Abstract:This paper dealt with the biological rhythm of sex pheromone of the females and the responsive-ness of the males of Illiberis pruni.The methods of electroantennogram and wind tunnel assay were used.The results showed that seX pheromone production occured in the newly emerged and 1 to 7-day-old female moths,but the maximum level occured in 3-day-old female moths,while the 3-day-old male moths had the maximum responsiveness tO seX pheromone,There were two peaks of both the sex pheromone production of the females and the responsiveness of the males in a day;the male response peaks lasted longer.This indicated the sexual sensitivity of the males and the harmonious synchronization of the sexual behaviour between the females and the males.