Abstract:This paper is studied the inoculation for development of ectomycorrhizae on container-grown tree seedlings of some commercially important Chinese tree species with two different types of inocula of the ectomycorrhizal fungus Pisolithus tinotorius (Pt). Results show that the inoculation of these tree species tested is successful with solid-base cultures of mycelium of this fungus. Excellent Pt, ectomycorrhizal root de velopment (Pt index 70+ and Pt ectomycorrhizal percentage 95%) is produced on the seedlings of the four pines tested. The typical (large, swollen, golden, bifurcate) Pt ectomycorrhizae observed on these four Chinese species are identical to those observed on Loblolly Pine. It confirms that like Loblolly Pine these pine species are hosts of Pt fungus and implys that there is a great potential to use the techniques for development of ectomycorrhizae on these species in forest production. However, spore pellet inoculum is considerably less effective (Pt index 10 and Pt ectomycorrhizal percentage<20%). No ectomycorrhizae on Chinese Fir, Chinese Arborvitae and Siberian Eim are observed in this experiment. This suggests that these species most likely are endomycorrhizal or/and facultative ectomycorrhizal tree spectes. Besides, this study also provides techniques and methods for development and evaluation of ectomycorrhizae on container-grown tree seedlings for forest research and production in China.