
ýai
"Q2DOJTJODWH3GOD0N0G33DQJQHGEOD! %
D
#%
ÐÔ
ai
"Q2DOJTJOJEH3GOD0N0G33DQJQHGEOD! %
J
#&
âB
?JnkÿBF¢
"ID2/&(1+4! %996!
%99!$ ISS22t&(1+4! %99>$
µ!ç
!#$$9#&
"
%5
gBâ?ÈÜ|N
%
$
Méoa"õ
CJF4%5(<2DRGEODQ2DOH3G/TE/E T^3/QH^
DW2/QDR /ED^SDG3^/0R QHD<
%1#1#5
2345678
5
Ñâ_j\Ä
#$
âj
$Méo
"
Ñââj
%
â
#!
ñö!`Ê¢ËÌ
6
î
!
å`q"Î
!
ÑâéoÒÞ
! O<
2Ó
%$
Ó
!
è=Ä
%
Ó
!#$
Ó¥
%
A
"
Ñââj
%
Ó
#!
Ñ
%#
& ( )
!"
"
5
â_j
%$
A
"%$
â/0
#!
qrñ°ò×ßÝ
Úlæ\{¼/
&
BâjÚ
!!
?8! ?%$! ?%8! ?#$! ?#8! ?6$! ?68! ?!$!
?!8 : %$
â/0¬0
!
¢! :
¥7
&
6
7 :,P
?%
ñiP/
!
÷=Ñâjw?/0
!
GH
#! P!
Ñâ_jï
%
AÕð
! :
B,~%â
#! P!
¢£
ù;{¼1â/0
&
%1#165
9:;<=
5
BFµ!ç
"#$$9#
?
&
%1#1!5
>?@ABCD
5
ÿ÷:? !
!
"
!%!%
%
!
%
D
!%
J
B
"M
#
#
y/0
"K
#
#
Æ"
"
"
##!
4y
/0?/à56~¥
-/FJQHJO&
$¬%
"0/FJQHJOQJFñ
&g&& %%18
!p
!
ÄÒ
-/FJQHJO
Pz÷ï$¬ï¤&S?/0
!
6YÆ
¥?/hÔ/0
!
©ÚìÿR;
&
)L
3
% MD
E,"@PH#
MR! "%#
_)
¥ !
!
"
!%!%
%
!%
D
!%
J
B\?³â
$H
¥/
/0
":#$ 3
¥
&
¤$¬#
oõ*¬?½í
$
E
¥i
$ @
¥&S/0
":#$ R
¥
&
¤$¬1õ
*¬÷
K
#?½í
&
"
#5
ÈÜ|N/-.Bâ/0
Ðýai
"%
&
#
?8
CJF4#5PGEFDQJE Q2DOJTJODWH3GOD0N0G33DQJQHGEOD"%
&
#
/TG1+0.&5&%.
FEHG
|}~E
ag i i w B
APGEF
ç
" #$$# #
n µ å
"#$$8#
&
%161%5
2345678
5
ÑââjÜj$Mé
o
%8 F!
ñö!`Öëí@`Ê¢ËÌ
6
î
!
"Î
ÒÞ
# O<
2Ó
!
ÊÑâ_jÔâj~
!
ܬ
8 F
¥
%
A
!
Ñâ_j
6$
A
" %$
â/0
!6
îR
`
#!
qrñ°ò×ßÝÚlæ&
Bâ
F
%1#1#&
%161#5
9EF<=
5
BFµ!ç
"#$$9#
?
&
%16165
>?@ABCD
5
ñ
&g&& %%18
!p
!
ÄÒ
kÿ
-/FJQHJO
P?&S/0~hÔ/0
!
ìÿR;
&
)
¥Â7agi
&
#5
yqz
DEFG
|}~EL
R@7(Y
7
"
âÈÜ|N_
%
$Méo¡-./0
1iw?aÿ÷ï?Bñ
&g&&
qz
!
Ï
-/FJQHJO
P{¼
&
¤$¬z
!
kÿï
hÔ/0Ö¢8k1
!
7zæ0{¼qz
&
_j
%
D
ªè/08/0-
&
¤$¬
!
6
-/FJQHJO
P¥^¤
!
/Ò¬;ªÛqz
!
56z
ïâ_j?hÔ/0
!
3
#
5:
_j
hÔ/08¯0¥
?#91> b?6%1" :!KM#
Q
ý
" ?#91> :#!i-!
Q
" ?6%1" :#&
ÎE_j
*+-
!
H*¥
?$16 :$
ÈÝ_j?*+
-
!
H*¥
?$17 :!
ÐùÎE_j?
-+
8$
2ÈÝ_j
!
¤ïÎE_j?R;ýÈÝ
_j?/0
&
Â/à8k1¡
$1>7$ b$19%"!
=÷
$1$%
`C
!
(E[=÷Z
$1$$%
555
m
DG
)
#
&)
F
&
!
!
:-7RDG:>#.#;(2#%>-2%#;<#,-%=,##.%(;-%#07@ %>#<-,-;#%#,)
#
&)
F
-)0
!
_j
g/2N0GJH/EQ
hÔ/0
&D
8k1
IGEFD
Qý/0
YGWJ
Q/0
YJEJ
üwà
=
#
/DTJOJDEH/T
RDHD3
=
/33D0GHJ/E
O/DTJODEH
%
D
%
%
!
%
D
%
%
!
%
D
%
%
!
%
D
%
%
!
%
D
%
%
!
i-% ?6%18 ?6%1# ?6%1$ ?6$1$ ?#91> ?#918 ?661% ?6#17 ?6#18 $1"># $1>%# $177%
$1>>!
!!!
$19$%
!!!
$1>%6
!!
i-# ?6%1! ?6%1% ?6$18 ?6$1! ?6$1% ?#91# ?6#18 ?6#1$ ?6%1> $1>66 $1>77 $1"8!
$19%6
!!!
$196%
!!!
$1>7>
!!
i-6 ?6%1! ?6%1" ?6$16 ?6$1# ?6$17 ?#91% ?6#17 ?6#1" ?6%18 $1"69 $1"96 $1"$6
$1>7$
!!
$1>9%
!!!
$1>6>
!!
i-! ?6%1" ?6%17 ?6%17 ?6$18 ?6$18 ?6$16 ?6#1> ?6#1" ?6#19 $1>!$ $1>6> $17>#
$19%"
!!!
$19%8
!!!
$1>#7
!!
KM% ?6$16 ?6$1> ?6$1! ?#91$ ?#919 ?#918 ?6%17 ?6%1> ?6%16 $1"96 $1>!> $1"!>
$1>9%
!!!
$19#%
!!!
$1>78
!!
KM# ?#91> ?6$1% ?6$1# ?#>17 ?#>19 ?#91% ?6%1% ?6%1# ?6%16 $1""7 $1>%$ $1"8%
$1>>%
!!!
$19$$
!!!
$1>7"
!!
KM6 ?6$1! ?6$1% ?#>16 ?#>1> ?#>1> ?#71" ?6%19 ?6%16 ?6$1$ $1>#" $1>87 $1"7"
$19$9
!!!
$19#8
!!!
$1>"7
!!!
55
! !
!;$1$8$
!!
!;$1$%$
!!!
!;$1$$%!
1.
& +PDQG
5!
!
# µ
5
!ç
ÈÜ|N±_jR;?a"õqz
`C
&
ÄÒzP
!
5kÿïhÔ/0n
98_
Ú¼N
!
~
â_j?hÔ/0
98_
Ú¼N<-.P0?R
&
?_â_j9
?hÔ/0*+-
&
3
#
54
_j
%
%
ªè/08óñ
-/FJQHJO
P{¼üÝz
!
_jhÔ/08
¯0¥
?6$1% b ?6%1" :!KM#!KM6
Qý
"
¥
?
6$1% :#!i-6
Q
" ?6%1" :#&
ÎE_j*+
-
!
H*¥
?$17 :$
ÈÝ_j?*+-
!
H*¥
?$1" :!
y
%
D
Âõ
!
ÎE_j?
-+
8$
2ÈÝ_j
!
-¤ïÎE_j?R;ý
ÈÝ_j?/0
&
Â/à8k1¡
$1>9% b
$196%!
=÷
$1$$%
`C
&
ÄÒzP
!
5
kÿïhÔ/0n
98_
Ú¼N
!
~
â_j?hÔ/0
98_
Ú¼N<-.P0
?R
&
?_â_j9?hÔ/0
"-+
8$
#
*
+-
&
3Y54
_j !ªè/08óñ
-/FJQHJO
P{¼üÝz
"
##!
_jhÔ/
08¯0¥
?#>16 b ?6%17!KM6
Qý
"
¥
?
#>16 :#!i-!
Q
" ?6%17 :#& KM6
?hÔ/
0
"-+
8$
#
_ý¢£_j
!KM6
?hÔ/0
98_
Ú¼N¥
?#71" b6$1$ :!
y
i-!
?hÔ
/0
98_
Ú¼N
" ?6$16 b?6#19 :#
ÛR
!
y¢£_j<-.P0?R
!
__j
KM6
?
hÔ/0ý_j
i-!!
Úy¢£_j<<
?*+
&
ÎE_j?hÔ/0ª¥
?6$19
:!
ÈÝ_j
-+
8$
ª
" ?#917 :#&
Â/à
8k1¡
$1>%6 b$1>"7!
=÷
$1$%
`C
!
_j
KM6
=÷
$1$$%
`C
&
5ÿ
7
âB
"
!
!
"
!%!%
%
!%
D
!%
J
#
kÿï?
hÔ/0{¼îÊü
!
B
%
D
!%
%
!
!¡-.
?/01¢qÖÞ
&
¤$¬
!
/
-/FJQHJO
Pz
5:÷hÔ/0
!
ö
98_
?Ú¼Nü2
$
B
%!%
J
!
"y/0?/à<<~ï_?
&
$¬
!
/
-/FJQHJO
Pzg:÷hÔ/0
!
?
_B
%!%
J
!
"-ñÈÜ|N_?R;
iw
&
DEDG
|}~E(Y
ñÂ7agiy/0?7^/à
!
ÄÒkÿ
P?&S/0~`úhÔ/0ª
!
ìÿR
;
&
¢hÔ/0Öüwà
%
Â/àF
6&
_jÂ7agiªè/08óñ
-/FJQHJO
P{
¼üÝz
!
_jhÔ/08¯0¥
?6%17 b
681% :!KM#!KM6
Qý
-+
8$
.¥
?6%17 :!i-!
Q
" ?681% :#&
_j
i-!
?hÔ/0
98_
Ú¼N
¥
?6!1" b?6818 :!
y¢£_j?hÔ/0
98_
Ú¼NÛR
!
__j
i-!
?hÔ/0
¢£_j
&
©hÔ/0
98_
Ú¼N<Û
RK î54
i-6
?
-+
8$
i-%!KM#!
KM6!i-#
KM#&
ÎE_j?hÔ/0
ª¥
?6619 :!
ÈÝ_j
-+
8$
ª
" ?6%1" :#&
Â/à8¡
$1977 b$1996!
=÷
$1$$%
`
C
&
3ag:ï?hÔ/0óy
-/FJQHJO
P
=÷Âjý?z0
!
þ6³)?6agi
¥ÛÜ
!
!Ç*¢£?Ì0
&
m
HG
@rR
Z#R
:-7RHG:>#.#;(2#%>-2%#;<#,-%=,#& $""($(#)%&"0#%#,;()-%(&)-)0$&,,#2-%(&)$""($(#)%$-2$=2-%#0
7@ #2#$%,&2@%#2#-W-5#"!?#
_j
g/2N0GJH/EQ
hÔ/0
&D
8k1
IGEFD
Qý/0
YGWJ
YJEJ
=
#
/DTJOJDEH/T
RDHD3
Â/à
=
/33D0GHJ/E
O/DTJODEH=
i-% ?6#19 ?6#16 ?6618 $199%
$1997
!!!
i-# ?6617 ?6#1" ?6!18 $19"7
$19>>
!!!
i-6 ?6!1$ ?6618 ?6!18 $199%
$1997
!!!
i-! ?681% ?6!1" ?6818 $1996
$199"
!!!
KM% ?6#1$ ?#919 ?6!1$ $1977
$19>6
!!!
KM# ?6%17 ?6$1" ?6#17 $19>"
$199!
!!!
KM6 ?6%17 ?6$18 ?6#1" $19>6
$199%
!!!
DEHG
\@7
ñagi:÷?hÔ/0yaB
:÷?hÔ/0{¼7Êqz
!
F
!&
6ag
i:÷?hÔ/0¥ÛÜ
!
ñ
Bg&61$%
iwï
:ï?hÔ/0?Â/à
!
¢y6
B
%
%
z:÷?hÔ/0?Â/àQE
!
¥
$19$!$
Úy6B
%
D
z:÷?hÔ/0?Â/à
î9
!
¥
$1>>!$
y6B !z:÷?hÔ/0
?Â/àQ2
!
¥
$17>9&
¡a!
aB
%
%
!%
D
ñÈÜ|N_R;?iw
&
6#
& ( )
!"
"
5
m
IG
bZ#LR@@Z#(Y
:-7RIG:>#$&,,#2-%(&)-)-2@.(.&"%>#.#;(2#%>-2%#;<#,-%=,##.%(;-%#07@ !?-)0%>#<-,-;#%#,.&"!T1
_j
g/2N0GJH/EQ
agiz?
hÔ/0
&D
B
%
D
z?
hÔ/0
&D
D
B
%
%
z?
hÔ/0
&D
%
B !z?
hÔ/0
&D
!
i-% ?6#19 ?6%18 ?6%1# ?6%1$
i-# ?6617 ?6%1! ?6%1% ?6$18
i-6 ?6!1$ ?6%1! ?6%1" ?6$16
i-! ?681% ?6%1" ?6%17 ?6%17
KM% ?6#1$ ?6$16 ?6$1> ?6$1!
KM# ?6%17 ?#91> ?6$1% ?6$1#
KM6 ?6%17 ?6$1! ?6$1% ?#>16
Â/à
/33D0GHJ/E O/DTJODEH %
$1>>!
!!
$19$!
!!
$17>9
DEIG
|}~E(Y
Äñagi
!%
D
!%
%
6
âBkÿï?âÈÜ
|N_j?hÔ/0{¼?9ÛR`+á
kqz
!
3
8
54
_j
%
RB*+=
÷ZH`C
!
{Ú7ÈÜ|N_j?h
Ô/0
"-+
8$
#
{¼[RÊü
!
F
7&
ÈÝ_
j
"KM#!KM6!KM% #
?hÔ/0<<*
+
!
ÎE_j
"i-%!i-#!i-6!i-!#
g=÷
*+?`C
!
ÐùÈÝyÎE
#
âê.?hÔ
/0=÷H*+`C
!
ÈÝ_jHýÎ
E_j
!
©Ú?_ÎEêNÈÜ|N?R;
ýÈÝêN
&
m
MG
|}~(Ym
:-7RMG/)-2@.(.&"3-,(-)$#%-72#7-.#0&)?:
MX
()<&<=2-%(&).&"!*%&()%&
8+!Ý
.G3JGHJ/E Q/N3OD
Cn
&&
°30
BC
G-
C
ª
CUG0ND
`C
&JFEJTJOGEH
0DUD0
_j
,%61>"9 7 #16%6 # %$1!$9 $1$$$ !
B
gG3G
+*
)3/3 #1777 " %# $1### #
8+
+/HG0UG3JGHJ/E
681>>8 " #$
m
YG
|}~W ¡¢
:-7RYGA=2%(<2#$&;<-,(.&).7-.#0&)?:
MX
&"<&<=2-%(&).()!*%&()%&
_j
g/2N0GHJ/EQ
Cª
YDGE
ÛÜ*
&HGERG3R
RDUJGHJ/E
8_
`C
8_ QJFEJTJOGEH
0DUD0
%_
H`C
%_ QJFEJTJOGEH
0DUD0
KM# ?6$18 $197! ! G ,
KM6 ?6$1" $1"96 " G ,@
KM% ?6%1$ $1>"6 " GV ,@
i-% ?6%19 $19$" ! VO @B
i-# ?6#1$ %1678 $ OR B
i-6 ?6#1! %1!## ! OR B
i-! ?6#1> %199# 8 R B
55
ÈÜ|N-.RB?[RÊüv
"!
©\56#ï
%
D
!%
%
#
âB:÷?hÔ/
0<<*+
!
Ð.Hýagiþ:?h
Ô/0
&
©Ú?_3ag:ï?R;ãýa
"õ
&
m
UG
|}~LR ¡¢
:-7RUGA=2%(<2#$&;<-,(.&).7-.#0&)?:
MX
&"
,#.(.%-)$#<-,-;#%#,.()!*%&()%&
B
gG3G
YDGE
ÛÜ*
&HGERG3R
RDUJGHJ/E
8_
`C
8_ QJFEJTJOGEH
0DUD0
%_
H`C
%_ QJFEJTJOGEH
0DUD0
%
D
?6$19 $1"!6 # G ,
%
%
?6$19 $178$ 6 G ,
agi
/ERNOHJUJHS
?661$ %1668 % V @
"
65"
âÈÜ|N_j«hÔ/0?i"
CJF4650NQHD3F3G2P VGQDR /E -+
8$
/T" 2/2N0GHJ/EQJE G".&5&%.
DEMG
|}~E[)(Y
5ÿ-.Riwkÿï?_j?
h Ô / 0
!
«ñ 3, ÿpC i
"jgiY,#
7
"
âÈÜ|N_j{¼iqz
!
©
ѱ"56#ï
!
ÎE_jyÈÝ_jq¥_?
#
!
©Ú?_âêS?_j¡R;K¥¡
³w?*+
"
"
6#&
!#
5!
!
# µ
5
!ç
ÈÜ|N±_jR;?a"õqz
65
BA
HEFG
|}~E
R;ù"¡7/RBí,?I#^
!
ÄÒ&?ØÞ8+n°±ÄQ-:?³
RóF
"i0D3N8#!
ù"7/I#^?
³ØÞf;
&
Ð
ØÞf;Í<¡fwí,o
p.g1
!
æó=ï!
!
èí,?®85yê
"
¯SMç
!#$$>#&
&lmâî7qÖÈÜÙÚ
êN?ÈÜ|N±_j{¼ZR;lm
!
_
ÎE_?
!
â_jR;<<*
+
!
ÈÝ_?
6
â_j<<¯Þ?*+
!
ÐùÎE_R;ãýÈÝ_
!
qz¢v?
5óù3jêfgºÞ?
&
Ý!/
"%9>9#
n&Üç
"#$$$#
lm¤¥
!
ÈÜÙÚZ0ê<
ß/¨?¥¡
&
ÿÙÚ1Ê°
%97%*%979
$r
Ũi[!
ß/>0Q>?
%
%AC>0¥
918 :!
QE
##17 :!
ß/¨
%
%C©0¥
98$ QE¥
# $"" <&
©êå*i¿
56#ïY
ß/Q>ù¡
>$$ b% 8$$ <
9?:
!
êù|&d\qÖ
!
¢
%
%C/n
CQ/qr¥
?718 b?71" :
n
?%$1! b
?%618 :!
ü:?Kºn1ºqr÷ý
%17 b
"18 :
n
!1# b>16 :& %
%Aß/?C2.iÑ
%$$ <
¥
#1% :!
QE=
"16 :&
% 8$$ <
6K
ß/>0ôõ-
!
|&qÖôõ-5
&
Y
ß/³)©
%%
%%
1¾db¯Þ
!
֔$
6
%K
¾y&
ÈÝ_j?C¥
% 6># <
ÎÏ
!
ÚÎ
E_j?C¥
% 6%> <
ÎÏ
!
3êÂ*
"$ <
ÎÏ
!
©ghÈÝ«¬ê?C/ãýÎE¬
ê
%18 :
ÎÏ
!
|N_j/ÒI#?í,^
!
©
Úì_j?R;M*+
!
ìÈÝ_j
"
hÔ
/0
?6$1" :#
?R;ÎE_j
"
hÔ
/0
?6#16 :#&
¡_j!i-!
?Q
"% %>9 <#!
ÐhÔ/0Q?56ñß/
¨?¥¡%W
& #$$"
$
%%
%1¾
!
¡*ꫬÒ
P(¤ÈÝ_j\¥¡¸gò£?âj
!
©Ú;
<﫬ê?/üý
&
HEDG
E
¡7µ4¨
"@1+.(&A$2# !
ân5v
"K%&5.+&1##
â"?R;iwlm\(¤
!#
þ÷ï?R;Âõ
"YGEONQ/&(1+4!
#$$!#&
¡6W7|
"!.20)+5&(%.#
R;lm(¤
R;ü
!)(&
þ÷ï?R;y
)-
Â*
$
jR;m>
!)(&
<5óÊ
)-
ìR;
"ID2/&(1+4! %99!$ ISS22u &(1+4!
%99>#&
ñ
)(&
%
D
B÷ï?6W7|À?R
;
)-
÷ï?ûFR;
"ID2/&(1+4! #$$$$
cN3
)(&
:÷?
R;
)-
!
y:?lm«&Â.
!
ûjv?<8{³|lm
&
¡76W7|`Ñj
$Méo¥#
%
$?+\(¤
)(&
%
D
B÷
ï?ÀR;y
)-
w?ûFR;9¥¡
m>?Â/;
"%h$19"#"ID2/&(1+4! %998$ %99"#&
&lm!)(&
ñ
%
%
!%
D
ìk?hÔ/0y
)-
A*
!
Â/àqr¥
$19$!!$1>>!!
=÷
$1$%
`C?HÂ/
!
y:lm?Âõ
&
¡
)(&
?
7
âB!%
%
!%
D
zQÝ
!
qz¢v?5óùy"
¡/1ù1
!
4Ð
ôôõö÷9,
!
4Ðô;ôõ.E
!
ÐÔa%ú
ô|ýö
!
©ÚºÞÐý?ai
%
D
ô|-2
!
y
)-
?iwv«&Â.
!
Íùiw-.
&
3
f_
=
D
h=f=
%
"
µ!ç
!#$$9#
5:
%
D
h%f%
%
!%
%
y/0-¤
&
¤$¬8/0
!
Ú
%
«&K-
ö/0?@AGH:w
!
©Úì:
%
D
y/0
-¤ï
&
¤$¬8/0
!
?Y
!
ù
%
%
?8
ghÐýai
%
D
?8
&
%qÖa¡9a
=
%
¡ÀÓûj(?`ú
%
_S<8{³|
lm
&
)-
56:裡
%
æ
%
?ÿ
!
ö:
÷?hÔ/0Êüæ
!
z$¬A*
&
¤$
¬
!
?Y³)6agi:÷?hÔ/0Æ¥
ÛÜ
!
!Ç*¢£?Ì0
&
3
!
54
ag
i:÷?hÔ/0y
)(&
%
%
!%
D
Bz:
÷?
-+
8$
Â/àý
!
?_ññÈÜ
|NR;?lm
&
ñ
)(&
iw"?R;
-Ïã{¼;?)iw
!
-Ïã7"¬&
{¼/¹
!
GH4Ð?°±±N
!
üÝ?G&Z
$
Yfr¬&E?_jR;l
m
&
Ú
)-
³´
!
ì
êë°±±N
!
ù¢£ãS
& )(&
X
%
fñêiw"?
R;
!
ù³lm"R;<þ?
!
¡
R;iw
!
ý¢ù_jRlmKû<¹Ý?þ
&
L M + ,
&Ü
!
<=±
4#$$$4
|&¿Ý
?
ÙÚ|&îlm
4
g
Ö
\X&ï´×
4
Ý!/
4%9>94
ÈÜ|>?MNfg?lm
4
MN)ÚV
!>"!#
#! ?!"4
¯SM
!
Á@A
4#$$>4
¯Ñß;lm
4
8
8()µpï´
×
!6$ ?6%4
8#
& ( )
!"
"
5
µ
5
å
4#$$84
XýÉ&"R;iwî¤
4
¹º&lm
!
%>"8# %! ?#$4
µ
5
å
!
BVC
!
æÓÀ
4#$$84
iw"R;a"?
4
"
Myq@M)³
!6%"%# %9 ?#74
µ
5
!
!
½DE
!
µ
5
å
!
ç
4#$$94
a"õ¡Fêú¿Ý
R;iw\?^ñ
4
"ØÞ¿Ý)³
!%$"6# !%9 ?!#84
/0D
QP//HQ4kc/3HJO&OJ!7!"6# #!9 ?#8"4
i0D3N< 4%9>$4)0DOH3JOG0J<2DRGEOD HDOPEJoNDQJE 2PSQJ/0/FJOG0
QHNRJDQ4*DLADG0GER k/N3EG0/TC/3DQH3S&OJDEOD! %$ " % # %97
?#$"4
i0D3N<4%9>84C3/QHPG3RJEDQQ/TO/EJTD3/NQQDDR0JEF 23JEOJ20DQGER
G220JOGHJ/EQrrBN3SDGY-4)UG0NGHJEFQDDRJEFoNG0JHS 23JEOJ20DQ!
23/ODRN3DQ!GER 23DRJOJHJUDGVJ0JHJDQ/T
cN3
IDQ!#" "%7 ?"#64
-DV/3FED*! BN2/N ^v\GEED-! +DN0Jw3DQ! &(1+4%99#4-GHD3G0GER
3/HGHJ/EG0;0*1+)<(0,022.ON0HJUG3QDWPJVJHJEFRJTD3DEHT3DD\JEFH/0D3GEOD4
g0GEHgPSQJ/0!%$$ #!7 ?#8!4
YGOREG0R kI4%9>"4(<2DRGEOD&2DOH3/QO/2S4*DLM/3] k/PE ZJ0DSGER
&/EQ!(EO4
YGEONQ/&!*JODQDCg!YGQJ)!&(1+4#$$!4/<2G3JEFT3GOHG0GEG0SQJQ!
D0DOH3JOG0J<2DRGEODGER D0DOH3/0SHD0DG]GFDT/3HPDGQQDQQ
@J/HDOP!"9"!# 7#" ?76#4
ID2/+4%99!4(ET0NDEOD/TRJTD3DEHD0DOH3/RDQGER HJQQNDQ/E HPD
J<2DRGEODQ2DOH3G/T&O/HQ2JEDQP//HQ4)0DOH3/
QP//HQ/T&O/HQ2JED JEH3GOD0N0G33DQJQHGEODO/33D0GHDQLJHP T3/QH
PG3RJEDQQ4rrg3/ODDRJEFQ/THPD CJEEJQP k^G2GEDQD Z/3]QP/2 /E
Y/0DON0G3GER gPSQJ/0/FOJG0,Q2DOHQ/T/0R GER PJ0JEF+/0D3GEOD
/T*/3HPD3E 3/2Q4k/]J/JEDE!CJE0GER!#" ?6$4
ID2/+!-DJE/EDE Y!gxx]]tEDE +4%9984+PDG220JOGHJ/E /TD0DOH3JOG0
J<2DRGEODQ2DOH3/QO/2SJE GQQDQQJEFHPDT3/QHPG3RJEDQQ/T&O/HQ
2JED4g3/ODDRJEFQ/THPD(K4(EHD3EGHJ/EG0/ETD3DEOD/E )0DOH3JOG0
@J/^J<2DRGEOD4cDJRD0VD3FriD3
Q2DOH3/QO/2S/T&O/HQ2JED"!.20)+5&(%.-4# QP//HQJE 3D0GHJ/E H/
O/0R GOO0J
D0DOH3JOG0OJ3ONJH4k/N3EG0/T)W2D3J
ID2/+!APGEFY!ISS22t,!&(1+4%99!4)TDOHQ/TT3DD\D^HPGLJEsN3S/E
2G3G
ISS22t,!ID2/+!.G2GGUN/3J)4%99>4BDUD0/2
HD<2D3GHN3DQ4GE kC/3IDQ!#>"!# 88" ?8784
&
%#$ ?%#94
&HD2/E]NQg-!-SEOP B.!jD
APGEFi!ISS22t,!ID2/+4#$$#4+PDD0DOH3JOG0J<2DRGEODQ2DOH3/QO/2S
/T &O/HQ 2JED EDDR0DQ RN3JEF O/0R GOO0J
APGEFY!ZJ0JQ/E kcY4%99#4)0DOH3JOG0J<2DRGEODGEG0SQJQJE 20GEH
HJQQNDQ HPDDTDOH/TT3DD\D^HPGLJEsN3S/E HPDD0DOH3JOG023/2D3HJDQ/T
2/HGH/HNVD3GER OG33/H3//HHJQQNDQ4GE kg0GEH&OJ!"# 8!8 ?8864
!
!"#$
5
GHI
"
7#