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作 者 ：李连昌 樊永亮 任自立

期 刊 ：林业科学 1991年 27卷 2期 页码：145-148

关键词：枣粘虫；性信息素；时辰节律；性信息素腺体；腺体提取；瓶内收集；触角电位；

Keywords:Ancylis sativa, Circadian rhythm, Sex pheromone gland, Sex pheromone, Extraction of sex pheromone glands, Vial washing collection, Electroantennogram (EAG),

摘 要 ：本文利用腺体提取、瓶内收集、触角电位和田间试验等方法对枣粘虫产生和释放性信息素时辰节律进行了研究。其中瓶内收集性信息素的方法,在研究昆虫释放性信息素时辰节律方面报道很少,更重要的是,此法在我们的实验中取得了比较满意的效果。研究结果表明:(1)羽化后1—6天的提取物产生的触角电位无明显差异,说明性信息素在此期间的产生与日龄无关(2)腺体中性信息素的含量高峰在凌晨4点,而从体内往体外释放的高峰在早晨7点。(3)利用处女雌蛾不同时辰的诱蛾量的田间试验也进一步证明了枣粘虫产生和释放性信息素与交配行为在时辰节律上的同步性。

Abstract:This study deals with the circadian rhythms of sex pheromone production and release in the jujube leafroller moth Ancylis sativa Liu. The methods of extraction of sex pheromone glands (SPG) , vial washing collection and electroantennogram assay were used. The results showed that the sex pheromone production of the female moths were not significantly different in the first six days after adult emergence, and the peak of the content of sex pheromone in the glands was seen around 04:00 hours and the peak time of sex pheromone release was around 07:00 hours. The observation of male moth attracted by virgin female in the field indicated a good coincidence with the sex pheromone production and release in the laboratory.

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