Abstract:Callus derived from completed embryos of Ginkgo biloba L. were light green and crisp. And after subculture, some callus changed from crispy to compact. In MS medium supplemented with 2mg/L BA and 0.5mg/L NAA, somatic embryos were obtained from the compact callus. Embryoids originated from embryonic cell at the edge of callus, and embryoids at whatever stage (globular or heart) exhibited a slow growth, which delayed transfer onto subculture medium.