Fraction 8 of the extractants from semen pharbitidis was identified and its mechanism of biological activity against Tetranychus cinnabarinus was investigated. The main component of the extractant fraction 8 was detected with nuclear magnetic resonance (NMR), gas chromatography and mass spectrum (GC-MS). Influences of the main component on several enzymes activities such as glutathione-S-transferase (GSTs), acetylcholine esterase (AChE) and mono-amine oxidase (MAO), in T. cinnabarinus body were detected by using biochemical method. Results showed that the main component of the extractant fraction 8 was methyl linoleate. The methyl linoleate activated glutathione-S-transferase in T. cinnabarinus body, while it inhibited to various degrees acetylcholine esterase and mono-amine oxidase related to nervous system, and hence caused the death of T. cinnabarinus. Therefore the methyl linoleate has a good prospect developing into new pesticide.
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