全 文 :!23" !6#
Vol.23 No.6
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ACTA AGRESTIA SINICA
2015$ 11%
Nov. 2015
犱狅犻:10.11733/j.issn.10070435.2015.06.011
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犐犿狆犪犮狋狅犳犆狔狋狅犽犻狀犻狀狅狀狋犺犲犃狀狋犻狅狓犻犱犪狋犻狏犲犘犺狔狊犻狅犾狅犵狔狅犳
犆狉犲犲狆犻狀犵犅犲狀狋犵狉犪狊狊狌狀犱犲狉犇狉狅狌犵犺狋犛狋狉犲狊狊
LIUYang1,LIRongquan2,ZHANGXunzhong3,CHANGZhihui1
(1.TurfInstituteofBeijingForestryUniversity,Beijing100083,China;2.ShenyangLanxiGreenTechnology
DevelopmentCo.,Ltd.,Shenyang,LiaoningProvince110000,China;3.DepartmentofCropandSoilEnvironmental
Sciences,VirginiaPolytechnicInstituteandStateUniversity,Blacksburg,VA24061,USA)
犃犫狊狋狉犪犮狋:Inthisexperiment,theeffectofcytokininonCreepingbentgrass(犃犵狉狅狊狋犻狊狊狋狅犾狅狀犻犳犲狉犪)oxidize
resistantphysiologybysprayingdifferentconcentrationsofexogenouscytokininwasstudied.Treatments
includedtwomoistureconditions:welwateredanddroughtstress;and3concentrationsofcytokinin:0,
10and100μM.Leafrelativewatercontents,levelsofreactiveoxygenspecies(O
-
2 andH2O2),superoxide
dismutase(SOD),catalase(CAT),peroxidase(POD)andascorbateperxidase(APX)activities,andma
londialdehyde(MDA)contentsweredetermined.Theresultsindicatedthatcytokininreducedthegenera
tionrateofO-2 andH2O2,MDAcontentsofcreepingbentgrasscomparedtocontrolunderbothwaterre
gimes(犘<0.05); andincreasedtheantioxidantenzymesactivitiesandleafrelativewatercontentsofthe
bentgrassunderdroughtstress(犘<0.05).Theseresultsindicatedthatcytokininimprovedthedrought
toleranceofcreepingbentgrass.Inthelatestagesofdroughtstress,theindicatorsofcreepingbentgrass
withhighconcentration(100μM)cytokinintreatmentshowedsignificantadvantages(犘<0.05).These
resultsindicatedthatthehighconcentrationofcytokininhadabetterpromotiontocreepingbentgrassun
derdroughtstress.
犓犲狔狑狅狉犱狊:Cytokinin;Drought;Creepingbentgrass;Antioxidativephysiology
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TotalN
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TotalP
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TotalK
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Fig.1 Soilmoistureconditionsofdroughtregime
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Table2 ImpactofTZRontheleafrelativewatercontentsofcreepingbentgrassattwomoistureregimes
x¾@
Moisturetreatments
TZR¾@
TZRtreatments/μM
Mhpºrelativewatercontents/μmol·g-1
9%3030Sep.
10%1010Oct.
10%2020Oct.
10%3030Oct.
11%99Nov.
ñxÜ
Welwatered
0 89.91a 89.89ab 87.85bc 87.78b 86.80bc
10 90.64a 90.28ab 88.06b 88.85b 88.36b
100 91.59a 91.53a 90.73a 91.01a 90.12a
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Drought
0 91.13a 88.73c 83.63d 76.48f 66.13f
10 89.95a 89.15b 86.73c 79.20e 71.52e
100 91.07a 90.29ab 88.09b 81.24d 74.68d
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Table3 ImpactofTZRonH2O2contentsofcreepingbentgrasssubjectedattwomoistureregimes
x¾@
Moisturetreatments
TZR¾@
TZRtreatments/μM
H2O2/μmol·g-1
9%3030Sep.
10%1010Oct.
10%2020Oct.
10%3030Oct.
11%99Nov.
ñxÜ
Welwatered
0 22.90a 24.96b 24.73c 21.90d 21.43d
10 21.15a 22.75bc 21.43d 20.38d 19.35d
100 21.10a 21.70c 17.20e 17.75e 14.50e
%t
Drought
0 22.63a 27.63a 36.12a 38.63a 62.63a
10 22.23a 27.38a 32.10b 34.35b 46.63b
100 21.98a 24.88b 25.80c 30.05c 41.03c
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Table4 ImpactofTZRontheO-2 productionrateofcreepingbentgrasssubjectedattwomoistureregimes
x¾@
Moisturetreatments
TZR¾@
TZRtreatments/μM
O2-/μmol·g-1·min-1
9%3030Sep.
10%1010Oct.
10%2020Oct.
10%3030Oct.
11%99Nov.
ñxÜ
Welwatered
0 17.08a 17.57abc 19.86c 19.60d 16.74d
10 16.94a 17.07bc 17.22d 17.09e 14.96e
100 17.02a 16.33cd 15.82e 15.50f 12.74f
%t
Drought
0 16.86a 18.87a 24.31a 26.43a 27.99a
10 17.52a 17.70ab 20.90b 24.05b 26.60b
100 16.98a 15.76d 18.21d 22.51c 22.98c
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Table5 ImpactofTZRontheMDAcontentsofcreepingbentgrasssubjectedattwomoistureregimes
x¾@
Moisturetreatments
TZR¾@
TZRtreatments/μM
MDA/μmol·g-1
9%3030Sep.
10%1010Oct.
10%2020Oct.
10%3030Oct.
11%99Nov.
ñxÜ
Welwatered
0 34.37a 37.54ab 42.54d 41.55d 43.34d
10 35.29a 36.06b 42.06d 40.13d 40.92d
100 35.20a 35.01b 36.91e 34.41e 36.12e
%t
Drought
0 35.46a 36.92ab 70.46a 114.06a 116.08a
10 36.57a 39.31a 51.16b 91.17b 106.79b
100 36.43a 34.94b 46.17c 49.91c 71.49c
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Fig.3 CATactivityofcreeping
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underdifferenttreatments
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