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作 者 ：曹建康;姜微波

期 刊 ：园艺学报 2005年 32卷 5期 页码：783-787

关键词：Acibenzolar-S-methyl；梨；鸭梨；链格孢菌；抗病性；

Keywords:Acibenzolar-S-methyl, Pear, Yali pear, Alternaria alternata, Disease resistance,

摘 要 ：Acibenzolar-S-methyl (ASM) 是重要的植物抗病基因诱导剂。鸭梨果实经过负压渗透ASM诱
导处理后接种黑霉病(Alternaria alternata) 。结果表明, 0.5 mmol·L ^{- 1} ASM能有效抑制鸭梨果实损伤接种病害和自然侵染病害的发生。ASM处理果实具有较高的苯丙氨酸解氨酶( PAL) 和几丁质酶(CHT) 活性, 积累了较高水平的H₂O₂ 和酚类物质。离体试验表明, ASM不对病原菌生长产生抑制作用。ASM处理对果实病害的抑制作用可能与其增强了果实抗性系统有关。

Abstract:Acibenzolar-S-methyl (ASM) is an important inducer of genes related to disease resistance in plant. ‘Yali’pear fruit were wounded and inoculated with Alternaria alternata after vacuum infiltration of ASM. Results showed that 0.5 mmol·L ^{- 1} ASM treatment effectively inhibited the development of black mould disease in inoculated fruit or in intact fruit, enhanced activities of phenylalanine ammonia-lyase ( PAL) and chitinase (CHT) , and led to accumulation of H₂O₂ and total phenolic compounds. In vitro experiments indicated thatASM did not inhibit the growth of the fungus, imp licated that the alleviation of fruit disease by ASM treatment is attributed to its ability to enhance defense system in fruit rather than its direct inhibitory effect on the fungus.

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