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作 者 ：孟昱;张钢;李雪平;高志民

期 刊 ：武汉植物学研究 2010年 28卷 3期 页码：341-346

关键词：毛竹叶片；NaCl胁迫；电阻抗图谱；膜透性；脯氨酸；

Keywords:Moso bamboo leaves, NaCl stress, Electrical impedance spectroscopy, Membrane permeability, Proline,

摘 要 ：

以NaCl胁迫下毛竹(Phyllostachys edulis)实生苗叶片为材料,测定其电阻抗图谱参数和膜透性的变化,通过膜透性与电阻抗图谱参数间的相关性,来证明电阻抗图谱法研究竹子受胁迫程度的有效性.结果表明:随着盐浓度的升高,胞外电阻、胞内电阻和弛豫时间呈现先减小、后增加、再减小的特征,而弛豫时间分布系数表现恰好相反;叶片细胞膜相对透性逐渐增大,脯氨酸的含量先逐渐增大,然后减小.相关分析表明:胞内电阻、胞外电阻、弛豫时间与脯氨酸含量呈极显著负相关(p<0.01);胞外电阻、弛豫时间与膜相对透性呈显著负相关(p<0.05).电阻抗图谱参数能够有效地表示毛竹受NaCl胁迫的程度,电阻抗图谱法将是竹子逆境胁迫研究的一种有效方法.

Abstract:The relationship between electrical impedance parameter and relative electrolyte leakage of Moso bamboo (Phyllostachys edulis) seedling leaves under NaCl stress was measured. Results showed that with the increasing concentration of NaCl, extracellular resistance, intracellular resistance, and relaxation time initially decreased, then increased, and then finally decreased again. Conversely, the distribution coefficient of relaxation time showed the opposite pattern. Membrane permeability increased gradually, while proline firstly increased then decreased. There were significant negative correlations among extracellular resistance, intracellular resistance, relaxation time, and proline content. A significant negative correlation was found among extracellular resistance, relaxation time, and membrane permeability. These results indicate that the extent of Moso bamboo leaves stressed by NaCl was reflected effectively through electrical impedance parameter spectroscopy, which is an effective method to study bamboo adversity stress.

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