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作 者 ：陈彩霞, 崔思然, 江艳华, 李艾莲

期 刊 ：植物科学学报 2012年 30卷 3期 页码：315-321

关键词：金荞麦；愈伤组织诱导；器官分化；植株再生；

Keywords:Fagopyrum dibotrys, Callus induction, Organogenesis, Plant regeneration,

摘 要 ：目前转基因技术已成为植物定向遗传改良的重要手段,而建立稳定高频的离体再生系统是实现遗传转化的基础和前提。本试验以25~30 d苗龄的金荞麦(Fagopyrum dibotrys)无菌苗叶片、茎节间、叶柄为外植体进行愈伤组织诱导与植株再生研究。结果表明: 叶片在MS+2,4-D 4.0 mg/L+6-BA 1.0 mg/L培养基上愈伤组织诱导率达到89%。茎节间在 MS+2,4-D 2.0 mg/L+6-BA 2.0 mg/L培养基上愈伤组织诱导率为87%。叶柄在MS+2,4-D 4.0 mg/L+6-BA 2.0 mg/L+IBA 0.2 mg/L培养基上的最高诱导率仅为54%。愈伤组织分化不定芽的适宜培养基为MS+6- BA 2.0 mg/L+TDZ 0.2 mg/L+NAA 0.2 mg/L;金荞麦不定芽在1/2 MS+NAA 0.5 mg/L的培养基上生根效果最好。组培再生植株经炼苗后移栽到田间成活率达80%以上,且生长表现正常。高频完整再生体系的建立,为金荞麦进一步遗传操作和扩大药材资源奠定了基础。

Abstract:Transgenic technology has now become one of the most important measures in plant directional genetic improvement,while the establishment of a high-efficiency regenerating system must be the basis and prerequisite of genetic transformation.In the present research,explants (leaf,petiole and stem segments) excised from 25-30 day-old in vitro seedlings of Fagopyrum dibotrys were used as materials for inducing callus and plant regeneration.Results showed that MS+2,4-D 4.0 mg/L+6-BA 1.0 mg/L was beneficial for leaves to form callus (89%).Internodal cuttings formed callus (87%) on MS medium supplemented with 2,4-D 2.0 mg/L+6-BA 1.0-2.0 mg/L,and the highest callus induction from the petiole was only 54% on MS+2,4-D 4.0 mg/L+6-BA 2.0 mg/L+IBA 0.2 mg/L media.The medium of MS+6-BA 2.0 mg/L+TDZ 0.2 mg/L+NAA 0.2 mg/L was prior to callus differentiation and shoot organogenesis.The optimum medium for producing roots (96.67%) was half-strength MS medium supplemented with NAA (0.5 mg/L).Hardened plantlets via acclimatization were successfully transplanted to a field (80%),with the regenerated plants normal morphologically and in growth characters.The high-efficient stable organogenesis regeneration system of Fagopyrum dibotrys will make it possible for genetic transformation and enlarging medical resources for this species.

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