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作 者 ：秦永燕;王祎玲;张钦弟;毕润成;闫桂琴

期 刊 ：武汉植物学研究 2010年 28卷 4期 页码：466-472

关键词：翅果油树；SSR；遗传多样性；遗传结构；

Keywords:Elaeagnus mollis, Simple Sequence Repeats, Genetic diversity, Genetic structure,

摘 要 ：利用微卫星(SSR)标记对来自山西和陕西两省的7个翅果油树(Elaeagnus mollis Diels)种群进行遗传多样性和遗传结构研究。10对SSR标记共检测到126个位点,其中多态位点114个。在物种水平上,平均多态位点百分率为90.79%,有效等位基因数(N_e)、N_ei基因多样性指数(H)和Shannon多样性指数(Ⅰ)分别为1.6072、0.3166、0.4603;在种群水平上,多态位点百分率为61.99%,有效等位基因数(N_e)、Nei’s基因多样性指数(H)、Shannon多样性指数(Ⅰ)分别为1.5445、0.2683、0.3815。遗传分化系数G_ST为0.2074,表明了翅果油树种群的遗传变异主要存在于种群内。基因流N_m为1.9111>1,说明种群间基因交流可以阻止由于遗传漂变导致的遗传分化。聚类结果表明,翅果油树种群间的遗传距离与地理距离有一定的相关性,经Mantel检验,种群的地理距离与遗传距离之间呈正相关,但未达到显著水平(p>0.05)。结果表明,遗传多样性水平与物种本身特性和不同干扰生境有关,濒危植物并不一定表现为遗传变异水平的降低。

Abstract:Simple sequence repeat(SSR) markers were used to estimate the genetic structure and diversity of seven populations of Elaeagnus mollis,an endangered plant from the Shanxi and Shaanxi provinces,China.Ten pair primers generated a total of 126 reproducible and discernible loci,among which 114 were polymorphic.At a species level,the proportion of polymorphic loci was 90.79%.The effective number of alleles(N_e) was 1.6072,Nei’s gene diversity index(H) was 0.3166,and the Shannon index(Ⅰ) was 0.4603.At a population level,the proportion of polymorphic loci was 61.99%,N_e=1.5445,H=0.2683,and Ⅰ=0.3815.The coefficient of gene differentiation(G_st=0.2074) showed greater genetic variations occurred within the population than among populations.Gene flow(N_m) was 1.9111>1,which showed that gene interflow occurred among populations,and that genetic differentiation was prevented due to genetic drift.Cluster analysis showed a certain correlation between genetic distance and geographic distance.Mantel test showed a positive correlation between geographic distance and genetic distance,but did not reach a significant level(p>0.05).The results showed that genetic diversity was related to species traits and habitat disturbance.The endangered plants did not necessarily display a lower level of genetic variation.

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