Abstract:Optimum media for Ginkgo biloba L.leaf callus induction and successive transfer culture were investigated.Callus that arose from Ginkgo leaf explants was used as the research materials,and two varieties of active integrants,superoxide dismutase(SOD) and total flavones,were compared between leaves and its callus.The results demonstrated that SOD activity in callus was markedly higher than that in leaves,as were SOD isoenzymes.However,the content of flavones in callus was lower than that in leaves.The fact that SODs and flavones can scavenge the production of superoxide anion indicated that different predominant mechanisms were used to resist free radicals in Ginkgo callus and leaves.