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作 者 ：刘翠琼;汤浩茹;罗　娅

期 刊 ：园艺学报 2005年 32卷 6期 页码：1080-1083

关键词：梨；叶片；再生；组织培养；

Keywords:Pear, Leaf, Regeneration, Tissue culture,

摘 要 ：以‘巴梨’、‘身不知’和‘早酥’梨试管苗叶片为外植体, 对不定芽进行了诱导、增殖和生
根。重点探讨了基本培养基、植物生长调节剂配比、AgNO₃ 不同浓度和NH4⁺-N与NO3^--N比例对不定芽再生的影响。结果表明, MS + TDZ 0.5 mg/L + IBA 0.1 mg/L为‘巴梨’叶片不定芽发生的最佳培养基。在QL + TDZ 1.0 mg/L +NAA 0.1 mg/L培养基上, 暗培养3周后转光下培养, ‘身不知’和‘早酥’分别获得89.6%、81.2%的不定芽再生率和3.45、3.73的平均再生芽数; 对‘巴梨’和‘早酥’不定芽再生有效促进的AgNO₃ 浓度范围为0.1～0.5 mg/L, 0.1～4.0 mg/L的AgNO₃、1∶2～7的NH₄⁺-N∶NO ₃^--N和21.50mmol/L的K+对‘身不知’叶片再生均有促进作用, 缺乏NH₄⁺-N不利于不定芽的再生; 转移到MS+BA1.0 mg/L + IBA 0.1 mg/L培养基上能快速增殖; 在MS、1 /4MS + IBA 1.0～2.5 mg/L +蔗糖5～15 g/L +活性炭0.5～1.0 g/L上获得了不同程度的生根苗。

Abstract:Taking leaves from the vitro cuttings of Pyrus communis L. ‘Bartlett’, P. pyrifolia Nak.‘Shenbuzhi’and P. bretschneideri Rhed. ‘Zaosu’as explants, the adventitious bud were induced, propagated, and rooted. The effects of basic media, plant growth regulators and their concentration, AgNO₃ with
different concentration and the ratios of the nitrate: ammonium on adventitious bud regeneration were studied.The results showed that the best bud regeneration medium from leaves of‘Bartlett’wasMS + TDZ 0.5 mg/L+ IBA 0.1 mg/L. The bud regeneration rate of ‘Shenbuzhi’ and ‘Zaosu’ respectively from leaves was reached 89.6% and 81.2% , with an average regeneration buds of 3.45 and 3.73 on QL + TDZ 1.0 mg/L +NAA 0.1 mg/L after three weeks dark pretreatment. The bud regeneration frequency of‘Shenbuzhi’from
leaves was enhanced on medium containing AgNO₃ 0.1 - 4.0 mg/L, 1∶2 - 7 of the nitrate: ammonium with K+ 21.50 mmol/L, while inhibited without nitrate; The subculture medium for in vitro proliferation of three cultivars was MS +BA 1.0 mg/L + IBA 0.1 mg/L; The rooting rate in varying degrees was obtained in the media of MS, 1 /4MS supplemented with IBA at 1.0 - 2.5 mg/L and sucrose at 5 - 15 g/L and AC ( actived charcoal) at 0.5 - 1.0 g/L.

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