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Effects of PGPR Inoculum without Exogenous Nitrogen on the Growth and Root System Characteristics of Vicia sativa L.

无外源氮素条件下接种促生菌对箭筈豌豆生长及根系特性影响



全 文 :!23" !3#
 Vol.23  No.3
! " # $
ACTA AGRESTIA SINICA
   2015$  5%
  May.  2015
犱狅犻:10.11733/j.issn.10070435.2015.03.010
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3.8Ë]4)*÷v)°’“ÌÍ89,ÆÇ ò`730070)
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:G3+Y16+J31)>azc,|<6`a;6Ñ、4†ñ&"、4ñ&"、ˆ·、ˆ+Ž、ˆ2、ˆuv
0¿Qˆù!.J*S<àùú45.75%,177.16%,211.69%,73.7%,238.9%,199.2%,12.4%j44.2%。õ
öÇ¥ˆbc)
(G3)+,q¶DË5¬èI‡ñ>a,-DË5<ˆù!.¦Ñœ‹,nP$dˆbc+˜+
Œc+øÅc6I¡O`)x_¢#ÌO6`a;ñ&ckIzcc6«。
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:S551.9    >?@AB:A     >CD=:10070435(2015)03049606
犈犳犳犲犮狋狊狅犳犘犌犘犚犐狀狅犮狌犾狌犿狑犻狋犺狅狌狋犈狓狅犵犲狀狅狌狊犖犻狋狉狅犵犲狀狅狀狋犺犲犌狉狅狑狋犺犪狀犱
犚狅狅狋犛狔狊狋犲犿犆犺犪狉犪犮狋犲狉犻狊狋犻犮狊狅犳犞犻犮犻犪狊犪狋犻狏犪犔.
MAWenbin1,2,YAOTuo1,2,3,RONGLiangyan1,2,WANGGuoji1,2,
SUNGuangzheng1,2,LIUHuan1,2
(1.PrataculturalColege,GansuAgriculturalUniversity,Lanzhou,GansuProvince730070,China;
2.KeyLaboratoryofGrasslandEcosystem,MinistryofEducation,Lanzhou,GansuProvince730070,China;
3.SinoU.S.CentersforSustainableDevelopmentofGrasslandandAnimalHusbandry,Lanzhou,GansuProvince730070,China)
犃犫狊狋狉犪犮狋:ThreePGPR(StrainNo.:G3,Y16,J31)strainswereisolatedandscreenedfromtherhizo
sphereofvetch(犞犻犮犻犪狊犪狋犻狏犪).Bothsingleinoculumandcompoundinoculumweredesignedwiththese
strains.Effectofinoculumonthegrowthandrootsystemcharacteristicsofvetchwasdeterminedbyanin
doorexperiment.Theresultsindicatedthattheplantheight,biomass,diameter,rootlengthandrootac
tivityofvetchincreasedafterusinginoculants.TheeffectivenessoftreatmentD(phosphatedissolving+
nitrogenfixing+rhizobiainoculants:G3+Y16+J31)wasbetterthansingleinoculant.Theplantheight,
abovegroundbiomass,undergroundbiomass,rootlength,surfacearea,rootvolume,rootdiameterand
rootactivityincreasedby45.75%,177.16%,211.69%,73.7%,238.9%,199.2%,12.4%and44.2%,
respectively.Meanwhile,A(G3)wasthebestsingleinoculum.Thecompoundinoculum (G3+Y16+J3
1)wasrecommendedasthebestresourcestosubstituterhizosphereinoculumfor犞犻犮犻犪狊犪狋犻狏犪 .
犓犲狔狑狅狉犱狊:犞犻犮犻犪狊犪狋犻狏犪L.;Plantgrowthpromotingrhizobacteria(PGPR);Inoculum;Biomass;Root
systemcharacteristics
EFGH
:20140506;IJGH:20141216
KLMN
:¸
¹_ö2Ê
(31360584);,Ú®÷Í÷„…2ù(CARS35);ÆÇ(®÷-„ி¥(GNCX201245)ÀÁ
OPQR

›Ó®
(1989),á,ÆÇÒb§,úËÌÍñ,ÌÍփ_]4„ñ&À?¶ñò,Email:843606723@qq.com;:ex×
Authorforcorrespondence,Email:yaotuo@gsau.edu.cn
  6`a;(犞犻犮犻犪狊犪狋犻狏犪L.)˜f、]、c、kg
¤x&g¥

ðâSÑIQ«ïð

S/I^ÒHñ
Q©­ñ

©ªZ"ÞÑÒÑw4@

è$:

ü
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fÑÒ®*
!3# ›Ó®:ÿC?Œ¥NOO`‡ñc<6`a;ñ·QˆùHñÕÖ
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(犃狏犲狀犪狊犪狋犻狏犪L.)§f,¤ÞÑ¡yc,â
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[1],
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˜
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ˆù˜YBÉkÀ?I0O£¤×

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&5(

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ix

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[79]
I
âJc

è$:
,¸
åC.×<ÞPGPRIJ‚º
p

ñ5ñ\Hñ

Jrñ&.HñPQ£¤PGPR
OxI„ñ&O`)
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D*T֎IÌÍS_®¬

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ž&ˆùMòIÕÖÌÍĪ¸|

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6`a;_ÌÍ<Ä

£¤á#J‚ºpqIoÂ
ˆL‡ñcÌO„ñ&O`)

€x+2"«n
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_6`a;Ñ>£¤Œ¥Q«¦R5’LHj
„…º»

1 STUVW
1.1 gÖST
J‚_6`a;j[hˆL‡ñc26,ˆbc1
6

ÝÆÇ®÷à.]÷./„ñ&“îÞ¦R

c6
Hñª+1。6`a;`r,»`_¡6`a;(犞犻犮犻犪
狊犪狋犻狏犪),ÝÆÇ®÷à.]÷./`rÞ¦R。
q1 hicjbÑÝÐ
Table1 CharacteristicsoftestedPGPRstrains
c6U
Straincode
omž&
Hostplant
‡ñHñ
Function
+Œ£!ñ
Nitrogenaseactivity
/C2H4nmol·m-1·h-1
øÅ"
Psolubilization
capacity/mg·L-1
Jˆñ·¥
IAAsecreting
Ability/μg·mL-1
J31
6`a;
犞犻犮犻犪狊犪狋犻狏犪
øÅ
Psolubilization
- 523.5 12.01
Y16
[h
犣犲犪犿犪狔狊
øÅ


Psolubilization,
Nfixation
178.94 178.2 17.8
G3
6`a;
犞犻犮犻犪狊犪狋犻狏犪

Nfixation
373.52 - -
1.2 ÉéK
LB[9]:¤Þ!\Qéîc`;YMA[9]:¤Þ"
«ˆbc
;HoaglandÿŒ"«2[10]:¦R6`a;
„…üŒ¥CQ«

Hoaglandÿ Œ " « 2 { Ö (1000 mL):1
mol·L-1 MgSO4·7H2O4mL,1mol·L-1KH2PO4
2mL,1mol·L-1CaCl2·2H2O10mL,1mol·L-1
KCl10mL,Fe·EDTA2mL,„"Ê¥Q«n2mL,
Agar2g。
„"Ê¥Q«n{Ö
(500mL):HBO30.310g,
MnSO41.115g,ZnSO4·7H2O0.430g,NaMoO4·
2H2O0.0125g,CuSO4·5H2O0.00125g,CoCl2
0.00125g,KI0.0375g。
1.3 cjklm®
c6hIp^.­í°¤Jqr³
[11],

`cfn2"«28"«24hIcsn,P2%
(V/V)I " O ` Ø ’ t " « 2 8,28℃,180
r·min-1Âu"«48h;’tn8000r·min-1‚9
10min,†ç;0.22μmÞv,“Þn100μLwú
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Table2 Treatmentsforexperiment
UNumber Ë5Treatments Á•Comments
A G3 ˆbcO`)(»9ˆbc))Rhizobiainoculants
B Y16 ˜+ŒcO`)(»9˜+Œc))Singlenitrogenfixinginoculants
C J31+Y16 øÅc+˜+Œc¡O`)(»9¡O`)Ⅰ)Phosphatedissolving+nitrogenfixinginoculants
D G3+Y16+J31
ˆbc+˜+Œc+øÅc¡O`)(»9¡O`)Ⅱ)
Phosphatedissolving+nitrogenfixing+rhizobiainoculants
E ÿcÉSterilewater <àControl
1.4.2 EJ p“Öq}2、à4¥ŠI¡6
`a;`r

W`r>¤75%I~”gcË5
15s,ö¢¤ÿcÉìO4~5~,!í0.1%
HgCl2 gcË51min¢,¤ÿcÉìO¹õ,W
`ròÞgc¢IÉ€"«2ux8

òÞ"
«R
(25℃,16h=à,20000lx;20℃,8hX)
8"«

Ô`r‚·1~2cm,W6`a;„…%
C©â50mLÿc Hoaglandx+2ÿŒ"«2
I·ƒ„íÔ
(4cm×45cm)8,‘AíÔ16
„…

‘AË55~~¡,‹1«<à。òÞ"
«R8"«
,2d¢††,ìü%Z¢„ÐI„
…

1.4.3ÄE W1.4.18)csn1mLfÿcN
OO¬1.4.2n"«I„…ˆL,O`¢WíÔ
‰°òÞ"«R8"«

1.4.4 ÅAÆÇÈ|} íË5I„…"«
40d¢Óô,¤ÿcÉOõˆ+"«2,"6
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ò ì 4 † U J

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UJÄ~

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5WÖãJK

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2 mn,c
2.1 a„nÁdeføÑ"ÔÕ
2.1.1 ÄEÉÊËÌÍÎÏÐÑÒ :;ˆO
`)<6`a;6ÑÕÖ

Ý+3KÀ,¶EË5
(CK,Œ)¬t,)O`)<6`a;I6ÑÕÖ
)R

O`c)Iž6Ñeœ‹ÑÞdO`ž6Ñ
e
(犘<0.05)。¶CK¬t,Ë5A,B,CjD£ž
66ÑJ*ùú‰39.55%,15.32%,47.05%,
45.75%,|8Ë5C<ž66ÑI‡5>az_
›œ

|~˜Ë5DjË5A,-=×ãR‹œ‹,
Ë5B¶|ýO`Ë5ghîfœ‹ãR(犘<0.
05),<6ч5x¤z4。
2.1.2 ÄEÉÊËÌÍÎkrsÑÒ Ï+3vÚ
q

‹ŒO`)Ë5œ‹ÕÖ6`a;Iñ&"
(犘
<0.05)。¶CK¬t,Ë5A,B,CjD£6`a;
4†ñ&"J*ùú44.42%,42.83%,79.51%,
177.16%,|8Ë5D<ž64†ñ&"ÕÖz_œ
‹
(犘<0.05),|~_CË5;²2:Ú,¡O`)
<4†ñ&"ÕÖoÞÇ¥O`)

ç2`Ç¥O`
)Ë5ãR‹œ‹

æO`)Ë5<ž64ñ&
"ÕÖ²2ÑÞ4†ñ&"

)O`Ë5SCK¬t
ãRœ‹
(犘<0.05),J*£ž64ñ&"ùú‰
160.37%,30.88%,128.53%,211.69%,|8Ë5D
<ž64ñ&"IÕÖzà

Ë5AjC~g,Ë
5Bz4。³†n;,O`)Ë5<6`a;4†j
4UJvâÂ2I‡5x¤

894
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Table3 EffectsofPGPRinoculumontheheightandbiomassofvetch
Ë5
Treatments

Plantheight/cm
4†ñ&"
Abovegroundbiomass/g
4ñ&"
Undergroundbiomass/g
A 42.80±0.76a 0.2276±0.0045c 0.3765±0.0108b
B 35.37±2.14b 0.2251±0.0262c 0.1894±0.0089d
C 45.10±0.38a 0.2829±0.0071b 0.3319±0.0144c
D 44.70±0.38a 0.4368±0.0050a 0.4507±0.0075a
E 30.67±1.20c 0.1576±0.0100d 0.1446±0.0032e
  •:+8GH_uvð±{%©;Œæ‹Œ4…wx+ÅãRœ‹(犘<0.05),Œ
Note:Means±SD;Differentsmallettersindicatesignificantdifferenceatthe0.05level,thesameasbelow
2.2 a„nÁdeføë0ope@ÔÕ
:;ˆO`)<6`a;mˆ·eÕÖ+›

+4),O`)Ë5<ˆ·ÕÖ)R,|8A,CjD
Ë5SCKãRœ‹(犘<0.05),J*SCKùú‰
33.4%,61.1%j73.7%,BË5ˆ·‹QCK。
Ý+4vÀ,:;O`Ë5,6`a;ˆù+Ž
Q2vœ‹ùú

)Ë5ž6ˆù+ŽI
ùú"L~_D>A>C>B>E,J*˜CKI3.39
Y
,3.35Y,1.95YQ1.06Y,|8Ë5A,C,D¶
CKãRœ‹(犘<0.05),BË5¶ CKãR‹œ
‹

-SCKùú‰5.8%;)Ë5<ˆ2jˆ+
ŽÕ֔•2?¥Š

O`Ë5SCK¬t<ˆ
2Iù‚_35.2%~199.2%。
)Ë5<ˆùuv0¿Õ֋ŒÞ|ýMòz
{

<ˆùuv0¿Iùú"_B>C>A>D>E,
J*SCKùú45.9%,40.5%,15.3%j12.4%,
Ë5BjC¶A,D,EãRœ‹(犘<0.05),AQD
Ë5¶CKãR‹œ‹。
q4 a„nÁdeføë0opÔÕ
Table4 EffectsofPGPRinoculumontherootsystemcharacteristicsofvetch
Ë5
Treatments
mˆ·
Rootlength/cm
ˆ+Ž
Rootsurfacearea/cm2
ˆ2
Rootvolume/cm3
ˆuv0¿
Rootdiameter/mm
A 9.83±0.32b 27.1385±0.5047a 0.276±0.022b 0.5441±0.0337b
B 4.77±0.49d 8.5634±0.7686c 0.165±0.010c 0.6888±0.0092a
C 11.87±0.95a 15.7441±0.9884b 0.257±0.023b 0.6634±0.0316a
D 12.80±0.78a 27.4316±0.2559a 0.365±0.005a 0.5307±0.0089b
E 7.37±0.32c 8.0944±1.4230c 0.122±0.011c 0.4720±0.0141b
2.3 a„nÁdeføë0€q%áë"ÔÕ
x&ˆù˜Ý‹Œ`+Iˆ«n

Wˆù0¿
f0~1.0mm åIˆˆ¨_+ˆ[12]。Ý+5v
ª

O`)<0~1.0mmå+ˆˆ·Õ֜‹。D
Ë50~0.5mmåIˆ·œ‹ÑÞ|ýË5,Bj
CË5°‹QCK。JK‹ŒŽ#ˆ·f)y*I
JtuvÀ90%P†I6`a;ˆ˜f0~1.0
mmI0¿LMå,!›+ˆIø‡½ˆˆ·Ià
4

)Ë5f0~1.0mmåIˆ·tuP/âœ
‹ãR

‰1)。nP,O`)Ë5<ˆ·Iùúx
¤mV˜+ˆ·eIùú

拘|tuIùà

q5 a„nÁdeføë0€q%áGë"ÔÕ
Table5 Effectsofinoculumontherootlengthsofdifferentrootdiametersofvetch cm·6-1
Ë5Treatments
ˆ0¿Rootdiameter/mm
0~0.5 0.5~1.0 ˆ1.0
A 111.4958±8.0004b 43.1878±3.9658a 5.6743±1.8249a
B 24.6999±2.2622e 19.7538±3.0940c 4.0281±0.6947ab
C 44.6025±2.6268d 25.9447±1.9794bc 5.7421±0.7669a
D 142.0951±7.4551a 30.7562±1.1313b 4.4874±0.4607ab
E 67.4278±2.3855c 8.2123±0.5251d 1.7330±0.2226b
2.4 a„nÁdeføë0rÓÔÕ
߉2nÅ,‹ŒO`)Ë5,6`a;ˆù!
.—\)R

<¡O`)D¶CK¬tãRœ‹(犘
<0.05),˜CKI1.44Y,BjCË5¶CKÿœ‹ã
R

-SCKˆù!.J*¦Ñ‰8.42%,16.84%,A
Ë5ˆù!.ƒ

-¶CKãR‹œ‹。
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Fig.1 Effectsofinoculumontherootdiameterofvetch
<2 a„nÁdeføë0rÓÔÕ
Fig.2 EffectsofPGPRinoculumontherootactivityofvetch
3 œ
3.1 sѺa„nÁˆºÑ"bÆOt
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åC.×Û)`O`)<ž&Í"j»ÀÕÖ5W
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fYB~e¹NO

£¤PGPRc)‰`¸;`r,v¦áq…,‡5
„…ñ·

ùú¸;ž&"QŠaG

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<Œ;IÌÍ+›

O`â`‡ñc¢Œ;6ÑJ
*t<à¦Ñ14.39%j10.40%;K2¹&~J
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+Žjñ&"
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žŸ>?
[1] ­¢.„ÖƒÍYB“¤ñkx&Z"¶£¤[M].Èq:È
q-„s‘qÁ›r
,2010:60
[2] MarschnerH,KirkbyEA,CakmakT.Effectofmineralnu
tritionalstatusonshootrootpartitioningofphotoassilatesand
cyclingofmineralnutrients[J].JournalofExperimentalBota
ny,1996,47(12):12551263
[3] WangY,MiGH,ChenFJ,犲狋犪犾.Responseofrootmorphol
ogytonitratesupplyanditscontributiontonitrogenuptakein
maize[J].JournalofPlantNutrition,2004,27(12):21892202
[4] iP$,ý¡½,ü¹,.Œ¥j¡<Ñ4Eˆùñ·’;I
ÕÖ
[J].x&.¸,2003,29(6):913918
[5] –Ï’.ž&Q«ñòñ5.jãä.[M].„À:8¸-„
qÁÂ
,1993.2163
[6] AdesemoyeA O,TorbertH A,KloepperJ W.Enhanced
plantnutrientuseefficiencywithPGPRandAMFinaninte
gratednutrientmanagementsystem [J].CanadianJournalof
Microbiology,2008,54(10):876886
[7] OliveiraCA,AlvesVMC,MarrielIE.Phosphatesolubiliz
ingmicroorganismsisolatedfromrhizosphereofmaizecultivat
edinanoxisoloftheBrazilianCerradoBiome[J].SoilBiology
andBiochemistry,2009,41(9):17821787
[8] ChenZX,MaSW,LiuL.Studiesonphosphorussolubilizing
activityofastrainofphosphorbacteriaisolatedfromchestnut
typesoilinChina[J].BioresourceTechnology,2008,99(14):
67026707
[9] HafeezFY,MalikKA.Manualonbiofertilizertechnology
[M].NIBGE,Pakistan,2000:5051
[10]MalikKA,BilalR.SurvivalandColonizationofInoculated
BacteriainKalarGrassRhizosphereandQuantificationofN2
Fixation[C]//SkinnerF.A,BodderandR.M,FendrikI.Ni
trogenFixationwithNonlegumes.KluwerAcademicPublish
ers,TheNetherlands,1989:301310
[11]ˆà›,–Ò“,”,.p^c6 Kct99I@ˆQ|Xc
!ñÌÍ
[J].ñ&„…,2011(4):153157
[12]WhitingSN,NeumannPM,BakerJM.Applyingasolute
transfermodeltophytoextraction:Zincacquisitionby犜犺犪狊狆犻
犮犪犲狉狌犾犲狊犮犲狀狊[J].PlantandSoil,2003(1),249:4556
[13]Agg,A•–,­l²,.4APGPRc6‰`<¹NO
¸;ñ·QÍ"IÕÖ
[J].¹@ÌÍ,2012,29(2):203
207
[14]̋´,—(,§úº,.â6PGPR<Œ;…#ñ·QY‹
+c™šIÕÖ
[J].®÷(-..¸,2012,31(8):1537
1543
[15]è²,l4U,–f—,.ÑÒ4@eEˆL˜+ŒcÌ
Í.+Œc2004,13(10):101105
[16]ÃÒ«,.¸,~&Å,.4lÙ¦“nQˆbc<42á
s℅ñ·IÕÖ
[J].]÷-.,2011,28(4):572576
[17]JosLB,AlfredoCR,LuisHE.Theroleofnutrientavaila
bilityinregulatingrootarchitecture[J].CurrentOpinionin
PlantBiology,2003,6(3):280287
[18]§\ï,µÒù,˜«’,.±Œ<à;ˆùMòjŒ¥0Ó
çIÕÖ
[J].8¸ñò®÷.¸,2009,17(6):10691073
[19]Nn,§Ó›,P(ž,.¡¥"«<à;Q«ñ·jˆù
MòIÕÖ
[J].ž&Q«¶kâ.¸,2010,16(4):1032
1036
[20]™ï,š›’,§œÓ,.Œ、Å、¿Q«4#!.QˆùŸÉæIÕÖ
[J].8¸ñò®÷.¸,2007,15
(6):6972
[21]§1,¦.AM kc[J].!Ÿ®÷-.,2012,40(1):1314
[22]¬Ó«,§X£,L¯/,. ¡<Éô¢„…+ˆ=VIÕ
Ö
[J].ž&ñò.¸,2006,30(1):104111
[23]ÞÉ/,§X£,êÕí,.Éô¢jš2n+ˆœíI3¼
[J].ž&ñò.¸,2007,31(1):102109
[24]ÔÏn.ÆÇÒ@\]ˆbc‡ñô.ÕÖürJKQÑ
>‡ñc6ºpÌÍ
[D].ò`:ÆÇ®÷à.,2005:5

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